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Home > Studies on Germination Ecology, Phytotoxic Effects and Control of Rhynchosia Capitata Roth Dc in Mungbean.

Studies on Germination Ecology, Phytotoxic Effects and Control of Rhynchosia Capitata Roth Dc in Mungbean.

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Author

Hafiz Haider Ali

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2013

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/1223

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727293062

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Studies on dormancy, ecology, phytotoxic effects, competition and chemical control of Rhynchosia capitata (Roth) DC in mungbean (Vigna radiata (L.) Wilczek) were investigated in the laboratory experiments at Department of Agronomy, University of Agriculture, Faisalabad and under farmer’s field conditions. In laboratory experiments different treatments for breaking dormancy of R. capitata seeds were evaluated. Seeds were soaked in thiourea, KNO 3 , HCl, HNO 3 and H 2 SO 4 as well as scarified mechanically (sand paper). Results indicated that seeds of R. capitata show signs of physical dormancy that is mainly because of impermeability of their coat. Mechanical scarification, acid scarification (soaking of seeds in H 2 SO 4 for 60 and 80 and in HCl for 12 and 15 h) were very effective in breaking dormancy and promoting germination. Seed soaking in HNO 3 for 1 to 5 days showed little effect, while, various concentrations of thiourea and KNO 3 were ineffective in breaking seed dormancy of R. capitata. Germination response of R. capitata to environmental factors such as temperature, salt stress, drought stress, pH, light and seeding depth were also studied. Germination increased as the temperature increased from 25 o C and considerably reduced at 45 o C. Germination of R. capitata seeds was not influenced by presence or absence of light. Increase in salt stress, moisture stress and seed burial depth significantly decreased the seed germination of R. capitata. Seeds of R. capitata had ability to germinate over a wide range of pH (5-10). In seed burial trial, maximum seedling emergence of 93 % was recorded at 2 cm depth, and seedlings failed to emerge from a depth of 12 cm. In third laboratory experiment, allelopathic influence of R. capitata on germination and seedling growth of mungbean along with detection of the phytotoxic materials liable for this action were studied. Aqueous extracts of root, shoot, leaf, fruit and whole plant (5%) adversely affected germination and seedling growth of mungbean, but higher inhibition was seen with R. capitata leaf water extract. A linear decrease in the germination characteristics of mungbean was observed with the decrease in the concentration of leaf extract from 5% to 1%. The soil incorporated residues (1-4% w/w) of R. capitata stimulated the development of root and hypocotyl at low concentrations and inhibited their development at elevated concentrations. Rhynchosia capitata soil incorporated residues (4% w/w) significantly reduced the seedling vigour index of mungbean in addition to its significant effect on total germination. A noteworthy amount of water soluble phenolic acids were found in extracts of different plant parts of R. capitata. Total phenolic acids were greater in leaf extract compared to that of stem, fruit or root extracts. Two phenolic acids, vanillic acid and 4-(hydroxymethyl) benzoic acid were found in R. capitata leaf extract. Effect of different weed crop competition periods i.e. full season competition, weed crop competition for 3, 4, 5, 6, 7 weeks and zero competition were studied under field conditions on mungbean. The results showed that full season weed competition produced highest weed fresh and dry weight, maximum NPK contents (%) and NPK uptake, highest Fe, Mn, Na, Zn, Ca, Cu and Mg contents and micronutrients uptake by R. capitata in both the years of study. The maximum plant height, number of pods per plant, grain number per pod and 1000-grain weight of mungbean was recorded in weed free plots. Increase in competition period decreased above parameters of mungbean significantly. Increase in R. capitata competition period decreased the mungbean grain yield significantly. In 2011, the weed-free plots gave the highest grain yield of 1688.6 kg ha -1 followed by competition 3 weeks after planting with 1582.0 kg ha -1 of seed xxiyield. The full season R. capitata competition decreased the grain yield to 869.3 kg ha -1 . Similar trend was also observed during 2012. In second field experiment, the efficacy of herbicides namely pendimethalin + prometryn @ 875 g, 700 g and 525 g a.i ha -1 , S- metolachlor @ 1440 g a.i ha -1 and pendimethalin @ 825 g a.i ha -1 in controlling R. capitata was evaluated. All doses of the herbicides suppressed the dry biomass of R. capitata from 60 to 78% in 2010 and 2011. Pendimethalin+prometryn @ 875g a.i. ha -1 , recorded (74% in 2010 and 78% in 2011) maximum reduction in total weed dry weight. Among herbicide treatments, maximum grain yield was recorded with pendimethalin+prometryn @ 875 g a.i. ha -1 in both the years. Pendimethalin + prometryn @ 875 g a.i. ha -1 proved best treatments for effective control of R. capitata in mungbean and to get maximum economic benefits.
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تعقبات ابن العراقي واستدراكاته في تحفة التحصيل على العلائي في جامع التحصيل

