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Home > the Analytical Applications of Immobilized I Soluble Enzymes for the Determination of Adenosine 5·Triphosphale Based on Bioluminescence and Chemiluminescence Detection System

the Analytical Applications of Immobilized I Soluble Enzymes for the Determination of Adenosine 5·Triphosphale Based on Bioluminescence and Chemiluminescence Detection System

Thesis Info

Access Option

External Link

Author

Mohammad Anwar

Program

PhD

Institute

University of Balochistan

City

Quetta

Province

Balochistan

Country

Pakistan

Thesis Completing Year

2005

Thesis Completion Status

Completed

Subject

Chemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/6602/1/3357H.pdf

Added

2021-02-17 19:49:13

Modified

2023-01-08 08:29:43

ARI ID

1676727566104

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نظر کا دھوکا

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طاہر طیب

دروازے پر دستک ہوئی۔۔۔ وے بشیرے  !او جا ویکھ ۔۔کون آیا ہے باپ نے آواز لگائی۔۔جی ابا!۔۔ آیا بھئی آیا۔ ۔ ۔   دروازہ کھولا تو  سامنے خلیفہ کھڑا تھا۔۔۔ جی سائیں خلیفہ  بولیں۔۔۔  اس نے پیغام دیا  کہ جمعرات کو  پیر سائیں  آئیں  گے۔۔ یہ کہہ کر وہ چلا گیا۔۔۔بشیردروازہ بند کر کے آیا اور بولا  ۔۔ابا جی ۔۔۔ پیر سائیں آئیں گے  خلیفہ نے بتایا ۔۔۔ باپ نے پوچھا کب ۔۔۔ جمعرات کو ۔۔۔باپ نے سب گھر والوں کو  جمع کیا۔۔۔او نصیرے۔۔وزیرے۔ ۔۔ سب ادھر آ جاؤ۔۔۔۔سب آگئے۔۔۔پیر سائیں جمعرات کو ہمارے مہمان ہیں۔

ابا جی  پیر سائیں تو پہلے فصل  کے پکنے پر آتے  تھے ۔۔۔اس بار اتنی جلدی۔۔۔وزیرے نے حیرت سے پوچھا۔۔۔او وزیر ے ، آ! ۔۔،تو اپنی  بک بک بند کر ۔۔۔ پیر سائیں کی مرضی جب آئیں۔۔۔ مالک ہیں ۔۔۔وہ ہمارے ۔۔۔ ابا نے غصے سے کہا!۔۔۔سب کو سانپ سونگھ گیا ہو جیسے۔

وہ بھی کیا دن تھے  کہ جب بڑے  بولتے تو باقی سب  چپ کرکے سنتے ۔۔ اب تو بڑے اس انتظار میں ہوتے ہیں کہ کب چھوٹوں کاموڈ  ٹھیک ہو تو وہ بولیں ۔۔۔۔ابا نے اٹھتے ہوئے کہا ۔۔۔۔ ہاں ان کے حقے پانی کا خاص خیال رکھنا ہے۔۔ ۔ میں   ان کے لیے کچھ نذرانے کا بندوبست کرتا ہوں ۔۔۔۔صبح ہوئی تو ابا نے گائے کھولی اور اسے لے کر جانے لگا۔۔ ۔ وزیرے نے کہا ۔۔۔ابا کہا ں جا رہے ہو ۔۔ ابا نے جواب دیا ۔اسے بیجنے ۔۔۔ پیر سائیں کو نذرانہ بھی تو دینا ہے۔۔ ۔ میں  چلوں ۔۔۔ابا کی آنکھ کو  دیکھ کر۔۔۔ وزیراسمجھ گیا کہ  جواب ۔۔۔نہیں کا ہے ۔

 جاتے جاتے ابا نے کہا ! ۔۔اور وہ جو دو تین...

