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Home > Synthesis, Characterization and Pharmacological Evaluation of Nano-Particles Prepared by Using the Extracts of Viola Pilosa and Shimmia Laureola

Synthesis, Characterization and Pharmacological Evaluation of Nano-Particles Prepared by Using the Extracts of Viola Pilosa and Shimmia Laureola

Thesis Info

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External Link

Author

Panni, Maria Khan

Program

PhD

Institute

The University of Agriculture

City

Peshawar

Province

KPK

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Biotechnology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/9933/1/Maria%20Khan%20Panni_Biotech_2018_UAP_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727517301

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Multiple antibiotic resistant strains of microorganisms are imposing a dire necessity for novel and consistent drugs. Reliable natural products with negligible side effects are required to resist these pathogenic assailants. Investigation of medicinal plants for pharmacological and qualitative properties is substantial for appropriate choice and treatment of various diseases. The current study was aimed to Biosynthesize and characterize nanoparticles of boiled extract of plants and to evaluate different extracts of the plants qualitatively. The gold and silver nanoparticles (AuNPs and AgNPs) were successfully synthesized from solvent extracted methanolic crude and aqueous fractions (50mg plant extract / 100 ml deionized H2O) of the test plants under study. A solution of .1 mM of AuCl3 was shaken with Viola crude, aqueous and Skimmia stem aqueous extracts in 4:1 while 3:1, 2:1 and 1:1 in other samples for the production of gold (Ag) nanoparticles (NPs). For the synthesis of AgNPs from test plants, a solution of .1 mM of AgNO3 was combined with Viola shoot crude, aqueous and Skimmia leaves crude extracts in 10:1 (10 ml of AgNO3 and 1ml extract). Similarly, 1:5 (1ml of AgNO3 and 5ml extract) with Viola root crude, aqueous and Skimmia stem and leaves aqueous extracts and 1:10 stem crude extracts. Visual observation in color change from yellowish to dusky purple and dark brownish was taken as initial formation of silver and gold nanoparticles respectively. The formation of NPs synthesis was reaffirmed by the existence of observed peaks in the optimum range of 350-450 nm (silver Nps) and 500-600 nm (gold Nps) through UV-Vis spectrophotometer. The gold and silver nanoparticles of Viola were found highly stable at the temperature range between 25°C and 50°C and that of Skimmia between 20°C and 40°C and more stable at salt stress in milli-Molar concentrations as compared to molar ones. The X-Ray diffraction showed crystalline, spherical structure, and centro symmetric and cubic nature of gold and silver nanoparticles. The results of SEM confirmed the size of AuNPs between the range of 20 nm – 50 nm and that of AgNPs in 20 nm – 80 nm range. The FT-IR studies showed that phenols and Esters were accountable for the green production of the Gold and Silver nanoparticles (AuNPs and AgNPs). The silver nanoparticles (AgNPs) were found more active in regulating the development of bacterial and fungal strains as compared to gold nanoparticles (AuNPs). More significant results were recorded for roots of Viola pilosa (90.25%) and leaves of Skimmia laureola (86.79%) against bacterial strains. Similarly, shoots extracts of Viola pilosa (95.02%) and stem extracts of Skimmia laureola (97.29%) were found more efficient in antifungal activities. The antioxidant potential of nanoparticles proved that they were active in scavenging free radicals possessing maximum antioxidant potential of 80.86% when compared to the xix control. Similarly, roots extracts of Viola pilosa were by far most potent in scavenging free radicals amongst all the other solvent extracts and showed much significant antioxidant activities of 95.87% at 250 μg/ml. The plants also presented significant level of phytotoxic (97.76%) and insecticidal activities (30%). Phytochemical study of the test plants discovered that they were abundant in Tannins, Carbohydrates, Sterols, Proteins and Lipids. Based on these results, both the tested plants are highly recommended for Green synthesis of nanoparticles as well as efficient Antibacterial, Antifungal and Antioxidant agents.
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سید صباح الدین عبدالرحمن

