Search or add a thesis

Advanced Search (Beta)
Home > Synthesis, Spectroscopic Analyses and Biological Evaluation of Silver and Gold Based Pro-Nanomedicines Derived from Fluoroquinolones

Synthesis, Spectroscopic Analyses and Biological Evaluation of Silver and Gold Based Pro-Nanomedicines Derived from Fluoroquinolones

Thesis Info

Access Option

External Link

Author

Khan, Shujaat Ali

Program

PhD

Institute

University of Peshawar

City

Peshawar

Province

KPK

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Chemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/13044/1/Shujaat%20Ali%20Khan%20PDF.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727526327

Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.

Similar


The work presented in this dissertation comprises of synthesis, spectroscopic analyses and biological evaluation of silver and gold based pronanomedicines derived from fluoroquinolones. Among others, it includes convenient and time saving production of noble metals (Ag/Au) nanoparticles (NPs) capped with fluoroquinolone antibiotics (moxifloxacin (Mox), ciprofloxacin (Cip), sparfloxacin (Sp) and gemifloxacin (Gm). Different reducing agents such as triethylamine, hydroquinone and sodium borohydride were employed to transform Ag/Au salts into feasible capping agents. Among them, sodium borohydride relatively gave better results. As for we understand and based on FTIR data, the NH moiety of fluoroquinolones were mainly responsible for the capping of Ag/Au nanoparticles. In order to manifest alternate green method, Ag and Au NPs were also produced by using selected medicinal plants; Rhododendron arboreum (RA), Kigelia pinnata (KP) and Eulophia dabia (ED) as reducing and stabilizing agent, while triethylamine was used to synthesize NPs of the extract of Desmodium elegans (DE). The structural framework and size morphology of synthesized NPs were characterized by using advanced analytical techniques such as atomic force microscope (AFM), UV visible, fourier transform infrared spectroscope (FTIR), energy dispersive X-ray (EDX) and scanning electron microscope (SEM). To find alternate to wide spread resistive strains of pathogenic microbes; new antimicrobial agents are needed to treat the patients infected with such resistive pathogenic microbes. The locally synthesized pronanomedicines derived of fluoroquinolones were evaluated for biological properties namely urease inhibition, leishmanicidal, antimicrobial and antioxidant activities. Interestingly and as for our expectations, these NPs enhanced biological and pharmacological activities. The synthesized pronanomedicines and the capping ligands were independently screened for jack bean urease enzyme inhibition potential. Mostly, the Ag-Mox NPs exhibited higher level of enzyme inhibition activity of 93% at 0.2 mg/mL and IC50 value of 0.66 ± 0.042 μg/mL concentration, while the ligand; Mox revealed weak inhibition with IC50 value of 183.25 ± 2.06 μg/mL. On the other hand, the Au-Mox NPs remained inactive as compared to the parent ligand (Mox) having IC50 = 183.25 ± 2.06 μg/mL. These results reflect that after conjugation of Mox with Ag, the activity of moxifloxacin was significantly increased about 250 times. However, the urease inhibition activity of the Au conjugated counterpart of moxifloxacin decreased significantly. The synthesized metallic nano-conjugates (Ag-Cip and Au-Cip NPs) and the parent ligand, ciprofloxacin were also tested for jack bean urease enzyme inhibition potential. Ag-Cip pronanomedicine exhibited better urease enzyme inhibition indicating 96 % at 0.2 mg/ mL (IC50 = 1.181 ± 0.02 μg/mL) concentration. On the other hand, Au-Cip NPs showed comparatively weaker urease inhibition (90 % at 0.2 mg/mL concentration) with IC50 = 52.55±2.3 μg/mL. As anticipated, the parent ligand ciprofloxacin revealed weaker inhibition to the values of 75 % at 0.2 mg/mL and IC50 = 82.95 ±1.62 μg/mL concentrations. Furthermore, leishmanicidal, antimicrobial and antioxidant activities were tested for both synthesized pronanomedicines and all the parent ligands under discussion but they revealed good to moderate activities. The selected plants namely R. arboreum, K. pinnata, E. dabia and D. elegans and their metallic NPs were also screened for jack beans urease enzyme, leishmanicidal, antimicrobial and antioxidant activities which exhibited promising activities, while D. elegans-capped NPs showed moderate activities. Convincingly, the synthesized pronanomedicines were monodispersed and revealed stability to some extent by changing pH, concentration of table salt and temperature. The silver based pronanomedicines were anticipated to be good candidate for urease inhibition and leishmanicidal potentials.
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

