Medicinal use of plants is as old as the history of mankind. Medicinal plants have the ability to produce valuable classes of compounds with interesting bioactivities. Due to increasing side effects of synthetic drugs, use of medicinal plants is increasing day by day. Pakistan has about 6000 species of higher plants, of which about 700 species are commonly used for medicinal purposes. Taxonomic, Phytochemical and Biological Screening of Some Selected Medicinal Plants of Lesser Himalaya Pakistan was carried out to determine the accurate taxonomic description, complete phytochemical profile and bioactivity of selected medicinal plants. Four medicinal plants, Spermadictyon suaveolens, Reinwardtia indica, Xylosma longifolium and Celtis eriocarpa were selected based upon ethnomedicinal importance and their least phytochemical exploration. Selected plants were analyzed for taxonomic evaluation, qualitative and quantitative phytochemical screening, proximate analysis, biological activities and LC-MS profiling. R. indica was also analyzed for isolation of phytochemicals. Selected plants were extracted with cold maceration and fractionated in n-Hexane fraction (HF), chloroform fraction (CF), ethyl acetate fraction (EF) and aqueous fraction (AF). Preliminary phytochemical tests revealed the presence of high amount of phenolics and flavonoids in all four plants. R. indica contains high amount of phenolic, flavonoid, tannin, terpenoids and low amounts of alkaloids and saponins. C. eriocarpa contains high amount of phenolic, flavonoid, tannin and terpenoids, while low amount of saponins. S. suaveolens contains high amount of phenolics, flavonoids and tannins. X. 1 2 longifolium contains high amount of phenolics, flavonoids, alkaloids and tannins, while low amount of terpenoids and saponins. Proximate analysis revealed that X. longifolium contains high amount of dry matter (90.30±0.14), low moisture content (9.70±0.14), high fiber, carbohydrates and nutritive values (353.66±0.70). R. indica and X. longifolium showed higher total phenolic contents. High total flavonoid content was found in crude methanolic extract (65.90±1.00) and ethyl acetate fraction (88.01±1.33) of R. indica. Total flavonoid content is also higher in chloroform (74.81±1.58) and ethyl acetate fraction (79.84±0.80) of X. longifolium. R. indica showed higher antioxidant, cytotoxic and antitumor activities in crude methanolic extract, chloroform and ethyl acetate fractions. The highest DPPH EC50 value was exhibited by ethyl acetate fraction (119.64±0.58) followed by crude methanolic extract (209.00±1.53). Chloroform fraction of R. indica also showed higher antitumor IC50 (255.90±0.97), followed by ethyl acetate fraction (297.663±0.91). C. eriocarpa showed higher antioxidant (324.81±2.60), cytotoxic (243.61±1.24) and antitumor (372.76.±0.34) activities only in ethyl acetate fraction. S. suaveolens revealed higher antioxidant activity in ethyl acetate fraction (149.15±0.59) followed by Chloroform fraction (208.13±1.78). X. longifolium showed higher antioxidant activity in crude methanolic extract (107.21±0.16) followed by ethyl acetate fraction (152.96±0.43) and chloroform fraction (187.95±0.26). X. longifolium also showed high cytotoxic and antitumor activity in EF (484.19±1.55 and 235.41±1.51 respectively). UHPLC-DAD-ESI-MS profiling yielded identification of 58 compounds in R. indica, 48 compounds in C. eriocarpa, 50 compounds in S. suaveolens and 60 compounds in X. longifolium. LC-MS profile and bioactivity revealed that bioactivity 3 of R. indica extracts is mainly due to phenolic acids and flavones derivates, C. eriocarpa is due to flavones derivatives, S. suaveolens is due to phenolic acids, Quercetin and luteolin derivatives, while X. longifolium is due to Quercetin, naringenin and apigenin derivatives. UHPLC-MS profile also revealed that S. suaveolens and X. longifolium are rich in Quercetin derivatives. Based upon promising results of bioactivities of R. indica it was selected for compound isolation. Two compounds, Diferuloyl sinapic acid and Esculin were isolated for the first time from R. indica. The present research was very helpful in determining the underlying phytochemical profile and bioactivity correlation of selected medicinal plants.