آہ! سید صباح الدین عبدالرحمن!!
مسند شبلی و سلیمان اجڑ گئی
جناب سید صباح الدین عبدالرحمن ناظم دارالمصنفین و اڈیٹر معارف ہم سب کو چھوڑ کر اب خدائے رحمان کے آغوش رحمت میں چلے گئے، گذشتہ ماہ ۱۸؍ نومبر کو ٹیلیفون اور ریڈیو سے دن میں دو ڈھائی بجے کے درمیان ان کی المناک شہادت کی خبر دارالمصنفین میں بجلی بن کر گری اور ان کے متعلقین و وابستگان دارالمصنفین کی امیدوں اور آرزوؤں کے خرمن کو خاک و سیاہ کرگئی، مولانا سید سلیمان ندویؒ، مولانا عبدالسلام ندویؒ اور مولانا شاہ معین الدین احمد ندویؒ کے بعد دارالمصنفین کی بزم علم و ادب اسی چراغ سے روشن تھی لیکن افسوس کہ باد حوادث کے جھونکوں نے اسے بھی گل کردیا:
صبح تک تو نے نہ چھوڑی وہ بھی اے باد صبا
یادگار گل شمع تھی کل تک جو پروانے کی خاک
وہ بالکل ٹھیک تھے، ۱۰؍ نومبر کو لکھنؤ روانہ ہونے سے پہلے فجر بعد ہی مجھے نومبر کے معارف کے شذرات دیے اور ۹ بجے دن میں انھیں کاتب کے حوالے کرنے کی تاکید کی اور عین روانگی کے وقت فرمایا کہ ۱۹؍ نومبر کے بعد واپس ہوں گا، میں ان کی مشایعت کے لیے ان کے پیچھے ہوگیا تو مڑکر میری جانب دیکھا اور فرمایا کہ بیٹھیے! میں نے مصافحہ کے لیے ہاتھ بڑھایا تو انھوں نے مصافحہ کرتے ہوئے خدا حافظ کہا اور آگے بڑھ گئے، میری طبیعت نہ مانی اور میں ان کو رخصت کرنے کے لیے پھاٹک تک گیا، وہاں دوسرے رفقاء اور وابستگان دارالمصنفین بھی ان کو رخصت کرنے کے لیے پہلے سے موجود تھے، سب سے رخصت ہوکر وہ رکشہ پر بیٹھ کر روانہ ہوگئے، ۲۰؍ نومبر کو جمعہ تھا، جمعہ چھوٹ جانے کے اندیشہ سے وہ اس دن سفر نہیں کرتے، اس لیے خیال تھا کہ ۲۱؍ نومبر...

توریہ کی حقیقت، ضرورت اور اس کا حکم: ایک تحقیقی جائزہ

Islam is a religion of peace, love and prosperity. It emphasis on moral values and strongly forbids from immoral acts. Commitment to the former is ultimate way to the paradise, while the latter leads to Hell, which is an abode, where deviants from the righteousness are punished for their misdeeds. The Holy Prophet Muhammad (ﷺ) showed the importance of morality through the deeds and actions. “Ta┴riyā” is an act which is a part of morality. It is an act which escapes a human being from telling a lies strongly forbidden in Islam, being a root cause of manly social avoid. Usually“Ta┴riyā” is adopted so as to avoid differences and turmoil on a group of individuals. By“Ta┴riyā” means speaking a statement which is equivoques i.e. At the same it gives too meaning apparent and the hidden. Now the question arises whether “Ta┴riyā” is considered as lie or truth. It cannot be termed either lie or truth. However, in many traditions, it has been negated as a lie. At some occasions, when a person feels to tell a lie, for the sake of bringing peace, then it is advised than he should bring on use the act of “Ta┴riyā” but. The reality manipulated be in such a way so it could be termed as lie.

Genetic Analysis of Autosomal Recessive Intellectual Disability in Consanguineous Families

Intellectual disability (ID) is a common and highly heterogeneous neurodevelopmental disorder. It affects 1–3% of the world’s population and its prevalence is almost twice as high in underdeveloped than in affluent world. ID has an enormous socio-economic burden and devastating impact on the lives of affected individuals and their families. More than 700 genes have been discovered so far across different studies pertaining to X-linked, autosomal dominant and recessive ID. Autosomal recessive intellectual disability (ARID), being the most common form of ID. Till date, more than 577 genes involved in ARID have been discovered mostly in consanguineous communities. ARID is further grouped into syndromic ARID (nARID) and non-syndromic ARID (nsARID) types primarily based upon the phenotypic appearance. In Pakistan, the rate of consanguinity is approaching 70% and this high rate results in higher rates of recessive disorders including ARID. Inbred families provide a unique opportunity to find pathogenic variants in known as well as candidate genes responsible for recessive disorders due to the extensive regions of homozygosity in the genomes of these individuals. We enrolled 25 suspected consanguineous ARID families based on pedigree analysis and clinical evaluation from Kohat district, KPK province of Pakistan. The identified consanguineous families were subjected to genetic analysis through Whole exome sequencing (WES) and genome-wide SNP array. Bioinformatics analysis was carried out using Genome Analysis Tool Kit (GATK) based bioinformatics pipeline and Consanguinity Analysis Through Common Homozygosity (CATCH) software. Subsequent prioritization was carried out by application of various quality filters. Finally, the selected putative causative variants were validated by Sanger sequencing. Out of 25 families, known causative mutations in five known genes (ASPM, ATRX, GPR56, NAGLU and DOLK) were identified in eight different families for ARID. Furthermore, novel mutations in seven known ID genes (ABAT, SLC12A6, SHANK3, BCKDK, DDHD2, ERCC2 and GPT2) were found in seven families. Three potential likely pathogenic mutations were identified in three different families in three novel genes (DYNC1I2, FBXL3 and LINGO1). However, seven families remained unsolved; probably we missed the causative variants. Our study showed that v exome sequencing in combination with microarray genotyping is a powerful technique to find out the causative variants in rare ARID families and can be used to establish correlation between candidate genes and phenotypes. The diagnostic yield of the strategy used in our whole ID project was found to be approximately 32% (32/100). Furthermore, the present work also revealed that the genetic variations are associated with a significant number of consanguineous ARID families from Pakistan.