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Studies on the Development of a Consortium of Fungi and Bacteria for Efficient Composting

Thesis Info

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Author

Noreen, Nayara

Program

PhD

Institute

University of Karachi

City

Karachi

Province

Sindh

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Subject

Botany

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/9822/1/Nayara_Noreen_Botany_2016_UOK_30.11.2016.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727336754

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Composting is the microbial degradation of organic waste material into a useful product that can provide nutrient to plants. Composts containing biocontrol agents can be useful in reducing the use of chemical fertilizer and pesticides since these composts not only increase the plant growth but also provide better suppression in root colonization by soil borne pathogens. During the present studies, microorganisms isolated from various composts were evaluated for their enzymatic and biocontrol activities in order to develop consortia of microorganisms that can reduce the time required for composting, and also provide protection to roots against soil-borne pathogens. A total number of 15 bacteria including six mesophilic, five thermotolerant and four thermophilic, and 44 fungi including 13 mesophilic, 11 thermotolerant and 20 thermophilic species isolated from cow dung, goat pellet, poultry manure and plant debris. Thermophilic fungi viz., Annelophora africana, Conidiobolus thermophilus and Haplotrichum croceum have been reported for the first time from Pakistan. The highest population of thermophilic microorganisms was observed in cow dung followed by goat pellet poultry manure and plant debris. The highest population of thermotolerant microorganisms was recorded from goat pellet followed by poultry manure, cow dung and plant debris. Similarly, the x highest population of mesophilic microorganisms was in plant debris followed by poultry manure, goat pellet and cow dung. The growth of thermophilic fungi was better on yeast starch agar as compared to potato sucrose agar medium. Similarly, the thermotolerant fungi showed better growth on PSA. Among the mesophilic fungi, Aspergillus flavus, Penicillium dipodomyis, Stachybotrys chartarum and Syncephalastrum racemosum showed better growth on YSA medium, PSA was more suitable for the remaining mesophilic fungi. During the present study, 33 fungi and 7 bacteria exhibited amylase activity. Of these, no previous report on amylase activity of Mucor fragilis, Aspergillus floccosus, Aspergillus nidulans (Teleomorph) and Aspergillus rugulosus is available. Similarly, 40 fungi and 6 bacteria showed cellulase activity that included Annelophora africana, Haplotrichum croceum, Aspergillus floccosus, Trichoderma virens and Micrococcus varians as now sources for cullulase enzymes. A total of 17 fungi and 7 bacteria showed chitinase activity and 20 fungi and 5 bacteria showed pectinase activity. No previous report on pectinase activity of Aspergillus rugulosus and Micrococcus varians is available. Furthermore 27 fungi and 6 bacteria showed protease activity. xi In vitro compatibility interactions showed that Acremonium thermophilum, Annelophora africana and Haplotrichum croceum were compatible with 10 fungi. Similarly, Bacillus cerreus and B. megaterium were compatible with 11 different fungi. Individual inoculations of the 44 fungi and15 bacteria into the composting materials showed that rate of degradation of grass clipping was the highest, followed by fruit waste, leave and wheat straw in descending order. The mesophilic microorganisms showed no composting at 50°C but composting activity was very good in thermopole boxes. The thermotolerant microorganisms were less effective at 50°C but in thermopole boxes these activities was very good. The thermophilic microorganisms were equally effective both at 50°C and in thermopole boxes. During single inoculations, the efficient thermophilic microorganisms took 7-8 days to completely degrade the grass clippings into compost. The time taken by the efficient thermotolerant and mesophilic microorganisms was 8 and 12 days, respectively. Combined use of two microorganisms degraded the grass clippings within five to eight days. Combinations of Isaria fumosorosea with Thermomyces lanuginosus, and Bacillus licheniformis with Aureobasidium pullulans were the most efficient. Based on the compatibility between the different microorganisms, three consortia with 17 members in each, developed to evaluate their xii composting ability. Consortium-1 showed the most efficient composting followed by consortia 2 and 3. The populations of the microorganisms were higher in compost developed by consortium-1 followed by consortia 2 and 3. The population densities in all the composts increased after four days whereas after 14 days, the populations of the microorganisms reduced and no bad smell was observed. It indicated the complete degradation of grass clippings. In case of the control, the bad smell continued even after 14 days, that indicates slow degradation. During pit composting, the initial average temperature of the composting material was 20°C. Only the mesophilic microbes isolated during the initial three weeks. The thermotolerant microbes thrived during 4th and 5th weeks. Thermophilic microbes grew from 6th to 8th week and finally disappeared. The peak heating phases (50oC) was at 8th week of composting, and during phase, the highest numbers of thermophilic microorganisms were isolated. Thereafter, the temperature gradually reduced to 40°C by the 9th and 10th weeks; the thermotolerant microbes reappear and later mesophilic microbes re-colonized the compost at 11th and 12th weeks when temperature reduced to 28°C. In pot and field experiments, composts developed by the consortia of microorganisms increased plant growth and reduced root colonization by soil borne pathogen viz., Fusarium solani, Macrophomina phaseolina, xiii Pythium aphanidermatum, Rhizoctonia solani and Sclerotium rolfsii. Use of consortia composts @ 10% w/w gave the highest increase in growth parameters followed by 5% and 1% treatments. Compost developed by consortium-1 was more effective as compared to consortia 2 and 3; consortium-3 compost being the least effective. The efficacy of the composts was greater in pot experiment as compared to field experiments. Plants treated with consortium-1 compost at 5% and 10% showed flowering within one month. Plants treated with consortia 2 and 3 composts flowered after one month only in 10% treatments. No flowering observed in one month old control plants. This early flowering occurred in pot experiments but not in field experiments. In field experiments, soil amendment with consortia composts showed persistence of the efficacy. Mungbean plants grown in the same micro plots without further addition of the composts showed a slight reduction in plant growth promotion and suppression in root colonization by soil-borne pathogens. Planting for the third time without adding consortia composts resulted in further reduction in the efficacy of the compost. However, the plant growth and suppression in root colonization was still better than that in control plants.
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راکی ولسن کا’’مینار محبت‘‘

