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Study of Metabolic Syndrome Susceptibility Genes Through Genetic Screening

Thesis Info

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Author

Fiaz, Muhammad

Program

PhD

Institute

Pir Mehr Ali Shah Arid Agriculture University

City

Rawalpindi

Province

Punjab

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Subject

Biochemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/9788/1/Muhammad_Fiaz_Biochemistry_2016_HSR_PMAS_22.08.2016.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727379629

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The metabolic syndrome (MetS) is a combination of risk factors which increases the risk of developing cardiovascular diseases (CVDs) and type 2 diabetes (T2D). The major risk factors of MetS are central obesity, hyperglycemia, dyslipidemia, hypertension The CVDs and T2D are the main cause of death worldwide and especially in South Asian countries. An increase prevalence of MetS in Pakistan is an alarming condition. The present study was designed to find out the prevalence of the MetS, assessment of risk phenotypes responsible for MetS and genetic susceptibility of the MetS in the Pakistani population. The questionnaire based physical data and whole blood samples were collected by visiting the Out Patients Departments (OPDs) of different hospitals of Rawalpindi and Islamabad. Biochemical analysis was performed following the standard protocols. Total 9 genes including FTO, LPL, CETP, GCKR, APOA5, ADBR2, FADS2, ADIPOQ, CD36 and 32 SNPs were selected for study. The genotyping of all the SNPs was performed using MassARRAY iPLEX Gold Technology in the Genome Research Center, The University of Hong Kong, Hong Kong. The Statistical analysis of the data was analyzed by using Plink and SPSS softwares. The overall prevalence of MetS was 46% and the males and subjects having advanced age were found to have more risk of developing MetS. Out of 9 genes, the SNPs rs1421085, 1558902 of FTO, rs662799 of APOA5 and rs780094 of GCKR were found to have association with MetS in our population
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۴ناول رقص نامہ میں سندھی عناصر اور سندھی سماج

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کومل شہزادی،پی ایچ ڈی اسکالر

سندھ کی سرزمین دنیا کی واحد دھرتی ہے جہاں پہلے انسان حضرت آدم علیہ السلام کے قدم پڑے اور ان کی اولاد کا جنم و دفن ہوا ، ھابیل بن آدم دادو ضلع کے کا چھو کے علائقے کا سبوجبل ( پہاڑ) میں مدفون ہے حضرت محمد ﷺ کو بھی سندھ کی سر زمین سے بیحد پیار و محبت تھی جن کے حوالہ جات آگے آئیں گے۔قرآن پاک میں سندھی قوم کا ذکر ہوا ہے ،کتنے ہی سائنس اور تاریخی حوالہ جات ذاتی تجربات و مشاہدات کی بنیاد پر ثابت کرنے کی کوشش کی ہے کہ سندھ ہی انسانی و حیوانی ارتقا کا جنم بھومی ہے ۔جب تمام دنیا کی دھرتی برف کے تہوں سے برف پوش تھی تب سندھ کی دھرتی ہی وہ واحد خطہ ارض تھا جہاں برف نہیں ہوتی تھی  اور گرم رہنے والا خطہ تھا ،جہاں انسان اور حیوان موجود تھے ،شونکہ سردی میں انسان اور جاندار کا زندہ رہنا ممکن ہی نہیں ہوتا اور گرم علاقہ صرف سندھ کی سرزمین ہے سائنس  بھی اس بات کی تصدیق کرتی ہے ۔سندھ کی تاریخ عربوں کھربوں برس پرانی ہے ، سندھ کا خطہ تاریخ کے ان خطوں میں شمار کیا جاتا ہے جہاں مسلسل آبادی و خوشخالی رہی ہے ۔سندھ کی سرزمین پرموہن جو دڑو ، ہٹرپہ کی عظیم تہذیبوں نے جنم لیا جن کا مرکز موہن دڑو رہا ہے ،موہن جودڑو کی نسبت سے علما سے ملاقاتوں میں یہ بات سامنے آئی ہے  کہ عاد قبیلے کا جد امجد موھن نامی ایک شخص تھا ،جنہوں نے رنی کوٹ کا قلعہ تعمیر کروایا ۔سر زمین سندھ کو اپنے مورث اعلیٰ حضرت سندھ سے منسوب سات دریاؤں سے بھی منسوب کیا جاتا ہے ۔یہ سات...

