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Home > Topical Creams for Acne and Melasma: In-Vitro and In-Vivo Investigations of Active Botanical Extract Formulations

Topical Creams for Acne and Melasma: In-Vitro and In-Vivo Investigations of Active Botanical Extract Formulations

Thesis Info

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Author

Khan, Barkat Ali

Program

PhD

Institute

Islamia Collage Peshawar

City

Peshawar

Province

KPK

Country

Pakistan

Thesis Completing Year

2013

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/2654/1/2664S.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727617174

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Background : Melasma is a common facial pigmentary disorder. A multifactorial contribution is suggested in the etiology of melasma and ultraviolet (UV) irradiation plays an important role in melasma. Plants have been used worldwide as medicine and have been reported to show anti-melasma activities. Cutaneous infections especially acne of the skin are one of the often faced dermatological diseases worldwide. Topical therapy is an attractive choice for the treatment of the cutaneous infections due to its advantageous such as targeting of drugs to the site of infection and reduction of the risk of systemic side effects. Materials and Methods : Methanolic extract of Hippophae rhamnoides and Cassia fistula were effectively extracted and phytochemically screened. The antioxidant activities of the plant extracts were measured by Nitric oxide radical scavenging assay, DPPH radical scavenging assay, Hydroxyl radical scavenging assay and Superoxide radical scavenging assay. Total phenolic contents observed spectophotometrically. Stable creams, F1 (Hippophae rhamnoides extract), F2 (Cassia fistula extract) and F3 (placebo) of o/w type were prepared using Paraffin oil as oily phase, Tween 80 and Span 20 as the emulsifiers while distilled water as the aqueous phase. 5% crude plant extract was used in F1 and F2 while F3 was without the plant extract. All these creams were kept at four different storage conditions i.e., 8oC, 25oC, 40oC and 40oC with 75% Relative Humidity for a period of two months to evaluate their stability characteristics. Human volunteers were used for in-vivo assessment of F1, F2 and F3 to evaluate their effects on human skin. Different skin parameters like Skin Melanin (for melasma effects), Skin Sebum contents (for acne effects), Skin Trans epidermal water loss (TEWL) and Stratum corneum water contents (for moisturizing effects) and Surface evaluation of living skin (SELS) parameters related to skin aging were measured at different time intervals for three months. For acne and melasma effects, instrumental readings, visual observation as well as photographs were taken while other parameters were observed only by instrumental readings. XIX The results obtained were compared with the zero hour readings and the statistical tools of ANOVA (Kruskal–Wallis tests) and Dunnett Multiple comparisons post test were used in the statistical analysis to evaluate the changes produced. Results : Both plants extracts showed appreciable antioxidant potential and statistically insignificant differences (P>0.05) were observed among the triplicates in different systems of antioxidant activity evaluation. The antioxidant activities were more when methanol was used as extracting solvent and maceration as extraction method. The expected pharmaceutical stability of emulsions was achieved from 2 months in-vitro study period. Results of an electrical conductivity confirmed that an outer phase is water. A significant (P≤0.05) decrease in the level of melanin was produced by F1 and F2. The difference between pre and post-treatment levels of melanin was statistically significant (P = 0.05). In case of skin sebum contents, a significant (P ≤ 0.05) decrease was observed for both F1 and F2. The difference between pre and post-treatment levels of sebum contents was statistically significant (P=0.004). F1 and F2 showed significant (p<0.0001) effects on SELS parameters i.e., skin wrinkles (SEw), skin smoothness (SEsm), skin scaliness (SEsc) and skin roughness (SEr). The results were supported by 3D images of human skin after applications of respective creams. Stratum corneum water contents were increased and Trans epidermal water loss (TEWL) was decreased significantly (p<0.05) supporting the results of SELS parameters. Conclusions : As a conclusion creams with 5% plant extracts could be effective, safe and well-tolerated topical medications for melasma (hyperpigmentation), acne vulgaris and dry skin conditions.
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پروفیسرڈاکٹر سید محمد فاروق بخاری