راکی ولسن کا’’مینار محبت‘‘

کائنات میں اشرف المخلوقات ہونے کا شرف صرف انسان کو حاصل ہے تمام مخلوقات میں انسان سب سے زیادہ با اختیار بھی ہے۔احساسات و جذبات بھی سب سے زیادہ انسان ہی رکھتا ہے۔اسی لیے وہ ازل سے اپنے خیالات کا اظہار کرتا آیا ہے۔کبھی آرٹ ،تصاویر،لوک داستانیں اور کبھی شعر وادب کی صورت زبان کا اظہار بہترین ذریعہ ہے۔لفظوں کو ایک خاص انداز میں اپنے خیالات کے اظہار لے لیے استعمال کرنا شاعری ہے۔راکی ولسن نے بھی اپنے خیالات کے اظہار کے لیے خوبصورت الفاظ کا استعمال کیا ہے۔اس لیے اسکی شاعری و خود جذبات کے تحت وجود میں آتی ہے۔

راکی نے رشتو ں ناتوں میں بٹے ہوئے انسان کو اس کی خوشیوں،غموں،امنگوں، آرزوؤں،اْمیدوں،نا امیدوں،رویوں اور ولولوں کو اپنا موضوع بنایا ہے۔اسکی بے ساختہ شاعری فطرت انسانی کی عکاس ہے،وہ موضوعاتی شاعری پر بھی طبع آزمائی کرتے دکھائی دیتے ہیں۔انکی شاعری میں متاثر کرنے کی زبردست صلاحیت موجود ہے۔یہ صلاحیت صدق دل اور خلوص نیت کے بغیر حاصل نہیں ہوتی۔یہ خلوص نیت ان کے مجموعہ کلام ’’مینار محبت‘‘ میں محبت بانٹتے ہوئے دکھائی دیتا ہے۔آپ کے اس مجموعہ کلام اجمالی جائزہ کچھ اس طرح سے ہے۔

اگرچہ شاعر نے شاعری کی روایت سے ہٹ کر اس کتا کو شروع کیا ہے۔مگر اس کے باوجود وہ اپنے پروردگار کو بھولے نہیں۔کہتے ہیں کہ ہر شے میں اس ذات باری تعالیٰ کا جلوہ موجود ہے اسی لیے تو کائنات کی ہر شے اس کی خوبصورتی کو دیکھنا چاہتی ہے،آج راکی صاحب جو کچھ بھی ہیں وہ سب اس ذات کی کرم نوازی کی وجہ سے ممکن ہوا ہے:

