Studies on dormancy, ecology, phytotoxic effects, competition and chemical control of Rhynchosia capitata (Roth) DC in mungbean (Vigna radiata (L.) Wilczek) were investigated in the laboratory experiments at Department of Agronomy, University of Agriculture, Faisalabad and under farmer’s field conditions. In laboratory experiments different treatments for breaking dormancy of R. capitata seeds were evaluated. Seeds were soaked in thiourea, KNO 3 , HCl, HNO 3 and H 2 SO 4 as well as scarified mechanically (sand paper). Results indicated that seeds of R. capitata show signs of physical dormancy that is mainly because of impermeability of their coat. Mechanical scarification, acid scarification (soaking of seeds in H 2 SO 4 for 60 and 80 and in HCl for 12 and 15 h) were very effective in breaking dormancy and promoting germination. Seed soaking in HNO 3 for 1 to 5 days showed little effect, while, various concentrations of thiourea and KNO 3 were ineffective in breaking seed dormancy of R. capitata. Germination response of R. capitata to environmental factors such as temperature, salt stress, drought stress, pH, light and seeding depth were also studied. Germination increased as the temperature increased from 25 o C and considerably reduced at 45 o C. Germination of R. capitata seeds was not influenced by presence or absence of light. Increase in salt stress, moisture stress and seed burial depth significantly decreased the seed germination of R. capitata. Seeds of R. capitata had ability to germinate over a wide range of pH (5-10). In seed burial trial, maximum seedling emergence of 93 % was recorded at 2 cm depth, and seedlings failed to emerge from a depth of 12 cm. In third laboratory experiment, allelopathic influence of R. capitata on germination and seedling growth of mungbean along with detection of the phytotoxic materials liable for this action were studied. Aqueous extracts of root, shoot, leaf, fruit and whole plant (5%) adversely affected germination and seedling growth of mungbean, but higher inhibition was seen with R. capitata leaf water extract. A linear decrease in the germination characteristics of mungbean was observed with the decrease in the concentration of leaf extract from 5% to 1%. The soil incorporated residues (1-4% w/w) of R. capitata stimulated the development of root and hypocotyl at low concentrations and inhibited their development at elevated concentrations. Rhynchosia capitata soil incorporated residues (4% w/w) significantly reduced the seedling vigour index of mungbean in addition to its significant effect on total germination. A noteworthy amount of water soluble phenolic acids were found in extracts of different plant parts of R. capitata. Total phenolic acids were greater in leaf extract compared to that of stem, fruit or root extracts. Two phenolic acids, vanillic acid and 4-(hydroxymethyl) benzoic acid were found in R. capitata leaf extract. Effect of different weed crop competition periods i.e. full season competition, weed crop competition for 3, 4, 5, 6, 7 weeks and zero competition were studied under field conditions on mungbean. The results showed that full season weed competition produced highest weed fresh and dry weight, maximum NPK contents (%) and NPK uptake, highest Fe, Mn, Na, Zn, Ca, Cu and Mg contents and micronutrients uptake by R. capitata in both the years of study. The maximum plant height, number of pods per plant, grain number per pod and 1000-grain weight of mungbean was recorded in weed free plots. Increase in competition period decreased above parameters of mungbean significantly. Increase in R. capitata competition period decreased the mungbean grain yield significantly. In 2011, the weed-free plots gave the highest grain yield of 1688.6 kg ha -1 followed by competition 3 weeks after planting with 1582.0 kg ha -1 of seed xxiyield. The full season R. capitata competition decreased the grain yield to 869.3 kg ha -1 . Similar trend was also observed during 2012. In second field experiment, the efficacy of herbicides namely pendimethalin + prometryn @ 875 g, 700 g and 525 g a.i ha -1 , S- metolachlor @ 1440 g a.i ha -1 and pendimethalin @ 825 g a.i ha -1 in controlling R. capitata was evaluated. All doses of the herbicides suppressed the dry biomass of R. capitata from 60 to 78% in 2010 and 2011. Pendimethalin+prometryn @ 875g a.i. ha -1 , recorded (74% in 2010 and 78% in 2011) maximum reduction in total weed dry weight. Among herbicide treatments, maximum grain yield was recorded with pendimethalin+prometryn @ 875 g a.i. ha -1 in both the years. Pendimethalin + prometryn @ 875 g a.i. ha -1 proved best treatments for effective control of R. capitata in mungbean and to get maximum economic benefits.
