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Home > Synthesis and Characterization of Magnetic Nanoparticles Fe3o4, Co3o4 and Their Application in Urea Biosensing

Synthesis and Characterization of Magnetic Nanoparticles Fe3o4, Co3o4 and Their Application in Urea Biosensing

Thesis Info

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External Link

Author

Akbar Ali

Program

PhD

Institute

Riphah International University

City

Islamabad

Province

Islamabad

Country

Pakistan

Thesis Completing Year

2015

Thesis Completion Status

Completed

Subject

Physics

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/6881/1/Akbar_Ali_Physics_RIU_2015_10.02.2016.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727451010

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The present research work shows the fabrication of potentiometric urea Biosensor based on magnetic nanoparticles iron oxide (Fe3O4) and cobalt oxide (Co3O4) through simple, economical and reproducible approach. Co-precipitation method has been adopted for synthesis of nanoparticles of Fe3O4 and Co3O4. Scanning electron microscope (SEM), X-rays powder diffraction (XRD) and Raman spectroscopic characterization tools have been utilized to look through the morphology, compositional purity, crystallinity and emission characteristics of the fabricated magnetic nanoparticles. The study of magnetic measurement of Fe3O4 and Co3O4 nanoparticles was carried out in order to confirm their ferromagnetic behaviour, which could be attributed to the uncompensated surface spins and/or finite size effects. The magnetic study depicts that the ferromagnetic order of the Fe3O4 and Co3O4 nanoparticles is raised with increasing the decomposition temperature. Furthermore, in one set of experiment, the potentiometric urea biosensor was fabricated by drop casting the initially prepared isopropanol and chitosan solution, containing Fe3O4 nanoparticles, on the glass fiber filter (2cm diameter). To extract the voltage signal from the functionalized nanoparticles, a copper wire (thickness ~500 μm) has been utilized. The functionalization of surface of the Fe3O4 nanoparticles is obtained by the electrostatically immobilization of urease onto the nanobiocomposite of the Fe3O4-chitosan (CH). Urea biosensor with enhanced sensitivity, specificity, stability and reusability was fabricated. Electrochemical detection procedure has been adopted to measure the potentiometric response over the wide logarithmic concentration range of the 0.1 to 80 mM. Urea biosensor based on Fe3O4 nanoparticles depicts good sensitivity with 42 mV per decade at room temperature. In other set of experiment, a potentiometric urea biosensor has been fabricated through the immobilization of urease enzyme onto Co3O4-CH hybrid nanobiocomposite on glass filter paper and a copper wire (500μm diameter) has been attached with nanoparticles to extract the voltage output signal. The shape, size and dimensions of the Co3O4 magnetic nanoparticles were investigated by scanning electron microscopy (SEM), and diameter of nanoparticles lies in the range between Abstract 2 80-100 nm. The structural quality of the Co3O4 nanoparticles is confirmed from X-ray powder diffraction (XRD) measurements while the Raman spectroscopy has been used to understand the chemical bonding between the different atoms. A physical absorption method has been adopted to immobilize the enzyme on to the surface of Co3O4-CH hybrid nanobiocomposite. The potentiometric sensitivity curve measured over the large concentration range 0.1 - 80 mM of urea electrolyte and it revealed that the fabricated biosensor holds good linear sensing ability with a slope curve of the 45mV / decade. Besides magnetic nanoparticles, non magnetic nanoparticles silver (Ag) was also exploited for the fabrication of urea biosensor. Magnetic nanoparticles of Co3O4 showed better sensitivity response of 45mV per decade in comparison to that of Fe3O4 and Ag nanoparticles sensitivity response of 42 mV per decade. Presented biosensors depict good reusability, selectivity, reproducibility; resistance against interferers along with the nice stable output response of ~12 seconds. Moreover, proposed biosensor was used for determination of urea concentration in urine and blood samples of healthy and sick people. Comparing the results with laboratory data indicates that results were consistent with the laboratory data. Keywords: Cobalt oxide (Co3O4), iron oxide (Fe3O4), magnetic nanoparticles, potentiometry, urea biosensor, chitosan, magnetic studies.
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جد دا یار سیانا ہویا