OCCURRENCE OF LOWER EXTREMITY MUSCULOSKELETAL INJURIES DURING THE LOCKDOWN IN ATHLETES

Background of the Study: Lockdown was implemented worldwide to limit the spread of COVID-19. This sudden implementation of lockdown causes significant lifestyle changes for every individual. Along with the general population, it also has psychological, behavioral, and physical consequences on athletes. The study objective is to determine the occurrence of lower extremity musculoskeletal injuries during the COVID-19 lockdown in athletes. Methodology: Retrospective cross-sectional study design was used, and participants were recruited by a non-probability convenient sampling technique. A sample size of 147 was taken as calculated by the Raosoft software, and the study was completed 6 months. Both male and female athletes between the age group of 18-35 years, participants who did not participate in any official training session during the lockdown and registered at domestic level for at least 2 years were recruited from Pakistan Sports Board and Wapda Sports Complex Lahore. Data was collected using a semi-structured questionnaire. Nordic Musculoskeletal Questionnaire was used to identify the problematic painful areas of body. Data entry, analysis, and interpretation were done by using SPSS software version 22.0. Results: The mean age and BMI of participants were 25.6531±4.49 (years) and 23.28±3.24 (kg/m2) respectively. From the total, 39.5% of participants reported lower extremity musculoskeletal injuries. And most reported problematic areas include lower back and knee. 75% of participants continue to do workouts at home as a prevention strategy against injury occurrence. Conclusion: This concluded that the occurrence of lower extremity musculoskeletal injuries during the lockdown was moderate.

Efficacy of Some Indigenous Medicinal Plants to Control Antinematicidal-Resistant Haemonchus Contortus in Sheep

Antinematicidal resistance has been arising among the gastrointestinal nematodes, primarily Haemonchus (H.) contortus in small ruminants to the commercialized broad- spectrum synthetic chemotherapeutics in Pakistan and other countries of the world. Therefore, the current study, which was carried out on the Karakul breed of sheep in Angora Goat Farm - Rakh Khairewala, Layyah District (Pakistan) , began with the intention of finding out the rampancy of resistance among H. contortus populations in addition to assessing some native medicinal plant extracts for their potency to control the resistant parasite. The experimental animals were allocated to 16 groups (n=15) followed by the demonstration of haemonchosis percentage (˃90%) among other nematodiasis. Three groups were allotted for detection of resistance towards oxfendazole (OXF), levamisole (LEV) HCL and ivermectin (IVM) plus one group for control. The faecal egg count reduction test (FECRT) revealed prevalence of resistance to the above drugs with varying levels. Pursuant to the RESO Computer Programme, the calculated FECR% was -56, 75 and78, whilst the lower confidence interval was -311, 38 and 42 for OXF, LEV and IVM, respectively. The LC50 value for oxfendazole, estimated via probit analysis, was 1.86 μg ml‫ 1 ־‬after performing egg hatch assay (EHA). For studies on phytotherapeutics, 12 groups (n=15) were allotted. However, the same control group was used because, statistically, all the groups are located within the same population. The calculated FECR% for effective crude aqueous-methanol extracts of the tested plants was 94.59, 88.6, 87.5, 85.81 and 85.14 for combined Nicotiana (N.) tabacum leaves and Azadirachta (A.) indica seed kernels, high dose of N. tabacum, low dose of N. tabacum, high dose of combined N. tabacum and Vernonia (V.) anthelmintica seeds, and A. indica, respectively. LC50 values, determined through probit analysis after conducting EHA, were 0.523, 0.566, 1.169, 1.217, 3.138, and 5.95 μg ml‫ 1 ־‬for combined N. tabacum and A. indica, N. tabacum, A. indica, combined N. tabacum and V. anthelmintica, combined V. anthelmintica and A. indica, and V. anthelmintica respectively. The adult motility test revealed high adulticidal potency of solitary N. tabacum and combined N. tabacum and A. indica which killed 100% of worms after six hours of exposure at the concentration of 25 mg ml‫ ,1 ־‬whilst the others were less effective. No synergy, additive effect and antagonism were observed.