الدعوة الاسلامية دعوة عالمية

Allah almighty has created Human beings, the angels, and jinn for His worship alone. To guide the human beings to the right path, Allah almighty has sent many messengers to all nations and pieces of lands. Hazrat Adam (A. S.) was the first human being as well as the first Prophet of Allah on the Earth, while the Prophet Muhammad (PBUH) was the last of all in this chain. There will be no prophet after him. Now, it is the duty of every Muslims to carry and spread this universal message to the whole humanity. In this context, the message of Islam is universal. This article deals with this issue through giving proofs from the Holy Qur'an and Sunnah.

Dynamics of Bacterial Assisted Phytoremediation of Soil Contaminated by Quinolone Antibiotics

Recently there has been growing concern about public health and ecosystems due to the presence of trace quantities of quinolone antibiotics in the environment. These antibiotics enter the environment through irrigation by contaminated waste water, particularly in the areas that are arid or semi-arid. Another route of these antibiotics is the application of sludge and manure containing antibiotics as soil amendments on agricultural land. Once they enter the environment they can cause ecotoxic effect. In the current study, the concentration of ciprofloxacin, enrofloxacin, and levofloxacin in waste water and sludge were relatively higher than reported in many countries. To degrade the aforementioned antibiotics in agricultural soil, eight bacterial strains were isolated and characterized. The isolated bacterial strains possess multiple antibiotic resistance and degraded ciprofloxacin by whole cell activity. No DNA transformation were observed when naked DNA of the isolated bacterial strain were exposed to indigenous soil microbes. The isolated bacterial strains survived well under aerobic conditions in soil treated with ciprofloxacin at the concentration of 5 mg kg-1, 50 mg kg-1 and 100 mg kg-1. The quinolone antibiotics did not affect germination of wheat even at 400 mg L-1 concentration but the young wheat seedlings were sensitive to the antibiotics at relatively low concentrations (5 mg L-1). The toxicity of the quinolone antibiotics ciprofloxacin (CIP), enrofloxacin (ENR), levofloxacin (LEV), and a mixture (MIX) of the same were tested in a greenhouse study with wheat for 20 days. Antioxidants, which play a vital role in detoxifying reactive oxygen species (ROS) during stress, were xxii also studied. The highest antibiotic concentrations, 100 and 300 mg L-1, significantly decreased plant growth and caused oxidative stress. The quinolone antibiotics ciprofloxacin (CIP), enrofloxacin (ENR), levofloxacin (LEV), and a mixture (MIX) in agricultural soil were readily sorbed when tested in agricultural soil. The antibiotics showed high sorption coefficient (Kd) values at low concentration and low Kd values at high concentrations, which means sorption was concentration dependent and time for sorption decreased with increasing concentrations. In both the individual and MIX treatment the order of sorption was CIP > ENR > LEV and for desorption it was LEV > ENR > CIP. In a bacterial assisted phytoremediation study, wheat was grown in greenhouse conditions in soil supplemented with CIP, ENR, and LEV at 5, 50 and 100 mg kg-1 in individual and MIX treatments. CIP, ENR and LEV accumulated in plant roots and shoots from soil and more accumulation was observed in roots. The order of accumulation in plant parts was LEV > ENR > CIP both in individual and MIX treatments. The pots having only plants enhanced the recovery of antibiotics from soil compared to those where there were only bacteria but greater degradation was observed where plants were grown in the presence of bacteria. CIP, ENR, and LEV were completely degraded in individual treatments at 5 and 50 mg kg-1 level in the presence of plants and bacteria after 30 days compared to the control. In the MIX treatment all three antibiotics were less degraded compared to individual treatments in the presence of plants and bacteria after 30 days because of the higher concentration of each antibiotic in the pots.