چلے چلو کہ منزل ابھی نہیں آئی نحمدہ ونصلی علی رسولہ الکریم امّا بعد فاعوذ بااللہ من الشیطن الرجیم بسم اللہ الرحمن الرحیم معزز اسا تذہ کرام اور میرے ہم مکتب شاہینو! آج مجھے جس موضوع پر لب کشائی کا موقع مل رہا ہے وہ ہے:’’چلے چلو کہ منزل ابھی نہیں آئی ‘‘ صدرِذی وقار! منزل کے حصول کے لیے جدوجہد ہر ذی روح کی خواہش رہی ہے، ہر کس و ناکس اس کے لئے کدوکاوش کرتا ہے، اس کی زندگی کا ہر لمحہ حصولِ منزل کے لئے وقف ہوتا ہے، ہمہ قسم لوگ شبانہ روز اس مقصد کے حصول کی خاطر کوشاں رہتے ہیں ، حصول منزل میں ہر آنے والی رکاوٹوں کو ختم کرنے کے درپے ہوتے ہیں، اور پھر یونہی ان کے لمحاتِ زیست گزرتے رہتے ہیں۔ جنابِ صدر! حشرات الارض سے لے کر انسان تک ہر ایک اپنی منزل کی طرف گامزن ہے، ہر ایک کی اپنی ایک منزل ہے، مورومگس کی منزل اور ہے، گل لالہ کی منزل اور ہے، جوئے نغمہ خواں کی منزل اور ہے، حر یرو پر نیاں کی منزل اور ہے، زمین پر رینگنے والی مخلوق کی منزل اور ہے، گل لالہ کے گردبھنبھنانے والی شہد کی مکھی کی منزل اور ہے، غلاظت پر چکر لگانے والی مکھی کی منزل اور ہے۔ صدرِ محترم! گلستان میں عندلیب خوش الحان کی منزل اور ہے، برگد کے درخت پر موجود بوم کی منزل اور ہے، آبادی میں شجر سایہ دار کی منزل اور ہے، ویرانے میں خشک تنے والے درخت کی منزل اور ہے، فضاء میں محو پرواز عقاب وشاہین کی منزل اور ہے ،مُردار کے گرد چکر لگانے والی گدھ کی منزل اور ہے۔ پرواز ہے دونوں کی اسی ایک فضا میں کرگس کا جہاں اور ہے شاہیں کا جہاں اور جنابِ صدر!
Until recently, Gwadar has always been mentioned as a small insignificant fishing town. Very little was known about its history and potential to the outside world. However, at the turn of the 21st century Gwadar became the focus of attention globally. The development of a deep seaport with China’s assistance and prospects of connecting China and Central Asia through Pakistan to the North Arabian Sea has brought this small, insignificant fishing town to international recognition. But history reveals that this region has always played a vital role since primeval times due to its geostrategic and geographical location. From Alexander’s retreat to Pakistan’s reclaiming Gwadar, it has always reminded its significance at different times in diverse manners. This paper aims to bring into light the historical journey of Gwadar, which usually remained unknown or were either considered trivial by historians. Gwadar became an important chapter in the Belt and Road initiative proposed by the Chinese president. It will link China, South Asia, Middle East, Central Asia, Africa and Europe through a network of land and sea routes and ports will contribute significantly to the progress and prosperity of Gwadar in Pakistan, China and the entire region.
The objective of this research is to understand the importance of exchange relationships between a leader and his subordinates aimed at improving performance in the universities of Islamabad. Data was gathered by means of questionnaires from a sample of 358 employees (academic and non-academic) and was examined using Statistical Package for Social Sciences (SPSS 22). The results show that both task and contextual performance have a positive relationship with LMX and should be given due consideration in managing human resources in order to achieve organizational objectives effectively. Results indicate that OC do not moderate the relationship between LMX and TP and between LMX and CP. Results also indicate that OC acts as a mediator between LMX and TP and also between LMX and CP. Conclusions of the study have several important theoretical and practical implications.