The research work was done to synthesize a series of novel chitosan/Curcumin based water dispersible polyurethanes with potential applications as antibacterial textile finishes. The synthesis were carried out in three steps, in first step the NCO end capped PU-prepolymer was formed through the reaction between Polyethylene glycol (PEG) (Mn=600), Dimethylolpropionic acid (DMPA) and Isophorone diisocyanate (IPDI)/ 4, 4’-dicyclohexyl methane (H12MDI). In second step the neutralization step was carried out by using Triethylamine (TEA) which results the formation of neutralized NCO terminated PUprepolymer, after that the last step chain extention was performed by the addition of chitosan/curcumin and followed the formation of dispersion by adding calculated amount of water. The proposed structure of chitosan/curcumin based WDPUs samples was confirmed by using FTIR technique. Using pad-dry-cure procedures, the synthesized chitosan/curcumin WDPUs samples were applied onto the different qualities of plain weave poly-cotton white, printed and dyed textile swatches. The textile properties of the treated and untreated textile swatches were measured, as color fastness, pilling resistance, tear and tensile strength. The antimicrobial activities of the plain weave poly-cotton printed and dyed textile swatches after application of chitosan/curcumin based WDPUs samples were also evaluated. The results showed that the chitosan/curcumin incorporation into PU backbone has markedly effect on the assets of treated textiles and also enhance the antibacterial activity of WDPUs. These synthesized chitosan/curcumin based WDPUs are ecofriendly antimicrobial finishes (using natural bioactive agents such as chitosan and curcumin) with potential applications on polyester/cotton textiles.