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Synthesis and Characterization of Polymers/Ferrite Nano-Composites to Enhance Their Application in Biomedical Field

Thesis Info

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Author

Mushtaq, Muhammad Waheed

Program

PhD

Institute

University of the Punjab

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Chemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12627/1/Muhammad%20Waheed%20Mushtaq_Chem_2018_UoPunjab_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727462114

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Multidimensional magnetic cobalt ferrite (CoFe2O4) nanoparticles (NPs) were synthesized by a co-precipitation method under the effect of three capping agents called sorbitol, tergitol-1x (T-1x) and didecyldimethyl ammonium bromide (DDAB). The synthesized cobalt ferrite nanoparticles (CFNPs) were functionalized with guar gum (GG), xanthan gum (XG), gum arabic (GA), polymethacrylic acid (PMAA) and were subsequently characterised by Fourier transform-infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), X-ray diffraction (XRD) and contact angle studies. Vibrating sample magnetometry (VSM) was used for magnetic measurements of the native and polymers-coated CFNPs. The microstructural morphology of the uncoated and polymers-coated CFNPs was established using scanning electron microscopy (SEM), Transmission electron microscopy (TEM), atomic force microscopy (AFM) and dynamic light scattering (DLS) studies. The antibacterial activity of native and coated CFNPs was also tested against Escherichia coli and Staphylococcus aureus by using disc diffusion method. Furthermore, the interaction of cobalt ferrite nanoparticles with calf-thymus DNA was investigated and characterized by FT-IR. The biocompatibility of naked and coated CFNPs was also examined against normal (CHO) and cancerous (Huh-7) cell lines by performing MTT assay. Finally, the doxorubicin release profile from the drug-loaded functionalised CFNPs in the presence of an externally applied magnetic field was evaluated using UV-visible spectroscopy. The results show that the DDAB is more effective capping agent to control the particle size and dispersion of CFNPs in aqueous medium. Antibacterial activity test and MTT assay exhibited that coated-CFNPs are significantly more biocompatible as compared to native CFNPs. Polymer coating decreased the contact angle of the native CFNPs from 92° to 40°, which indicated that it modified the CFNPs surface from hydrophobic to hydrophilic. VSM analysis demonstrated that polymers coated cobalt ferrite NPs (CoFe2O4.NPs@polymers) also retained the magnetic characteristics of the bare cobalt ferrite NPs (Ms ∼77.2 emu/g), endorsing their application as promising magnetic nanovectors (MNV). The synthesised drug loaded-polymer coated cobalt ferrite NPs, (CoFe2O4.NPs@polymers-Doxo) exhibited significantly higher controlled discharge of doxorubicin at acidic pH (5.0) than at neutral pH (7.4). In vitro cytotoxic studies confirmed the cytocompatibility mode of coated CFNPs against Chinese Hamster Ovary and Huh-7 cell line, while 0.2 mg.mL1- dose of drug loaded magnetic nanocarriers inhibited the cell viability of Huh-7 up to 60%. These results strongly encourage the utilization of biocompatible magnetic nanocarriers (CoFe2O4.NPs@polymers) in targeted drug delivery territory for doxorubicin.
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راجے دے طوطے

راجے دے طوطے

پرانے زمانے دی گل اے کہ اک پنڈ وچ راجہ ناں دا اک بندہ رہندا سی۔ اوس نوں پالتو جانور تے پرندے پالن دا بہت شوق سی۔ اوس نے بکری، بھیڈ، مجھ، کتا، بلی تے خرگوش توں وکھ کئی پرندے وی پالے ہوئے سن۔ اوس نوں جتھوں وی کوئی سوہنا جانور یاں پرندا ملدا اوہ خرید لیندا تے فیر بہت محنت تے دل نال اوہناں نوں پالدا۔ پرندیاں وچ بھانویں کہ اوس کول تتر، کبوتر تے چڑیاں وڈی تعداد وچ سن پر اوس دا دل نئیں سی بھردا۔ اوہ پرندیاں دی تعداد وچ آئے دن کوئی نواں پرندیاں خرید کے وادھا کردا رہندا۔ اک دفعہ اوہ ایران دی سیر کرن گیا۔ اوتھے اوس نوں کجھ طوطے پسند آئے جو گلاں کردے سن۔ واپس تے اوس اوہ طوطے خریدے تے بڑی حفاظت نال اپنے گھر لے آیا۔ اوہ ایہناں دے بولن پاروں بہت خوش سی۔ اوہ سارا دن ایہناں نال گلاں کردا رہندا۔ جس پاروں طوطے ایس دے گھل مل گئے سن۔ طوطے بہت تیز ہوگئے سن۔ اوہ ہر روز راجے کولوں کدے چوری منگدے تے کدے کوئی پھل، کدے کھان نوں ہری مرچ یاں سبزی منگدے تے کدے بادام۔ راجہ اوہناں دی ہر خواہش پوری کردا سی۔

اک دفعہ نالدے پنڈ وچ میلہ لگا۔ اوس میلے وچ دوروں دوروں لوک اپنے پالتو جانور تے پرندے نمائش لئی لے کے آئے۔ اکثر لوک بیل، گاواں، مجھاں، کتے، بلیاں، خرگوش، گھوڑے، اونٹ لے کے آئے۔ کجھ لوک نچن والے گھوڑے لے کے آئے۔ ہن میلے دے میدان وچ واری واری لوک اپنے جانور یاں پرندے لے کے آندے تے اوہناں دے کرتب وکھاندے۔ سارے لوک اوہناں دے کرتب ویکھ کے بہت خوش ہوندے تے تاڑی وجا کے داد دیندے تے اپنی خوشی دا اظہار کردے۔

راجہ وی...

