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Synthesis and Characterization of Potentially Biologically Active Phenoxy Iso-Butyric Acid Derivatives.

Thesis Info

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Author

Sheikh, Asma

Program

PhD

Institute

University of the Punjab

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Chemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/11833/1/Asma%20Sheikh%20chemistry%202019%20uop%20lhr%20prr.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727464240

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The work in this thesis describes the synthesis and characterization of phenoxy isobutyric acid derivatives and their biological screening which includes anti-bacterial, antioxidant, biofilm inhibition and mutagenic assay. First part of this research work consists of alkylation of substituted phenols to get 4-chlorophenoxy isobutyric acid (131) and 4bromophenoxy isobutyric acid (179). These acids were converted into 4-chlorophenoxy isobutyrate (132) and 4-bromophenoxy isobutyrate (180) followed by their hydrazinolysis to obtain 2-(4-chlorophenoxy)-2-methyl propane hydrazide (133) and 2-(4-bromophenoxy)-2methyl propane hydrazide (181).Condensation of these hydrazides 133 & 181.with substituted benzaldehyde and aromatic acetophenone yielded N’-benzylidine-2-(4-chlorophenoxy)-2ethylpropanehydrazides (155-178) and N’-benzylidine-2-(4-bromophenoxy)-2- methyl propane hydrazides (182-196) respectively. In another series, chalcones (110-130) were synthesized by Claisan condensation reaction. These chalcones were react with 2-(4-chlorophenoxy)-2-methylpropanehydrazide (133) in ethanol and acidic environment to yield a novel series of 2-(4-chlorophenoxy)-[1,diphenylallylidene]-2-methylpropanehydrazides. (134-154). In the second part, all the compounds were characterized by spectroscopic techniques like IR, NMR, mass spectrometry and X-ray crystallography. In third part, all the compounds were subjected to biological screening, which includes anti-bacterial, anti-oxidant, biofilm inhibition, and mutagenic assay and it was found that these compounds have a great potential to act actively in drug discovery.
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مستری چراغ دین

مستری چراغ دین (۱۹۳۵۔۱۸۵۷) پسرور میں پیدا ہوئے۔ مستری اردو اور فارسی زبان میں شاعری کرتے تھے۔ ۱۹۲۶ء میں آپ مکہ مکرمہ چلے گئے واپس آ کر ’’حج کا ساتھی‘‘ کے نام سے سفر نامہ مرتب کیا۔ خواجہ حسن نظامی نے اس سفر نامہ کو حلقہ مشائخ دہلی کے تحت مارچ ۱۹۲۸ء میں شائع کیا۔ بہت کوشش کے باوجود آپ کا شعری کلام دریافت نہیں ہو سکا۔ ایک شعر ملاحظہ ہو جو انہوں نے اپنے تعمیری مکان کے بیرونی دروازے کی ڈاٹ پر کنندہ کرایا تھا۔

مستری کو فکر تھا تاریخ کا                              کہ دیا ہاتف نے قصر دل پذیر      (۳۱)

۳۱۔         ڈاکٹر سلطان محمود حسین،’’تاریخِ پسرور‘‘لاہور‘ سنگ میل پبلی کیشنز‘ ۱۹۸۱ء ‘ ص : ۲۷۱

 

PEMBINAAN JAMAAH TABLIGH MELALUI PENGUATAN MODAL SOSIAL DI KOTA PALU

Fostering of Muslims in particular is a necessity. Muslims need guidance to stay on their nature, namely peace, calm, and peace (congratulations). Therefore, to maintain and preserve the atmosphere (peaceful, calm, serene), then one of the da'wah movements that has taken a role is the Tablighi Jamaat. Forms of coaching that have been done include syuro (deliberation), ta'awun (help-help). The coaching is intended to be social capital in developing Muslims. Social capital is intended as an adhesive tool for the community, because it often occurs in Islamic internal conflict communities that will affect external conflicts (among religious believers). The formation of Muslims by the followers of Tabligh has clearly brought developments to Muslims, especially in the city of Palu, this development was marked by an increase in the number of halaqah from 2 halaqah (2001) to 12 halaqah (2018), and the development has benefited the community based at the mosque.

