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Home > Synthesis, Characterization and Pharmacological Evaluation of Nano-Particles Prepared by Using the Extracts of Viola Pilosa and Shimmia Laureola

Synthesis, Characterization and Pharmacological Evaluation of Nano-Particles Prepared by Using the Extracts of Viola Pilosa and Shimmia Laureola

Thesis Info

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External Link

Author

Panni, Maria Khan

Program

PhD

Institute

The University of Agriculture

City

Peshawar

Province

KPK

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Biotechnology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/9933/1/Maria%20Khan%20Panni_Biotech_2018_UAP_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727517301

Similar


Multiple antibiotic resistant strains of microorganisms are imposing a dire necessity for novel and consistent drugs. Reliable natural products with negligible side effects are required to resist these pathogenic assailants. Investigation of medicinal plants for pharmacological and qualitative properties is substantial for appropriate choice and treatment of various diseases. The current study was aimed to Biosynthesize and characterize nanoparticles of boiled extract of plants and to evaluate different extracts of the plants qualitatively. The gold and silver nanoparticles (AuNPs and AgNPs) were successfully synthesized from solvent extracted methanolic crude and aqueous fractions (50mg plant extract / 100 ml deionized H2O) of the test plants under study. A solution of .1 mM of AuCl3 was shaken with Viola crude, aqueous and Skimmia stem aqueous extracts in 4:1 while 3:1, 2:1 and 1:1 in other samples for the production of gold (Ag) nanoparticles (NPs). For the synthesis of AgNPs from test plants, a solution of .1 mM of AgNO3 was combined with Viola shoot crude, aqueous and Skimmia leaves crude extracts in 10:1 (10 ml of AgNO3 and 1ml extract). Similarly, 1:5 (1ml of AgNO3 and 5ml extract) with Viola root crude, aqueous and Skimmia stem and leaves aqueous extracts and 1:10 stem crude extracts. Visual observation in color change from yellowish to dusky purple and dark brownish was taken as initial formation of silver and gold nanoparticles respectively. The formation of NPs synthesis was reaffirmed by the existence of observed peaks in the optimum range of 350-450 nm (silver Nps) and 500-600 nm (gold Nps) through UV-Vis spectrophotometer. The gold and silver nanoparticles of Viola were found highly stable at the temperature range between 25°C and 50°C and that of Skimmia between 20°C and 40°C and more stable at salt stress in milli-Molar concentrations as compared to molar ones. The X-Ray diffraction showed crystalline, spherical structure, and centro symmetric and cubic nature of gold and silver nanoparticles. The results of SEM confirmed the size of AuNPs between the range of 20 nm – 50 nm and that of AgNPs in 20 nm – 80 nm range. The FT-IR studies showed that phenols and Esters were accountable for the green production of the Gold and Silver nanoparticles (AuNPs and AgNPs). The silver nanoparticles (AgNPs) were found more active in regulating the development of bacterial and fungal strains as compared to gold nanoparticles (AuNPs). More significant results were recorded for roots of Viola pilosa (90.25%) and leaves of Skimmia laureola (86.79%) against bacterial strains. Similarly, shoots extracts of Viola pilosa (95.02%) and stem extracts of Skimmia laureola (97.29%) were found more efficient in antifungal activities. The antioxidant potential of nanoparticles proved that they were active in scavenging free radicals possessing maximum antioxidant potential of 80.86% when compared to the xix control. Similarly, roots extracts of Viola pilosa were by far most potent in scavenging free radicals amongst all the other solvent extracts and showed much significant antioxidant activities of 95.87% at 250 μg/ml. The plants also presented significant level of phytotoxic (97.76%) and insecticidal activities (30%). Phytochemical study of the test plants discovered that they were abundant in Tannins, Carbohydrates, Sterols, Proteins and Lipids. Based on these results, both the tested plants are highly recommended for Green synthesis of nanoparticles as well as efficient Antibacterial, Antifungal and Antioxidant agents.
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ہم بھی ہوتے ہیں کہ عشاق جہاں ہوتے ہیں

