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Home > Thermostable Kinases from Pyrobaculum Calidifontis: Cloning and Characterization

Thermostable Kinases from Pyrobaculum Calidifontis: Cloning and Characterization

Thesis Info

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Author

Bibi, Tahira

Program

PhD

Institute

University of the Punjab

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Biological Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12792/1/Tahira%20Bibi_Biological%20Sci_2017_UoPunjab_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727607551

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Analysis of the genome sequence of Pyrobaculum calidifontis revealed the presence of an open reading frame Pcal_1127 annotated as ribose-5-phosphate pyrophosphokinase. To examine the properties of Pcal_1127 the coding gene was cloned, expressed in Escherichia coli, and the purified gene product was characterized. Pcal_1127 exhibited higher activity when ATP was replaced by dATP as pyrophosphate donor. Phosphate and EDTA activated the enzyme activity and equivalent amount of activity was detected with ATP and dATP in their presence. Recombinant Pcal_1127 could utilize all the four nucleotides as pyrophosphate donors with a marked preference for ATP. Optimum temperature and pH for the enzyme activity were 55 °C and 10.5, respectively. A unique feature of Pcal_1127 was its stability against temperature as well as denaturants. Pcal_1127 exhibited more than 95 % residual activity after heating for 4 h at 90 °C and a half-life of 15 min in the boiling water. The enzyme activity was not affected by the presence of 8 M urea or 4 M guanidinium chloride. Pcal_1127 was a highly efficient enzyme with a catalytic efficiency of 5183 mM−1 s−1. These features make Pcal_1127, a novel and unique ribose-5-phosphate pyrophosphokinase.
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۳۱۔ انتظار

انتظار

ماں ۔۔۔ !تم نے کہا تھا

بابا آسماں پر سیر کو گئے ہیں

 کل آئیں گے

میں اس کل ہی کے انتظار میں بسمل ہو گیا ہوں

اے ماں! تو بھی کہہ بابا سے

 ’’اب لو ٹ بھی آئیں ‘‘

ان کی انگلی تھامے بنا

اب چلنا مشکل ہو گیا ہے

غریب الحدیث پر موجود کتابوں کے اسالیب کا مطالعہ اور ان کے مناہج کا تقابلی جائزہ

Allah the elevated bestowed on prophet Muhammad SAW two basic sources of guidance for Muslim Ummah, The holy Quran and Hadith. Due to this significance of Hadith, Muslims have invented more than five hundred sciences related to Hadith. One of these sciences is Ilm Garb ul Hadith. Sheikh Moaamer bin muthana was the first scholar who has written a book on this topic.  From then on Muslim scholars have researched a lot in this regard. Dozens of scholars spent their time and wealth on it. According to the author of Moaajm ul mua’ajam more than 90 books on the topic have been published but eight of them gained much publicity and famous hood among them. Abu Ubaida, Abu Adnan, Abu Ubaida Qasim bin Salam, Ibrahim bin Ishaq Al Harbi, Abu Ubaida Ahmed bin Muhammad Alhervi, Ibn Jauzi, Muhammad bin Atheer Aljazree, Zemakhsharee. The following article consists of a brief introduction of Ilm Gharib ul Hadith along with a brief history of research about it. Then the eight famous books on Gharib ul Hadith and there way of research are examined in brief along with examples. At last a comparative study of the work done by these eight scholars is given in order to explore the differences and similarities among them.

عبدالحلیم شرر کی غیر افسانوی نثر تحقیقی و تنقیدی جائزہ

This thesis has been divided into seven chapters. Definition and difference between novel and non-fiction prose has been deliberated upon in the first chapter. Story, novel, drama and tale are part of non-fiction prose. And remaining all kinds like, Seerat, biography, subjects, diction thesis, history, journalism, books etc. are included in non-fiction prose. In retrospect, political and social aspects have also been highlighted so as to ascertain the events and circumstances that led Sharer to write non-fiction prose. Certain movements have been mentioned to identify their effects on Sharer's skills. Topics like beginning of Sharer's literary life and his abilities are also included in the first chapter. Second chapter deals with Abdul Haleem Sharer as a biographer. Commencement and evolution of biography and research and critical analysis of Sharer's biography has been carried out in this chapter. Third chapter discusses Sharer as an essay, composition and dialogue writer. Beginning and evolution of essay and composition and Sharer's relating skills have been researched and analyzed. Distinctiveness in essay writing, dialogue writing, composition writing, the topics and writing style of Sharer has also been evaluated. Sharer has been a great historian and critics. A study of history writing is an art. Motives and objectives of Abdul Haleem Sharer's historical writings and history books written by him have been analyzed in depth. His rank and standing amongst the contemporary historians have also been discussed in the fourth chapter. Fifth chapter relates to Sharer as a journalist. Beginning and evaluation of journalism, Sharer's life as a journalist and research and analysis of various papers & periodicals has been carried out. Discussion on Sharer's standing in Urdu journalism has been discussed here. Sharer's style and protocol as a book writer is quite persuasive. Evolution of letter writing has been briefly touched upon in chapter six. Sharer's letters, topics of effects letters on his personality in the light of these letters, its significance etc. have been included in this chapter. Seventh, the last chapter summarizes Sharer's contribution towards Urdu literature. Beyond doubt, Sharer's contributions can be termed as consecration in the realm of literary circles.