Search or add a thesis

Advanced Search (Beta)
Home > The Potential of Selected Flavonoids Against Methicillin Resistant Staphylococcus Aureus

The Potential of Selected Flavonoids Against Methicillin Resistant Staphylococcus Aureus

Thesis Info

Access Option

External Link

Author

Muhammad Usman Amin

Program

PhD

Institute

Kohat University of Science and Technology

City

Kohat

Province

KPK

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/7323/1/Muhammad_Usman_Amin_Microbiology_2016_HSR_KUST_Kohat_03.05.2017.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727590696

Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.

Similar


As a result of developing resistance the antibiotics became ineffective and the microbes including bacteria, viruses, parasites and fungi became able to counterattack against antibiotics. The basic cause of antibiotic or antimicrobial resistance is because of too much use of antibiotics. The impending post antibiotic period is becoming a threat for the both current and forthcoming advances in medical science. Both the rise in the bacterial resistant strains and insufficient availability of novel antibiotics are the major causes of antibiotic resistance. As a result of which the chances for the treatment of various infections have decreased significantly which have given birth to various complications for the patients. Among the resistant microbes, MRSA is currently a very hot issue globally for the last sixty years. This has been responsible for nosocomial infections and has been known to cause threats to life. The resistance against penicillins belongs to β- lactams antibiotics, started soon after a few years of its discovery. Because of rising issue of MRSA, a study was designed to determine the effect of flavonoids on antibiotics which have developed resistance against methicillin resistant Staphylococcus aureus (MRSA). The flavonoids selected for this research were Rutin (R), Morin (M), Qurecetin (Q) and Luteolin (L). The antibiotics included were AMP, AMO, CEF, CET, VAN, ME, CEPH, ERY, IMP, CIP, LEV and SULP-TRI. In this study about 100 MRSA clinical isolates and MRSA ATCC No. 43300 were used. The flavonoids were used alone, in combinations and along with the selected antibiotics. The concentrations of flavonoids and in combination with antibiotics effective against clinical isolates and standard strain were determined through the antibiotic sensitivity assays. The flavonoids, found effective individually against the clinical isolates and standard strain were Q and L. The combination of flavonoids used which have more pronounced effects than the individual ones were Q+L, R+M and Q+M+R. These flavonoids were then used in combination with all the selected antibiotics. The results revealed the qurecetin and luteolin used alone and in combination along with antibiotics have been found to increase the efficacy of AMP, CET, CEPH, ME and IMP against clinical isolates and the standard strain. All these antibiotics alone were having no effect against MRSA isolates except IMP.M+R used in combination with amoxicillin, ceftriaxone, cephradine, methicillin and imipenem, have positive effect on the antibacterial activity of test antibiotics, however, querecetin-morin-rutin combination was found to have more enhancing effects on the test antibiotics (AMP, AMO, xiv CET, CEPH, ME and IMP) activity against the standard and MRSA clinical isolates than the rutin-morin combination. The minimum inhibitory concentrations (MIC’s) of flavonoids were determined. Then in combination with antibiotics, MIC’s of flavonoids were determined as well. A reduction was observed in the MIC’s of flavonoids when combined with test antibiotics. Similarly MIC’s of antibiotics alone and in combination with flavonoids were also determined. A profound decrease was demonstrated in the minimum inhibitory concentration of antibiotics. The MIC’s reduction of antibiotics and flavonoids in combination with each other proves that both of them were increasing each other activity against the bacteria under study. Fractional inhibitory indices (FICI) were measured to find out whether there was synergism or additive relationship between the antibiotics and flavonoids. The results revealed that in all the cases additive relationship existed between the antibiotics and flavonoids except Q+M+R+CEPH, Q+M+R+CET, Q+M+R+IMP, Q+M+R+MET, Q+L+CET and Q+L+IMP where synergism was observed. Then the interaction mechanism between the flavonoids and antibiotics was also determined by measuring the K+ loss caused by flavonoids, antibiotics and in combination with each other. After that mutagenic effect of flavonoids alone and in combination with antibiotics on MRSA was also detected by PCR and sequencing. No mutations were observed. The resistant antibiotics become active against MRSA when combined with flavonoids. This was proved by determining the MIC’s of flavonoids alone and in combination with antibiotics. This revealed significant reduction in the MIC’s. Similarly, the MIC’s of antibiotics alone and in combination with flavonoids was also determined showing a profound reduction in the MIC’s of antibiotics. This proved that both the flavonoids and antibiotics were increasing each other’s activity. The FICI indicated an additive relationship between them. While the potassium loss measurement was the indicative of cell wall damage, which was greatest when flavonoids and antibiotics were used together.
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

ایاز سموں

ایاز سموں

پیپلز پارٹی کا یہ جیالہ 1959ء میں سندھی خاندان میں پیدا ہو ا ۔شروع شروع میں سندھی نیشنلسٹ گروپ میں شامل ہوا ۔1977ء میں PSFمیں شمولیت اختیار کی ۔دہشت گردی کے الزام میں گرفتار ہو کر جیل پہنچ گیا ۔1985ء میں فوجی عدالت نے سزائے موت سنائی اور26جون کو اسے پھانسی دے دی گئی ۔پھانسی کے وقت اس کی عمر 26سال تھی ۔