ہم بھی ہوتے ہیں کہ عشاق جہاں ہوتے ہیں
ہم سے آزاد منش اور کہاں ہوتے ہیں

تم نے جانا ہے تو پلکوں پہ ستارے کیوں ہیں
فیصلے ضبط کے یوں سب پہ عیاں ہوتے ہیں

جب تلک سانس ہے اک آس لگی ہے ہمدم
سانس کی ڈور جو ٹوٹے تو زیاں ہوتے ہیں

میں مقید ہوں تری چشم کے ایوانوں میں
حوصلے اڑنے کے بھی مجھ سے کہاں ہوتے ہیں

تم ہو دھڑکن مرے سینے میں مچلتے دل کی
خون میں ایسے جنوں زاد رواں ہوتے ہیں

مال و دولت سے فضاؔ ان کو غرض کچھ بھی نہیں
عشق والوں کے الگ سود و زیاں ہوتے ہیں

الدعوة الاسلامية دعوة عالمية

Allah almighty has created Human beings, the angels, and jinn for His worship alone. To guide the human beings to the right path, Allah almighty has sent many messengers to all nations and pieces of lands. Hazrat Adam (A. S.) was the first human being as well as the first Prophet of Allah on the Earth, while the Prophet Muhammad (PBUH) was the last of all in this chain. There will be no prophet after him. Now, it is the duty of every Muslims to carry and spread this universal message to the whole humanity. In this context, the message of Islam is universal. This article deals with this issue through giving proofs from the Holy Qur'an and Sunnah.

Precision Irrigation for Improving Water Use Efficiency Using Indigenized Sensor Based Irriagion Scheduling and Efficient Irriagion Techniques