جد دا یار سیانا ہویا
ساتھوں دور ٹھکانا ہویا

کول وی آکے ملدا نہیں
مٹی کھیہ یارانہ ہویا

کرسی اوہ مخلوق دی خدمت
جِنّے رب نوں پانا ہویا

پہلے تاں ہک پل نہ وسدا
ہن کیوں یار بیگانہ ہویا

رکھ اڈیکاں میں جا ستا
خواباں وچ یرانہ ہویا

مستی وچ کئی سجدے کیتے
جد دا میں فرزانہ ہویا

شمع نے ہک دم ساڑ جلایا
عاشق جد پروانہ ہویا

جس درود و سلام نہ بھیجے
عاشق کیویں یگانہ ہویا

مستی اپنی اینویں لگے
یار دا مکھ مستانہ ہویا

ذکر فکر وچ تیرے رہنا
ایہو ای تانا بانا ہویا

الصراع الجيوسياسي الإقليمي وعلاقته بموارد الطاقة في الدول المطلة على الخليج العربي

تهدف هذه الدراسة الى التعريف بمحاور الصراع الجيوسياسي الإقليمي المتنافسة من اجل السيطرة على موارد الطاقة بغية الوقوف على مخططاتها وأهدافها الجيوستراتيجية التي تسلكها لتحقيق أهدافها الانية وتطلعاتها المستقبلية، ومن اجل تحقيق تلك الأهداف المرجوة فقد تم الاعتماد على المنهج التحليلي الذي يعتمد عليه في الدراسات الجغرافية السياسية والجيوبولتيك، وقد توصلت الدراسة الى جملة من النتائج ابرزها ان موارد الطاقة التي تزخر بها منطقة الخليج العربي من النفط والغاز الطبيعي جعلت منها ارضية خصبة للصراعات الإقليمية والدولية نظرا لتحرك محاور هذه الصراعات بقوة من اجل ايجاد موطئ قدم لها في هذه المنطقة الحيوية والظفر بهذه الموارد الطاقوية واحكام السيطرة عليها وضمان استمرارية تدفها وابعاد القوى الفاعلة الاخرى من الوصول اليها

A Framework for Mining Emerging Trends in Web Clickstreams With Particle Swarm Optimization

The expansion of World Wide Web (WWW) in its size and exponential growth of its users has made the web most powerful and dynamic medium for information dissemination, storage, and retrieval. Moreover, the improvements in data storage technology have also made it possible to capture the huge amount of the user interactions (clickstreams) with the websites. The availability of such a huge amount of web user clickstreams has opened the new challenges for researchers to explore the weblog for the identification of hidden knowledge. For the last decade, web usage mining is playing a crucial role in the identification of trends from user clickstreams such as web personalization; user profiling; and user behavior analysis. These trends are beneficial in many ways such as information retrieval, website administration and improvement; customer relationship management; e-marketing; and recommender systems. The plenty of techniques are available in the literature, however, the accuracy, correctness and validity of the generated trends is totally relying on the proper selection of web mining process such as web sessionization, which is the benchmark for the later web usage mining stages. For the promising and optimized results, weblog sessionization is the eventual choice. Moreover, the extraction of proper, accurate and noise free sessionization is a demanding and challenging job in the presence of huge web clickstreams. The sessionization problem may fail to identify the focused and visualized groups from clickstreams records with high coverage and precision. Even though the well-known web session similarity measures such as Euclidean, Cosine, and Jaccard are prevalent in literature for mining process at the early learning stages. The web sessionization must take account of the validity of generated trends, which entirely depends upon the correctness and credibility of web sessions. To overcome the limitations of existing web sessionization techniques, we propose a Framework for Mining Trends (F MET) that empowers us to gauge the user activities on the website through evolutionary hierarchical Sessionization. Hierarchical Sessionization enhances the visualization of user click data to improve the business logic and mines the focused groups for scalable tracking of user activities. The foundation of the proposed framework is the swarm based optimized clustering technique along with a proposed web session similarity measure ST Index to address the Hierarchical Sessionization problem. The proposed web session similarity measure ST Index for hierarchical sessionization overcomes the limitations of Euclidean, Cosine and Jaccard measures, which may have failed to explicitly seek the proper and accurate trends. The Euclidean measures are of numerical in nature while the weblog data is of mixed nature. Moreover, existing measures are best for independent and isolated clustering groups. The proposed similarity measure ST Index computes the similarity among the user sessions through the common features (pages) shared among the sessions while assigning weight to uncommon features among the given sessions along with the minimum time shared by the given sessions time ratio. We validated and verify the proposed framework on three different datasets. The proposed ST Index measure produced the accurate and valid relationship among the sessions against common web session similarity measures. Furthermore, framework also produced the correct, accurate and valid trends. The performance of the proposed framework is validated against the well-known data analysis metrics such as VC (visitor coherence), accuracy, coverage and F1 Measures. The results show the significance improvements over the existing techniques of hierarchical Sessionization