تنقید: تنقید عربی زبان کا لفظ ہے۔جس کے معنی جانچنا، پرکھنا ، کھرے اور کھوٹے کو الگ کرنا ہیں۔ تنقیدکی ضرورت و اہمیت: تنقید کا سلسلہ تخلیق آدم سے شروع ہوا۔ جب اللہ تعالی نے حضرت آدم ؑ کو پید اکیا تو فرشتوں نے اللہ سے گزارش کہ کہ یہ انسان دنیا میں جاکر فساد اور لڑائی جھگڑے کرے گا۔ اس کی تخلیق کی ضرورت کیوں پیش آئی؟عبادت کے لیے تو ہم کافی ہیں۔ اس کی تخلیق پر نظر ثانی کی جائے۔اللہ نے فرشتوں کوغرض تخلیق آدم? سے آگاہ کیا۔سب سے پہلے آدمؑ پر تنقید کی گئی یعنی تخلیق پر تنقید ہوئی۔ نقاد بھی اسی دن پیدا ہوگیا تھا جس دن پہلی تخلیق ہوئی تھی۔اس حوالے سے پہلا شخص سقراط تھا۔ اس سے فلسفے کی بنیاد پڑی۔اس نے اپنے ہاتھ سے کچھ نہیں لکھا لیکن اس کی باتوں کو اس کے شاگرد افلاطون نے مکالمے کی شکل میں لکھا اور اس کتاب کا نام" مکالمات افلاطون"ہے۔اس کتاب میں زندگی کے تمام موضوعات پر بات کی گئی ہے۔ اس میں ایک مثالی ریاست کے موضوع پر بھی بات کی گئی ہے۔یوں ہم کہہ سکتے ہیں کہ تنقید کی ابتداء یونان سے ہوئی۔ بوطیقا ارسطو کی کتاب ہے۔ بوطیقا میں ارسطو نے نقل، فطرت، شاعری کی اصل، شاعری کی اقسام، المیہ کے اصول وغیرہ پر بحث کی ہے اور شاعری کا ایک آفاقی نظریہ پیش کیا ہے۔ ’’نقل‘‘ جمالیات کی ایک بنیادی اصطلاح ہے۔ ارسطو اس لفظ کا اطلاق شاعری پر کرتا ہے۔ پروفیسر بوچر کے الفاظ میں ارسطو کے ہاں نقل کا مطلب ہے حقیقی خیال کے مطابق تخلیق کرنا اور خیال کے معنی ہیں اشیا کی اصل جو عالم مثال میں موجود ہے، جس کی ناقص نقلیں اس دنیا میں نظرآتی ہیں۔ عالم حواس کی ہر شے عالم مثال کی نقل ہے۔ دنیا کا پہلا معلوم شاعر...
Gelatin is derived from Collagen which is a natural protein in the animal’s skin and tissues like cows and pigs. So in this connection there is a doubt in its status that whether it is lawful (Halãl) or unlawful (Harãm). There are two different views about Gelatin. According to the 3rd Fiqhi Conference held in Makka al Mukarma that such Gelatin which has been derived from the Pig tissues and skin is not permissible. On the other side Islamic Fiqha Academy, India has decided that all kind of Gelatin is permissible because of Istihala i.e. Its change from one thing into another thing in the light of the views of Imam Muhammad bin Hassan Alshibani in Raddul Muhtar Aala Durrul Mukhtar.
Investigations of gene expression patterns of a gene or set of different genes in eukaryotic cells at particular stage or conditions require appropriate set of internal controls for accurate quantitative data analysis. These internal controls or reference genes are essential for ensuring the cell viability. Housekeeping gene/s (HKG) has been commonly used as reference genes for the normalization of gene expression data due to their presumed stable and constitutive expression in living organisms. The selection of appropriate internal control gene is a critical step for the gene expression studies by qRT-PCR due to variability in the stable expression of candidate HKGs in different organisms, different organs/tissues of same organism or most imporatantly due to different experimental conditions or environmental conditions for the same sample. In this study, we challenged the consensual thinking that all HKGs are reliable controls for expression studies through detailed investigation of set of potential reference genes suitable for gene expression analysis of Ficus carica (dicot), Chenopodium album (dicot) and Mentha spicata (dicot) after treating with different experimental conditions of abiotic stresses. We identified and sequenced three isoforms of actin (actin, β-actin and actin α), two isoforms of tubulin gene (β-tubulin and β-tubulin-1), GAPDH, EFα1, ubiquitin and 18S rRNA genes from these three plants and checked their validity as good internal control genes. Gene specific primers of above mentioned genes were designed from the conserved regions of similar genes from other plants to amplify genomic DNA followed by sequencing. After confirmation of right products from each plant using several bioinformatics tools, all of these sequences were submitted to genebank. Initially the similarities of these newly isolated genes with other known homologues or orthologues were determined. Alignments and phylogenetic trees have shown high levels of conservation in the genes among diverse set of selected monocots and dicots, as expected. In order to validate the utility of these newly isolated genes as internal controls, we treated the above mentioned plants with various abiotic stresses including heat, metal, cold, drought, salt and growth hormones. The respective transcripts were amplified and sequenced for further confirmations. We could hardly observed significant difference in the expression of these genes by semi- quantitative RT-PCR as expected. For quantitative validations, we used all of the above candidate internal controls to study the differences in their expression by relative quantitative real-time to validate the best gene or set of genes for the normalization of target genes in these plants. Different reference genes of C. album, F. carica and M. spicata showed somewhat variable expression under one or the other type of abiotic stress treatment suggesting; 1) Different experimental conditions can variably affect the stability of these internal control genes. 2) β-tub, EFα1, Ubq and Act-α were identified as the most stable genes under cold, drought, heat, metal and salt stress in C. album. In F. carica, 18S rRNA, β-tub and Act were three stable genes under cold, drought, salt and growth hormone stress. Similarly, GAPDH, β-tub, β-tub-1 and EFα1 were the stable internal control genes under drought, cold, heat and salt stress in M. spicata respectively. Furthermore, the gene stability index was generated for these selected HKGs which facilitated the selection of more than one internal control gene for further verifications of gene expression studies of these or any other related plant species.