Real time sensors based precision irrigation scheduling has potential to improve water productivity. Field experiments were carried out for two subsequent years (2017 and 2018) for producing maize and wheat crops at the Water Management Research Center (WMRC), Postgraduate Agricultural Research Station (PARS), University of Agriculture, Faisalabad (UAF). Field irrigation systems included flood irrigation (canvas pipe), perforated pipe irrigation and drip irrigation under different planting geometries. The design, development and manufacturing of sensor-based irrigation systems using locally available material were performed to minimize the cost of equipment development and energy consumption for crop irrigation. A solar powered tube well of half cusec discharge was used for pumping water for irrigating the experimental fields. The fertigation unit was used with electricity operated submersible pump at the experimental site. Maize crop had seven treatments viz; T1-flood irrigation conventional ridge sowing (0.762 m row to row spacing), T2-flood irrigation furrow bed planting (0.457 m row to row spacing), T3perforated pipe irrigation with 0.254 m furrow width (0.432 m row to row spacing), T4- perforated pipe irrigation with 0.203 m furrow width (0.406 m row to row spacing), T5perforated pipe irrigation with 0.152 m furrow width (0.381 m row to row spacing), T6drip irrigation with 0.914 m lateral spacing (0.457 m row to row spacing) and T7-drip irrigation with 1.219 m lateral spacing (0.609 m row to row spacing). Similarly, wheat crop had also seven treatments viz; T1-flood irrigation flat sowing by rabi-drill, T2-flood irrigation bed furrow planting with 0.254 m furrow, T3-perforated pipe irrigation bed furrow planting with 0.254 m furrow, T4-perforated pipe irrigation bed furrow planting with 0.203 m furrow, T5-perforated pipe irrigation bed furrow planting with 0.152 m furrow, T6-drip irrigation flat with 0.914 m lateral spacing and T7-drip irrigation on beds with 0.914 m lateral spacing. The flood irrigation system (treatments T1 and T2) took more time to fill the field and resulted in significantly lowest water productivities of maize 9.2-9.72 (grain yield kg/ha/mm irrigation depth) and of wheat 9.6-10.30 (grain yield kg/ha/mm irrigation depth). The perforated irrigation system (treatments T3, T4 and T5) produced intermediate values of water productivities for maize 16.38-17.3 (grain yield kg/ha/mm irrigation depth) and for wheat 12.30-12.66. The drip irrigation system (treatments T6 and T7) resulted in significantly greatest water productivity values of 19.219.55 for maize crop and 14.20-14.30 for wheat crop. xlii Indigenized soil moisture sensors using copper, brass and steel materials of single probe (Type-I length: 152.4 mm, 304.8 mm), two probes (Type-II length: 152.4 mm, 304.8 mm) and Type-III length (152.4 mm, 304.8 mm) were developed, fabricated, calibrated and validated using Gravimetric Method and tested in field. The developed sensors sent soil moisture signals on cloud for data storage, reuse and sharing purpose using coding. Arduino Mega was coupled with laptop through USB cable and sensors responses shown on Arduino sketch 1.8.5 in serial monitor. Arduino Mega was coupled with Arduino Ethernet Shield for transformation of soil moisture data on cloud. The irrigation was applied based on soil moisture status. The system based on micro-controller was tested for irrigating Maize and Wheat crops. Raspberry Pi-3 (Model B) controlled hardware in distribution box (DB) made promising use of indigenized soil moisture sensors (Type-I, Type-II and Type-III) for calibration and irrigation water management. The Linear calibration for indigenized steel wired double probe soil moisture sensors (152.4 mm, 304.8 mm) was made. The costs incurred for developing Type-I, Type-II and Type-III soil moisture sensors were PKR 800, 1650 and 250, respectively. The WinSRFR model was used to determine water application efficiency as a function of each plot‟s irrigations with respect to experimental field length under sandy loam soil. For the crops grown during 1st year (2016-17), the model resulted in application efficiencies of maize as 44%, 41%, 63%, 67%, and 69% for treatments T1, T2, T3, T4 and T5, respectively. Similarly the model predicted application efficiencies of wheat as 55%, 64%, 61%, 66% and 75% under treatments T1, T2, T3, T4 and T5, respectively. These application efficiency improved in the 2nd year crops under the indigenized soil moisture sensors based precision irrigation scheduling. The drip irrigation treatments (T6 and T7) had significantly improved water application time saving values (65.26% and 61.38%), perforated pipe irrigation treatments (T3, T4 and T5) had intermediate values of water application time saving (15.52%, 12.76% and 10.07%) as compared to T1-conventional ridge sowing (canvas pipe/flood irrigation). Similarly, drip irrigation treatments (T6, T7) had significantly better water application time saving values (66.12%, 62.33%), perforated pipe irrigation treatments (T3, T4 and T5) had intermediate values of water application time saving (17.60%, 14.91% and 12.29%) as compared to T2-furrow bed planting (canvas pipe/flood irrigation). Application time saving was 58.88%, 60.18% and 61.37% using drip irrigation treatment (T6) as compared to perforated pipe irrigation treatments (T3, T4 and T5). Similarly, water