Cockroaches are one of the most important pests in urban communities and are risky for human health because they play an important role in transmitting different diseases either mechanically or occasionally biologically. Environmental and sanitary conditions associated with demographic and socio-economic settings of an area could contribute to the prevalence of disease pathogens carried by cockroaches. The present study was aimed to determine taxonomical identification and diversity of cockroaches in hospitals and houses in Lahore, Pakistan and to evaluate the role of cockroaches in transmission of important disease pathogens by using microbial screening of outer surface and digestive tract. Resistance and susceptibility to antimicrobials and disinfectants was also investigated, followed by quantitative and qualitative analysis of total bacterial protein. Four species of cockroaches (P. americana, B. germanica, B. orientalis and B. lateralis) were identified in this study. B. germanica was found to be the most dominant indoors species with highest diversity indices followed by P. americana. Species diversity was highest in July–September, 2013 with highest Simpson index of diversity and Shannon index as well. Population index of B. germanica for hospitals was double than that of residential areas. Houses and hospitals were highly infested with P. americana and B. germanica as compared to offices, shopping malls/ departmental stores and universities. Whereas B. orientalis was commonly found in houses, institutes/universities followed by hospitals, while B. lateralis was common in institutes/ universities, houses and offices with basements and gardens. P. americana was found higher in all trimesters (mean 1179.50 + 351.77) and the analysis of variance revealed a significant difference between the groups (F(3,4)=9.65, P=0.005). Significant correlation was found among changes in temperature and abundance of P. americana in study area (Pearson correlation, r= 0.904, P= 0.04). Similarly temperature showed positive correlation with population of B. germanica (r= 0.958, P= 0.021) and B. orientalis (r= 0.987, P= 0.007). During the study, all cockroaches were found to be infected with at least one bacterium. The most common bacterium isolated from external surface of cockroaches was E. coli (10.31%), followed by S. aureus (10.09%), while P. aeruginosa (19.96%) was isolated from internal gut tract of cockroaches, followed by P. vulgaris (16.08%). Among hospitals the highest in external bacterial infection was observed on P. americana in Punjab Institute of Cardiology (PIC) (75.6%) while ii highest internal bacterial infection was observed in Sheikh Zaid Hospital (SZH) (30.8%). Same trend was observed for bacterial isolation from B. germanica in hospitals. Among houses highest external bacterial infection for P. americana (55.9%) and B. germanica (52%) was observed in samples collected from Shalamar- II. However, highest internal contamination (25.8%) for P. americana was observed in Mughalpura-I locality while highest internal infection (28.8%) for B. germanica was Model town-3 houses. Jaccard’s index of similarity was highest (0.3125) in houses while Bray-Curtis index of dissimilarity was highest for hospital (0.2174). The highest Shannon-Wiener’s diversity index value was found for Punjab Institute of Cardiology (2.610632). All cockroaches had also carried one or more species of medically important mould on their external surface. In this study the most common mold isolated and identified from First Fungal Culture Bank of Pakistan were A. oryzae (84%) and A. flavus (75%) while least common was G. candidum (22%). Cockroaches collected from Shadman-1, Johar town-1 and Shalamar-2 showed relatively high fungal prevalence. P. americana harboured more parasites as compared to B. germanica in both environment. E. coli protozoan was found as the most prevalent followed by E. vermiculari however, A, lumbricoides were least prevalent in hospitals and houses. Simpson Diversity index value of parasitic contaminants isolated from B. germanica collected from houses was 0.92133 and 0.91827 for hospitals. The Shannon-Weiner diversity index calculated value was found highest for P. americana at both sites houses and hospitals as 2.554291 and 2.536765 respectively, which predicted that the rate of parasitic contaminants of both species was not even. Both experimental sites were not significantly different in carriage of parasitic contaminants on cockroaches (F (1,6) =1.795, P= 0.229). Resistance to amoxicillin was found 100% for both gram negative and gram positive isolates followed by cephradine and tetracycline respectively. E. coli was observed as resistant to 3 out of 5 antibiotics (AML, CE, TE) followed by P. aeruginosa that showed resistance to amoxicillin and tetracycline. Germ Kill Vantocil FHC was found more effective bactericide than Germ kill Vantocil in current study. Germ kill Vantocil FHC exhibit highest inhibition zone diameter mean (27+11.575) for 12.5% diluton and 14+13.856 at 50% dilution. Similarly RIZD was 81.81% for 12.5%, dilution respectively. Protein bands of control group were compared with resistant bacterial samples and all protein bands are lying between 236216.2 kDa – 10000 kDa.