Effect of Varying Dietary Protein Levels on Growth Performance, Proximate and Elemental Composition of Thaila Catla Catla under Polyculture System from Southern Punjab, Pakistan

Fish received increased attention as a potential source of animal protein and essential nutrients for human diets. Artificial food is required to maintain a high density of fish in farming conditions, than the natural fertility of the water. Fish reared in farming systems have different nutritional requirements than those in the wild. So, well-balanced feeds are necessary nutritionally for pond culture. Growth rate, efficiency of feed conversion and chemical composition of fish have a pronounced effect on the quantity and quality of food consumed. The growth and feed conversion ratio of a fish is remarkable tool to compute the acceptability of artificial feed. Thus, the aim of the present study was to investigate the growth performance, proximate and elemental composition of Catla catla reared under polyculture system by feeding artificial fish diets containing different levels of crude protein. For the present study, four different artificial fish diets with 15% CP (CP-15), 20% CP (CP-20), 25% CP (CP-25) and 30% CP (CP-30) were prepared by using locally available ingredients. Feeding trials were conducted in twelve earthen ponds at three different sites of southern Punjab, Pakistan (Figure I), during July to December, 2014. Catla catla were stocked at the rate of 3942 fish/ hectare (2000 fish/acre), having 30% of studying Major Carp, Catla catla, with Labeo rohita (60%) and Cirrhinus mrigala (10%) in the ponds. Four different artificial diets containing 15%, 20%, 25% and 30% of crude protein (CP-15, CP-20, CP-25 and CP-30, respectively) were offered at the rate of 4% of body weight of fish stock in four different treatments, T1, T2, T3 and T4, respectively. To adjust the feeding rate and to observe growth of fish, sampling was done at an interval of 30 days. The feeding trials lasted for 180 days. Various growth parameters such as percent weight gain, specific growth rate (SGR) and feed conversion ratio (FCR) were calculated by using standard formulae, at the end of feeding trial. Proximate composition of fish samples from each treatment was analyzed. Water content was determined by the oven drying method. Ash content was determined by using a muffle furnace. Fat content was measured by extraction in a mixture of chloroform and methanol. Protein content was estimated by difference from the mass of other main constituents. Elemental concentrations of Cd, Fe, K, Na, Pb and Zn in the fish samples of Catla catla were determined by using Flame Atomic absorption spectrophotometer. Differences among treatment means were determined by Duncan’s Multiple Range Test at a p<0.05 level of significance. Presently reported study suggested that among the different dietary protein levels (15%, 20%, 25% and 30% CP), tested for the study of growth performance of Catla catla under polyculture system with high stocking density (2000 fish/acre), 25% CP showed better FCR, survival and production. Hence, best protein level for optimum growth of Catla catla was observed to be 25% and it was not significantly different from that achieved by 30% (p<0.05) dietary protein level. Hence feed containing 25% CP was found to be satisfactory for fish growth instead of 30% CP. The present study also revealed feasibility of higher stocking density (upto 2000 fish/acre) especially in polyculture system. Estimated slope (b) values for LWRs in all four studied treatments and for overall data were found within the expected range of 2.5 - 3.5. Results for LWRs indicated negative allometric growth pattern in T1, positive allometric in T3 and T4, while isometric growth pattern in T2 for overall data. Mean percentages of moisture, ash, fat and protein contents in different treatments were ranged 74.82-77.05, 3.26-4.51, 5.10-5.18 and 13.52-15.94%, respectively. The dietary protein level 25% and 30% in fish feed would result in quality improvement of the chemical composition of fish. Proximate body composition of Catla catla of treatments having 25% CP and 30% CP were similar and found to be nonsignificantly higher than those fed with 15% and 20% CP. Hence feed containing 25% CP was found to be satisfactory for fish growth instead of 30% CP. Predictive regression equations in the present study demonstrated that body size significantly influenced the proximate body composition of Catla catla. Mean Fe, K, Na and Zn concentrations (μgg-1) in wet weight of Catla catla were found 58.58-95.05, 237.89-262.71, 379.44-396.78 and 14.47-16.55 μgg-1, reared under polyculture system by feeding various levels of fish feed in different treatments. Cadmium and lead were found below detectable limits in the body of Catla catla in all studied treatments. Analyzed metal concentrations were found lower than the permissible limits, proposed by different international organizations. For all studied treatments, the metal concentrations increased in the order: Cd=Pb<Zn<Fe<K<Na in the present work. Elevated trend was noticed among the essential metals and decreased trend among the nonessential metals were observed in the fish. Potassium concentrations in whole body of Catla catla presented differences (p < 0.05), however, iron, sodium and zinc concentrations showed no significance difference among studied treatments. Moreover, size of the fish found significantly correlated with Na and K, while remained constant with Fe and Zn concentration. The present work will help to improve fish production as well as promote aquaculture industry in the country. It will also be helpful in popularization of high stocking fish culture and use of artificial diets in public and private sector within the country. However, further studies on growth performance and chemical composition of fish by feeding various levels of dietary protein levels and inclusion of other fish species and hybrid fish in polyculture farming system may help in enhancing the production.