 

 

Analisis Strategi Pemasaran Marketing Mix untuk Meningkatkan Penjualan Pada D’Besto Fried Chicken Pekanbaru

Marketing strategy is an effort to market a product, both in the form of goods and services, using certain plans and tactics to increase sales volume. One of the business development strategies is the implementation of a marketing mix strategy. Marketing is one of the most important factors in the continuity of a business, so it is very important for business people to pay attention to the marketing mix in their business. The purpose of this study was to determine how D'besto Fried Chicken Pekanbaru applies sales promotion. The data analysis technique used is market mix analysis. The marketing mix variables studied were product, price, place and promotion. The results of this study indicate that consumer decisions in purchasing D'besto Fried Chicken Pekanbaru are strategic location selection and products that are acceptable to the public. The recommendation of this research is that D'besto Fried Chicken Pekanbaru products should be more diverse and innovative in terms of packaging and online marketing and improve brand quality.

Nutritional Assessment of Some Neglected and Underutilized Vegetables Wildly Grown in Sindh

comparison of nutritional value of nontraditional with commercial vegetables in Sindh. The questionnaire survey methodology was used to collect the data on the utilization and consumption of nontraditional and commercial vegetables in Mirpurkhas of Sindh province, Pakistan in 2014. On the basis of survey spinach, horse radish tree flowers, lambs quarter and gram leaves were collected in January, 2014 whereas, amaranthus was collected in the months of July-August, 2014 from district Mirpurkhas, packed with proper labelling and brought to the Institute of Food Sciences and Technology, Sindh Agriculture University Tandojam for processing and nutritional analysis. The edible parts of vegetables were washed and divided into five sets namely control, boiled, cooked, thermally dehydrated and shade dried. The data of survey showed that gram leaves was the most popular nontraditional vegetable eaten frequent or occasionally by 82% respondents only 18% respondents never tasted or do not know this vegetable. Next vegetables which majority of respondent never tasted or did not know included amaranthus and lambs quarter. About 62% respondents never tasted or do not know horse radish tree flowers as vegetable while 38% respondents answered they eat occasionally. The nontraditional (lambs quarter, horse radish tree flowers, gram leaves, amaranthus) and commercial (spinach) vegetables were analyzed for their nutritive, mineral, vitamin, phytochemical and chlorophyll composition. The highest moisture content (92.66%) was found in spinach under boiled method followed by 88.760% moisture content in the same vegetable at fresh (control). Maximum ash content (16.15%) in horse radish tree flowers followed by 10.56% ash content in amaranthus under thermally dehydration. Protein content was found greater (7.56%) in gram leaves under thermal dehydration method. However, minimum protein of 1.04% was found in spinach under boiling method. The maximum value of 3.85% in horse radish tree flowers under cooking method while minimum fat content i.e. 0.85 and 0.75% was found in spinach and lambs quarter, respectively at boiling method. The highest value (13.35%) of fiber was obtained in thermally dried sample of horse radish tree flowers whereas the lowest value was recorded in boiled sample of spinach. However, higher carbohydrate (68.62%) content was found in lambs quarter at shade drying. The nontraditional vegetables also contain organic acids (e.g. lactic acid, citric acid, acetic acid, tartaric acid) in all the selected vegetables. The nontraditional and commercial vegetables were also recorded with significant amount of vitamins and phytochemicals. The energy value was found lowest in fresh spinach (38.35 Kcal 100g-1) hence was also detected lowered in other processing methods as compared to nontraditional vegetables. The results of the sensory evaluation of the uncooked and cooked samples in present study revealed that in uncooked samples, horse radish tree flowers obtained highest scores in appearance, color, odor, texture, taste, overall acceptability and purchase i.e. 4.90, 4.70, 4.00, 3.90, 3.50, 3.80 and 3.80. While in traditionally cooked samples lambs quarter and gram leaves retained original color and thus obtained the highest scores in appearance and taste i.e. 3.70, 3.90 and 3.70, 3.50, respectively. Acceptability study by hedonic scoring showed that nontraditional vegetables (horse radish tree flowers, lambs quarter, and gram leaves and amaranthus) made by traditional cooking were most acceptable as compared with commercial vegetable (spinach). These nontraditional vegetables when consumed in cooked form could also be a good source of nutrients. Principal component analysis revealed that the first seven principal components explained about 94.79% of the total variability in the observed parameters. Moisture, total solid, ash, fiber, carbohydrate, nitrogen free extract, energy value, acetic acid, citric acid, oxalic acid, tartaric acid, copper, iron, zinc, manganese, calcium, sodium and potassium resulted the most effective variables for the first principal component. Saponins, flavinoids, phenol and vitamin B3 were major contributors to second principal component, while tannins content was useful to define the third principal component. It was concluded that the nutrient and bioactive contents obtained from selected vegetables seem to suggest that the vegetables have high potential to contribute to the nutritional and health status of local as well as urban communities in Sindh Pakistan. Their use in the communities should therefore, be promoted. Taking into account the amount of nutrient and bioactive content in the selected nontraditional vegetable, these plants could be valuable and important contributor to the diets of the people in Sindh, Pakistan.