ارادھنا (کرونا وائرس کے تناظرمیں) اے خالق عصر۔۔۔اے مالک ’’کن‘‘۔۔۔! مسجدوں کے دروازے بند ہیں مندروں میں ہُو کا عالم ہے آنسوئوں سے بھیگی صدائوں میں! قاتل ہوائوں میں! بچے گلیوں میں نکلتے نہیں۔۔۔کرفیو کا نفاذ بھی نہیں اہلِ زباں۔۔۔حسن بیاں کے پھول کھلتے نہیں اے خالق ارض و سما۔۔۔! عنکبوتی تاروں کی طرح وبا کی موجودگی میں! اسلوب کنعان میں ڈوبی جوانیاں، موج زلیخا کی دلفریب روانیاں۔۔۔! روایاتِ اذیت سے ڈرتے ہوئے گھروں سے نکلتی نہیں اے ازل کے مالک۔۔۔اے ابد کے خالق۔۔۔! یہ دنیا تیری ہی بنائی ہوئی ہے جس کے سر پر وبا موت بن کے چھائی ہے نشاط روح، سرور ازل ، وعدئہ اول کو یاد کرتے ہوئے! شبنمی گلابوں پر اُداسی چھائی ہوئی ہے کوئلیں ، فاختائیں اور عندلیبانِ چمن۔۔۔! نہ جانے کن گمنام جزیروں کی طرف نکل گئی ہیں اے رب دو جہاں۔۔۔کچھ معلوم نہیں رحم فرما ۔۔۔رحم فرما ۔۔۔اے حافظ حفیظ رحم فرما بہلول مسکراتے ہوئے بولا۔۔۔ اچھا تم نثری نظم لکھتے ہو۔ عہدِحاضر کی اک معروف شخصیت جسے ریاض مجید کہتے ہیں۔ وہ اسے نثم کے نام سے یاد کرتا ہے۔ اس صنف کے لیے دونوں نام بہتر ہیں۔ لیکن میں ’’نثم‘‘ کو ذاتی طور پر پسند کرتا ہوں جو اس نام کو پسند نہیں کرتے ، نثری نظم پر بضد ہیں ۔مجھے اُن سے بھی اختلاف نہیں۔ ادبی دنیا میں اک مندائیت کی شکل میں فرقہ ہے جو اس صنف کو آج بھی نہیں مانتا۔ تم جانتے ہو۔۔۔مندائیت کیا ہے؟ میں نے نفی میں سر ہلاتے ہوئے جواب دیا، اور خاموش رہا۔ کیونکہ بہلول دلچسپ شخصیت کا مالک تھا اور میں اُسے صرف سننا چاہتا تھا ، کہ وہ کیا کہتا ہے۔ اُس نے میری طرف دیکھتے ہوئے ،بولنا شروع کیا۔۔۔! مندائی ایک فرقہ ہے۔ جو آدمؑ ،شیثؑ ، نوحؑ، سامؑ اور ادریسؑ کو مانتا ہے۔ یوحنا اصطباغی...
Qur’an and Prophetic traditions (Hadith) are the fundamental sources of Islam. Muslims believe that Qur’an is the word of God (Allah). Hadith (Prophet’s Sayings, actions and silent approval and disapproval for something) likewise is based on divine revelation. Qur’an affirms also this view: (God says) Your Companion (Muhammad) has neither gone astray nor has erred. Nor does He speak of (his own) desire. It is only a Revelation revealed. Al-Qur’an (53: 2-4). Allah Almighty Himself took the responsibility to guard His word (the Qur’an): (He says : ) verily, we, it is We Who have sent down the Dhikr (i.e. The Qur’an) and surely, We will got it (from corruption). (Al-Qur’an: 15: 9) on the contrary the responsibility to guard the prophetic traditions (Hadith) was put on the shoulders on the Muslim Ummah. The scholars of Islam (Ulamas) try their utmost to collect and save the Prophetic traditions and guard it from any alteration. To achieve this purpose, they introduced different hadith sciences to distinguished between the true and the fabricated hadith. The authentic Sunnah is contained within the vast body of Hadith literature. Different scholars have compiled the books which contain a large numbers of authentic Ahadith (Ahadith Sahiha), one of them is Imam Ibn e Khuzaima. In this article we will discuss the Imam Ibn e Khuzaima approach towards “Ahadith al Sahiha” in his book “Sahih Ibn e Khuzaima”.
Rice (Oryza sativa L.) is one of oldest domesticated specie and a major staple food of around 3 billion people in the world. An investigation to find out the genetic diversity among 113 cold tolerant rice accessions acquired from plant genetic resource institute (PGRI) origionally obtained from Northern Areas of Pakistan along with three check varieties (Super Basmati, IR6, and JP5) was conducted. Morpho-physiological data was recorded for 19 qualitative and 19 quantitative traits at different growth stages during the summer season 2014 at two locations. The data was analyzed by cluster analysis and principal component analyses (PCA). Results revealed significant level of variability for all the morphological traits. Maximum variation was observed in grain yield plant-1, (433.92 g) and plant height (311.28 cm). The accessions were grouped into seven clusters based on Euclidean dissimilarity index. PCA based on 19 quantitative traits showed that first five PCs accounted for 67.86% of the total variance, PCI explained 28.41%, PCII contributed 13.38% and PCIII accounted for 11.69% of total variation. Polymorphic genetic markers were also used to identify species relationship and to asses the degree of genetic diversity in the accessions. On the basis of absence and presence of 18 visible polypeptides, an electrophoretic profile was established for construction of dendrogram through UPGMA method. A significant amount of variation was revealed in Glutelin and Prolamin subunits, while low variation was observed in Albumin and Globulin subunits. It can be inferred from the present investigation that lower genetic diversity was xiv present in rice accessions based on total seed storage protein profile SDS-PAGE alone was found to be insufficient for reliable differentiation. High yielding 96 accessions were selected through morphological and biochemical characterization for the determination of allelic variability through microsatellite analysis using 36 SSR markers. An average of 3.5 alleles per locus were observed. Polymorphism Information Content (PIC) value with an average of 0.638 per locus was observed. From the results it is concluded that SSR markers were effective in detecting polymorphism in the accessions studied and other crops with high genetic similarity. Bacterial leaf blight is one of the most serious diseases of rice in world. For molecular characterization of these accessions against bacterial blight and fragrance, tightly linked STS markers were used to amplify the target fragment. Accessions were also analyzed for presence or absence of bacterial blight resistant genes (Xa4, xa5, xa13, Xa21 and fgr) through PCR amplification using STS primers. Out of 96 accessions 42 accessions were found to have Xa4 resistant gene, while 50 accessions were found to have xa13 resistant gene. Fifty two accessions were found to have xa5 resistant gene which is most prevalent gene in Northern areas germplasm, Xa21 resistance gene and fgr gene were found to be absent in the germplasm studied.