xliii application time saving under drip irrigation treatment (T7) was 54.28%, 55.73% and 57.05% as compared to perforated pipe irrigation treatments (T3, T4 and T5). All the treatments under perforated and drip irrigation systems had better water application time saving values during 2nd year maize cropping than those under 1st year maize cropping. The drip irrigation treatments (T6, T7) for wheat sowing had significantly better water application time saving values (44.82%, 37.42%), perforated pipe irrigation treatments (T3, T4 and T5) had intermediate values of water application time saving (27.73%, 23.33% and 17.72%) as compared to T1-flat sowing by rabi drill (canvas pipe/flood irrigation). Similarly, drip irrigation treatments (T6, T7) had significantly highest water application time saving values (40.81%, 32.87%), perforated treatments (T3, T4 and T5) had intermediate values of water application time saving (22.47%, 17.75% and 11.74%) as compared to T2-bed furrow planting (canvas pipe/flood irrigation). Application time saving was 23.65%, 28.03% and 32.94% using drip irrigation treatment (T6) as compared to perforated pipe irrigation treatments (T3, T4 and T5). Similarly, water application time saving was 13.42%, 18.38% and 23.95% using drip irrigation treatment (T7) as compared to perforated treatments (T3, T4 and T5). All the treatments under perforated and drip irrigation systems had higher water application time saving values during 2nd year wheat cropping than those under 1st year of wheat cropping. Efficient water application in the experimental field for maize and wheat crops increased irrigation efficiency. For maize production, drip irrigation treatments (T6, T7) had significantly improved irrigation efficiency values (86.0%, 86.83%), perforated treatments (T3, T4 and T5) had intermediate values (80.83%, 81.0% and 81.05%) and flood irrigation treatments had significantly lowest values (50.95%, 52.15%). All the treatments had significantly greater irrigation efficiency values during 2nd year maize cropping than those under 1st year maize cropping because of applying soil moisture sensor based irrigations. The drip irrigation treatments (T6, T7) had significantly greatest irrigation efficiency values (87.95%, 88.1%), perforated treatments (T3, T4 and T5) had intermediated values of irrigation efficiency (80.5%, 80.75% and 81.45%) and flood irrigation treatments had significantly lowest irrigation efficiency values (73.25%, 74.4%). Most of the treatments had significantly higher irrigation efficiency values during 2nd year wheat cropping than xliv those under 1st year wheat cropping. Overall treatment mean irrigation efficiency was 80.82% during 1st year and 81.0% during 2nd year. Total cost of production of 1st year maize (2016) for the drip irrigation treatments T6 and T7 was found higher than the perforated pipe irrigation system and flood irrigation system with a margin of PKR 68451 and PKR 68292, respectively. The drip irrigation produced a benefit cost ratio of 3.28 for T6 and 3.20 for T7 treatments. The benefit cost ratio of perforated pipe treatments T3, T4 and T5 were 3.43, 3.41 and 3.26, respectively. Similarly, the benefit cost ratio for flood irrigation treatments T1 and T2 were 2.68 and 2.77, respectively. Total cost of production of 2nd year maize (2017) for the drip irrigation treatments T6 and T7 was found higher than the perforated pipe irrigation system and flood irrigation system with a margin of PKR 74443 and PKR 74281, respectively. The drip irrigation produced a benefit cost ratio of 3.14 for T6 and 3.06 for T7 treatments. The benefit cost ratio for perforated pipe irrigation was 3.33, 3.25 and 3.11 for T3, T4 and T5 treatments, respectively. Similarly, the benefit cost ratio for flood irrigation was 2.58 and 2.68 for T1 and T2, respectively. Total cost of production of 1st year wheat (2016-17) for the drip irrigation treatments T6 and T7 was found higher than the perforated pipe irrigation system and flood irrigation system with a margin of PKR 70726 and PKR 71214, respectively. The drip irrigation produced a benefit cost ratio of 2.81 for T6 and 2.77 for T7 treatments. The benefit cost ratio for perforated pipe was 2.94, 2.87 and 2.81 for T3, T4 and T5 treatments, respectively. Similarly the benefit cost ratio for flood irrigation was 2.51 and 2.61 for T1 and T2 treatments, respectively. Total cost of production of 2nd year wheat (2017-18) for the drip irrigation treatments T6 and T7 was higher than the perforated pipe irrigation system and flood irrigation system with a margin of PKR 64279 and PKR 64708, respectively. The drip irrigation produced a benefit cost ratio of 3.26 for T6 and 3.21 for T7 treatments. The benefit cost ratio for perforated pipe was 3.25, 3.18 and 3.12 for T3, T4 and for T5 treatments, respectively. Similarly, the benefit cost ratio for flood irrigation was 2.81 and 2.92 for T1 and T2 treatments, respectively. The benefit-cost analysis for drip and perforated pipe irrigation systems showed that the perforated pipe irrigation could be a feasible irrigation method for small scale farmers and drip irrigation system for large farmers. However, keeping in view the benefits of drip irrigation regarding high water use efficiency and yield, it is recommended that it should also be promoted for small farmers by providing proper training for profitable farming." xml:lang="en_US