The research reveals significant insights cited by Ibn Al ‘Irāqī in his book "Toḥfah Al Taḥṣīl" on the illusions of Al-‘Alā'ī in his book "Jāmi Al Taḥṣīl". It highlights the scientific value of those illusions that Ibn Al-‘Irāqī pointed out. Several of them are related to narrators of hadith and their issues of hearing from their sheikhs. Many of those illusions are related to the chain narrators (isnad), the main text of the report (matn), or their position in the books of sunnah. There are no previous studies on this subject. I put the sequential insights I revel under headlines through which one can realize the illusions that Al-Ala'i fell in. I conclude with the perceptions that both Ibn Al-‘Iraqī and Al-‘Alā’ī have shared. I don’t mention my opinion after each insight for Ibn Al-‘Iraqī; however, my silence is an approval to what he said. When I went against him or it was important to mention any comment or information, I openly said my opinion and explained the reasons for my opposition. Some of the research findings are: Al-Ala'i ignores mentioning the narrator's gap (irsal), although Al-Mizzī mentions it in his book "Tahdhīb", or the scholars mention it before both of them.  He describes the narrator having a gap (irsāl) and attributes it to Al-Mizzī.  He added notes like "he didn't encounter him" and formulated expressions that weren’t mentioned by neither Al Dhahabī nor Al-Mizzī. He references a Ḥadīth to a book that it is not included in.

Molecular Characterization of Local Strains of Plasmodium Vivax Based on Pvcsp Gene

Plasmodium vivax is the most prevalent species and always remains a major cause of mortality and morbidity in endemic areas, accounting for at least 65 ? 70% of all cases happening in Pakistan. However, Pakistan is deadly lacking information about the structure, role in drug resistance and population of P.vivax species. To get knowledge about the parasitic origin and diversity of P.vivax, the study of its population genetics is necessary. The PvCsp gene is considered as highly polymorphic in nature and much important for the survival of P.vivax under severe pressures of the environment in different areas. Also for the development of vaccine, this gene has been identified as a potential candidate. The present study focused at the investigation of the structure of the P. vivax populace through the diversity of PvCsp gene. To get the nucleotides sequences of the PvCsp gene, DNA extraction, PCR and Sanger sequencing of P.vivaxsamples collected from local areas of Rawalpindi and Islamabad.The sequences were aligned and analyzed by using Geneious prime and Bio Edit software. The phylogenetic trees were constructed using ML tree and neighbor joining tree methods. Many nucleotide differences, repeats, deletions were found at different places in the PvCsp.The study provided a lot of information about the variations or mutations in the PvCsp gene of our localP.vivaxstrains. It is concluded that local P. vivax strains of federal area of Pakistan depicted significant variations as compared to the Reference sequences. Therefore\, it is suggested that prevailing P. vivax strains of federal areas of Pakistan might be categorized as a different strain which need further investigations.