حسن فطرت کے مظہر ہے تیری شان وجیہہ

تیری دید کی طالب ہر آنکھ شیدائی ہے

المفتي: شروطه وآدابه

The duty of issuance Islamic legal verdict is a great job because the Mufti is the successor of the Prophets of Allah. He explains the commands of Allah — permissible and prohibited acts—and stops the disputes among the followers. The focal aspects of this research paper are the questions: What are the required conditions to be a reliable mufti? What are the points of agreement and differences among the four major schools of jurisprudence — Hanafi, Maliki, Shafa’i and Hanbali? The most important area of this paper is the question: What are the protocols and etiquettes of the procedure of issuance of a fatwa, the legal verdict, in our contemporary societies. In the first part of this paper, the analytical evaluation of the arguments presented by prominent jurists of the four major schools of Islamic Jurisprudence. This part suggests some points to reset the preferences because it is the need of time. The second part of the paper opines a number of suggestions to improve the manners, etiquettes and protocols of the procedure on part of a mufti. A mufti, being a representative of the seat of the Prophet (peace and blessings of Allah be upon him), is not only responsible to Allah Almighty but also to wellbeing, security, and peace among the members of our society.

Isolation and Biochemical Characterization of Efficient Strains of Rhizobium and Azospirillum from Wheat and Maize under Water Stress

Water stress is one of the most important environmental constraints that limit survival and productivity of staple crops like wheat and maize particularly in arid and semi-arid regions. Plant growth promoting Rhizobacteria (Rhizobium and Azospirillum) are beneficial bacteria present in soil and forming associations with roots of plants. Present study was aimed at isolation and biochemical characterization of Rhizobium and Azospirillum strains from roots and rhizospheric soil of wheat and maize (at tillering and anthesis stage) growing under different moisture regimes in the field and under induced water stress in pots. The survival efficiency as measured by log cfu/g, carbon/nitrogen utilization pattern determined by QTS (Quick Test System), random amplification of polymorphic DNA analyses, phytohormone production and 16sRNA sequence analysis were done for isolated strains. The efficiency of isolated strains to promote plant growth and development was tested under induced water stress conditions in reinoculation studies. The survival efficiency of Rhizobium and Azospirillum isolates from plants of irrigated field (Experiment I) and those from well watered pots (Experiment II) was higher as compared to that of Rhizobium and Azospirillum isolates from roots and rhizosphere samples of arid (8 % soil moisture) and semiarid field ( 14 % soil moisture)plants and waters stressed plants( 8 % soil moisture) in pots. There was no significant difference in the survival efficiency between root and rhizospheric soil isolates. The effect of low soil moisture on colony forming unit was more pronounced at anthesis stage of plant growth as compared to tillering stage. The value of cfu/g was higher for isolates from maize as compared to that of wheat. On the basis of carbon/nitrogen utilization patterns the Rhizobium and Azospirillum isolates from wheat and maize field were placed in three different groups depending upon the different moisture regimes from where these strains were isolated. xviThe UPGMA (Unweighted Pair Group Method with Arithmetic Means) cluster analysis based on Random Amplification of Polymorphic DNA (RAPD) tests grouped Rhizobium and Azospirillum isolates in two. The results revealed that QTS test can not solely be implicated to fully characterize microbes and for better understanding of phylogenetic relationship, molecular characterization e.g. RAPD-PCR is preferred. The strains isolated from plants growing at semi arid regions in field and under induced water stressed in pots at tillering stage exhibited close genetic relatedness to the strains isolated from plants growing under irrigated field conditions and well watered conditions in pots respectively. While the strains isolated from field plants growing at semi arid region at anthesis stage were found to bear close genetic relatedness with the strains isolated from plants growing in arid regions in field. Reinoculation experiments with both field and pot isolates revealed that the strains of Rhizobium and Azospirillum isolated from well watered plants grown in field or pot have less effect on plant growth of either chickpea or wheat used as host respectively, whereas Rhizobium and Azospirillum isolated from arid fields and plants growing under water stressed pot conditions performed better exposed to induced water stress. The amount of IAA, GA, ABA and trans zeatin riboside produced in the presence of tryptophan was considerably higher as compared to medium from which tryptophan was omitted. Moisture stress at all levels (14 to 8% soil moisture) decreased the production of IAA, GA and trans zeatin riboside but ABA production was increased. These isolates further showed bacteriocin production. Degree of association of microbes appears to depend on developmental stage and on its root architecture of host plants. Rhizobium and Azospirillum isolated from arid field and induced water stress in pots can be used as inocula to promote growth and yield of plants grown in moisture deficit soil.