مولانا مفتی محمد عتیق فرنگی محلی

مولانا مفتی محمد عتیق فرنگی محلی
مولانا مفتی محمد عتیق کا سانحہ وفات بھی مذہبی اور علمی حلقوں کے لئے باعث رنج و افسوس ہے، ہر چند کہ وہ عمر طبعی کو پہنچ چکے تھے، اور عرصہ سے بیمار رہتے تھے لیکن اس قحط الرجال کے زمانہ میں ان کا دم بساغنیمت تھا، وہ فرنگی محل کے اس خانوادہ سے تعلق رکھتے تھے، جس نے کئی سو برس سے تعلیم و تدریس، تصنیف و تالیف اور ارشاد و ہدایت کی شمع روشن رکھی ہے، اس خاندان کے فیوض و برکات سے ہندوستان ہی نہیں بلکہ دوسرے ممالک بھی مستفید ہوتے رہے ہیں۔
ملا نظام الدین کا مجوزہ نصاب تعلیم ایسا مقبول ہوا کہ سینکڑوں برس سے عربی مدارس میں رائج ہے، اور درس نظامی کی تکمیل دستار فضیلت کے حصول کے لئے ضروری سمجھی جاتی ہے، اس دو ڈھائی سو برس میں ساری دنیا میں انقلاب برپا ہوگیا، اور تعلیمی نصاب میں غیرمعمولی ردوبدل ہوا، لیکن ہندوستان میں عربی مدارس کی بڑی تعداد اب بھی درس نظامی پر جمی ہوئی ہے، یہ ملا نظام الدین اور ان کے جانشینوں کے خلوص اور کمال علم و عمل کا اثر ہے کہ عوام و خواص سب اس خاندان کی حلقہ بگوشی کو باعث فخر سمجھتے رہے، مل بحرالعلوم نے جب جنوبی ہند کا رخ کیا تو نواب ارکاٹ استقبال کے لئے آگے بڑھے اور ان کی پالکی کو کاندھا دیا۔
مولانا محمد عتیق صاحب اسی خاندان عالی کے ایک ممتاز فرد تھے، اس خاندان میں علم و عمل کا ایسا اجتماع رہا کہ فرنگی محل دارالعلوم کہلایا، ملا نظام الدین علمی کمال کے ساتھ شاہ عبدالرزاق ہانسوی سے ارادت رکھتے تھے، اس طرح اس خاندان میں علم و معرفت، معقول و منقول اور فقرو درویشی کا ہمیشہ اجتماع رہا۔ مغربی علوم و فنون کی چمک دمک سے سبھی...

آلودہ پانی کی مروجہ تحلیل و تطہیر کا سائنسی و شرعی جائزہ

Water symbolizes life on the earth. All kinds of existence depend on water. Allah has created great reservoirs of water on this earth which cater to the needs of all living beings, but human negligence is making these resources polluted. Pollution not only poses threats to human life, it also endangers marine life and resources. These threats have been increased manifold by industrial revolution and increasing human population. The same factors are also responsible for the increased use of water. Many countries have employed distillation processes to provide clean water for the increasing demands of population, agriculture and industries. Islamic jurisprudence also takes into account different types of water, some of which can be used in cleansing rituals for worship, while some cannot be used. The question arises whether the purified water by scientific process will be "Ṭahir & Muṭṭahir" and can be used for ablution and other Islamic rituals? Will Islamic Jurist accept it as "Istiḥalah"? The following study will outline the details of distilled water to see whether it can be used for daily chores and ablution rituals or not.

Evaluation of Organochlorine and Polychlorinated Biphenyl Residues in the Blood / Serum Samples of Cancer Patients With Reference to the Assessment of Health Risk of Karachi People Due to Environmental Pollutants

The present study was conducted for the comparative evaluation of Polychlorinated Biphenyls (PCBs) and Organochlorine Pesticides (OCPs) in the serum samples of diagnosed cancer patients and healthy humans of Karachi. Impact of these chemicals on the serum cell free DNA (cf-DNA), Cholesterol, Alkaline Phosphatase and Total Protein contents was also ascertained in the studied cohort. This was a preliminary work on the role of PCBs and OCPs in the etiology of cancer in Pakistan. A random collection of fasting blood samples from diagnosed cancer patients having various malignancies and healthy humans was carried out with informed consent of the donors at various hospitals and health care centers of Karachi. Serum was separated within 2 hours of collection and was used for subsequent analysis. Analysis of PCBs and OCPs was carried out on Gas Chromatograph coupled with Electron Capture Detector. Serum cf-DNA was analyzed on NanoDrop Spectrophotometer while serum Cholesterol, ALP and Total Protein contents were analyzed on Roche/Hitachi diagnostic analyzers. Levels of the seven tested PCBs (PCB 28, PCB 52, PCB 101, PCB 138, PCB 153, PCB 180 and PCB 209) were significantly higher in the cancer group compared with the control group. PCBs were detected in 93.98 % of the cancer cases and 93.75 % of the normal subjects. Mean level of ΣPCBs was found significantly elevated in the cancer group (2.711 mg/kg) compared with the control group (0.536 mg/kg). PCB 52 was the most prevalent chemical with a mean level of 2.044 mg/kg in the cancer group and 0.134 mg/kg in the control group. It was observed that concentrations of PCBs increased linearly with the increasing age of the cancer patients. On the other hand, OCPs were detected in 97.59 % of the cancer cases and 93.75 % of the normal subjects. Mean concentration of total OCPs was found elevated in the cancer group (0.611 mg/kg) compared with the control group (0.321mg/kg). Endosulfan was the highest prevalent OCP with mean concentration of 0.214 mg/kg in the cancer group and 0.170 mg/kg in the control group. DDT metabolite - 4,4-DDE was found significantly elevated in the cancer group with a mean level of 0.131 mg/kg compared to the control group (0.020 mg/kg). Highest mean concentration of ΣPCBs was found in cases of the female genital system while highest mean of ΣOCPs was detected in the breast cancer cases. Significantly elevated level of cf-DNA was detected in the cancer group with a mean value of 5584.2 ng/μl compared with control group (1758.8 ng/μl). Detection of cf-DNA was 56.45 % in the cancer group and 36.84 % in the control group. Mean cholesterol level was found significantly diminished in the cancer group (116±22 mg/dl) compared with the control group (158.7±33 mg/dl). ALP activity was found elevated in the cancer cases with a mean level of 125±48 U/L compared with the normal counterparts (109±28 U/L). Mean total protein contents were found reduced in the cancer cases (6.2±0.58 g/dl) compared to normal subjects (7.2±1 g/dl). Cases having higher residues of PCBs and to some extent of OCPs has shown elevated concentrations of cf-DNA, comparatively higher levels of cholesterol and ALP and reduced total protein contents. In light of the obtained results, it has concluded that PCBs and OCPs are positively associated with the etiology of cancer. Moreover, PCBs and OCPs adversely affect other biological parameters of the body which may ultimately result into various cancers in humans.