The Social and Financial Performance of Conventional and Islamic Microfinance Institutions in Pakistan

The financing operations of conventional microfinance institutions are usually based on interest (Usury/Riba) which is strictly prohibited by the Shariah of Islam, therefore, some Islamic microfinance institutions were set up in Pakistan to provide micro credit and other financial help to the deserving people based on Shariah compliant mechanism. The aim of this paper is to evaluate and compare the social and financial performance of these microfinance institutions in Pakistan. Two separate samples containing two microfinance institutions each, representing conventional and Islamic microfinance institutions has been selected for this study. Four stars, Wasil Foundation and Akhuwat from Islamic microfinance institutions while Asasah and Community Support Concern (CSC) from conventional microfinance institutions, rated by Mix market have been selected for this research paper. The social and financial performance based on outreach, profitability, efficiency/productivity, and portfolio quality of both these microfinance institutions were studied and compared. The study revealed that Islamic MFIs were more cost effective compared to conventional MFIs based on cost per borrower (CPB) and operating expenses to assets (OEA), while on the basis of financial efficiency conventional MFIs performed well. Though the financial and social performance of both Islamic and conventional MFIs have improved over the passage of time, still they have to struggle hard on various fronts especially to improve their profitability based on ROA and ROE measures to make the institutions profitable and sustainable. This study reveals that the successful operation of Akhuwat and Wasil Foundation for the last more than a decade latterly proves that Islamic MFIs are viable and sustainable even in the absence of charging interest from their clients. So, the society and the government should encourage and promote these Shariah compliant organizations in order to help the extremely marginalized people of the society.

Role of Plant Growth Promoting Rhizobacteria and Chemical Fertilizers on Plant Growth, Oil Content and Quality of Safflower and Canola and Their Potential for Biodiesel Production

Experiments were conducted to evaluate the role of plant growth promoting rhizobacteria (PGPR) alone and supplemented with chemical fertilizers to improve quantity and quality of safflower (Carthamus tinctorius L.) and canola (Brassica napus L.) with perspective to biodiesel production. First experiment of the series was conducted to evaluate the effect of PGPR viz. Azospirillum brasilense and Azotobacter vinelandii and chemical fertilizers (Urea and DAP) alone and in combination (under axenic conditions) on enzymes activities of rhizospheric soil in addition to general impact on growth of safflower cvv. Thori and Saif-32. The PGPR were applied as seed inoculation at the rate of 10 6 cells/mL prior to sowing. Chemical fertilizers were applied at full (Urea 60 Kg ha -1 and Diammonium phosphate (DAP) 30 Kg ha -1 ), half (Urea 30 Kg ha -1 and DAP 15 Kg ha -1 ) and quarter doses (Urea 15 Kg ha -1 and DAP 7.5 Kg ha -1 ) during sowing. The colony forming units (cfu) of Azospirillum and Azotobacter were higher in the presence of quarter dose of chemical fertilizers. Activities of soil enzymes viz. urease and phosphatase were enhanced by Azotobacter in combination with quarter doses of chemical fertilizers and Azospirillum in combination with half dose of chemical fertilizers respectively whereas; root proliferation was enhanced by Azotobacter and Azospirillum supplemented with half and quarter doses of chemical fertilizers. The 2 nd experiment was focused on the effect of PGPR and chemical fertilizers under field conditions on plant growth, seed yield, oil contents and quality of safflower with perspective to biodiesel production. The indole acetic acid, gibberellic acid, oil contents, oil/protein ratio and seed phenolics were improved by Azospirillum with half dose of chemical fertilizers. Azospirillum in combination with quarter dose of chemical fertilizers improved the fatty acid profile, oil quality and amino acids contents with parallel increase in biodiesel yield whereas; Azotobacter in combination with quarter dose of chemical fertilizers improved seed crude protein and induced protein of 130 KDa and 100 KDa. Seed nutrients viz. Ca +2 , K + were increased by Azotobacter and Azospirillum supplemented with half dose of chemical fertilizers. In the 3 rd experiment effects of chemical fertilizers, Azospirillum and Azotobacter were studied on plant growth, seed yield and oil quality of canola (Brassica napus L.) var. Pakola pertaining to biodiesel production. Significant improvements in seed yield ixand seed size were recorded in chemical fertilizer treatment while oleic acid (C18:1) was improved by Azospirillum treatment with parallel decrease in erucic acid contents. Azotobacter showed maximum increase in seed oil content with concomitant decrease in seed glucosinolate and moisture content. Chemical fertilizers and Azospirillum decreased the oil acid value and free fatty acid (%FFAs) contents with concomitant increase in seed protein and biodiesel yield (93 % and 92% respectively). Protocol for the production of safflower biodiesel was optimized using 1 H NMR, FT- IR, GC-MS and refractometer techniques. The biodiesel samples prepared by base catalyzed transesterification reaction showed maximum yield (97.84%) at 0.5% catalyst concentration with 6:1 methanol/oil ratio at 65°C as quantified by 1 H NMR technique with minimum (1.41) refractive index and improved physico-chemical properties. The GC/MS analysis showed the presence of five major faty acid methyl esters. Improvement in separation and quantification of fatty acid methyl esters in safflower oil was achieved using comprehensive two-dimensional GC (GC×GC). The GC×GC separation accomplished by the combination of SLB-IL111 with IL59 column phases provided excellent separation of FAME standard mixture and also safflower FAMEs were well separated and quantified in a short run of 16 min. It is inferred that PGPR can supplement the chemical fertilizers upto 50%–75% in order to obtain optimum growth and yield of safflower and use of advanced technologies improved biodiesel and fatty acid analyses being more rapid, precise and cost effective.