ڈاکٹر سید محمد فاروق بخاری
مئی کے آخر یا جون کے شروع میں جناب شوکت حسین کینگ مدیر ماہنامہ الاعتقاد سری نگر کے ایک مکتوب سے یہ معلوم کر کے بڑا دُکھ اور سخت افسوس ہوا کہ ریاست کشمیر کے مشہور صاحبِ علم و قلم پروفیسر ڈاکٹر سید محمد فاروق بخاری طویل علالت کے بعد ۱۹؍ ذی الحجہ ۱۴۱۷؁ھ؍ ۲۷؍ اپریل ۱۹۹۷؁ء کو رحلت فرماگئے، یہ اطلاع خود ہی تاخیر سے ملی تھی اور باوجود کوشش کے جون کے معارف میں ان پر نوٹ شایع کرنے کی گنجائش نہیں نکلی۔
بخاری صاحب کی عمر ابھی زیادہ نہ تھی اور ان سے بڑی توقعات وابستہ تھیں مگر موت کا وقت معین ہے، اس میں تقدیم و تاخیر نہیں ہوتی، فاروق صاحب ۲۷؍ جون ۱۹۴۹؁ء کو پیدا ہوئے تھے، ان کا خاندان علمی، دینی اور روحانی فضیلت کا حامل تھا، ان کے والد بزرگوار مولانا سید محمد قاسم بخاری کو جو ابھی خدا کے فضل سے بقید حیات ہیں مولانا مفتی کفایت اﷲ دہلوی سے شرف تلمذ حاصل تھا۔ موصوف انجمن تبلیغ الاسلام جموں و کشمیر کے صدر اور حنفی عربی کالج سری نگر کے بانی مہتمم ہیں، کشمیر کے اس بخاری خانوادے کانسبی سلسلہ حضرت شیخ عبدالقادر جیلانی سے ملتا ہے مولانا سید عطاء اﷲ شاہ بخاری بھی اسی خاندان کے چشم و چراغ تھے۔
ڈاکٹر مولوی فاروق بخاری کی تعلیم کی ابتدا کشمیر میں ہوئی اور کشمیر یونیورسٹی ہی سے انہوں نے مولوی فاضل کیا، لیکن عربی میں ایم۔اے اور پی۔ایچ۔ڈی کی ڈگری علی گڑھ مسلم یونیورسٹی سے حاصل کی۔ موصوف کا خاص مشغلہ درس و تدریس تھا اور اب وہ امر سنگھ کالج سری نگر میں شعبۂ عربی کے ہیڈ آف ڈپارٹمنٹ تھے۔ لیکن تصنیف و تالیف کا بھی ان کو اچھا ملکہ تھا۔ کشمیر کی علمی، ادبی، ثقافتی اور مذہبی تاریخ ان کا خاص موضوع...

اسوہ  حسنہ اورمغربی ذہن کے اشکالات.استشراقی فکرکے تناظرمیں تحقیقی و تنقیدی جائزہ

Western mind always remains muddled to fathom the Islamic belief “Prophethood” but it is grand reality that the West has contributed a lot in “Sirah Writing” from 17th to the present age. Although, in primary literature, Western scholars have delineated their abhorrence in this regard, without any research analysis. They usually used to twist the facts and misinterpret the text in attempt to sustain their assumption under their own perception. In the present age, literary change is seen in Western Writings that scholars have admired categorically the demonstrative Personality of Mohammad (PBUH) as well as they have praised his attributes as authoritative leader, Reformer, Peace maker etc. It will discuss the changes which were seen in Western writings about Sirah and analysis the causes and facts accordingly. It will also throw light on the Western assumption about Prophet Muhammad (PBUH); which has been prevailed in their writings before 19th even changed now. Islam is religion of Peace and Prophet of Islam has sent as” Blessing for All the Universe” from God Almighty so the question is what are the motives to bring forward theses western suppositions in this regard and at what extent it will affect? It will be discussed through comparative analysis among the Sirah books by Western Scholars Maxim Rodinson, Karen Armstrong and Michael Cook.

Structure and Efficiency Analysis of Vegetable Production and Marketing in Sindh, Pakistan

Onion, tomato and chilies are most common and important kitchen items in Pakistan. The consumption of tomato and onion has high-income elasticity of demand, while the demand for chilies is almost inelastic. Thus, increased demand for tomato and onion is anticipated with economic development and urbanization. Further, the demand for these vegetables will also increase with population growth. Per capita consumption of vegetables in Pakistan is currently very low when compared to other countries of the region and world. People in upper income strata consume well above the national calculated average, while the bulk of rural population and large percentage of poorer strata among the urban population consume very few vegetables. For sustainability of vegetable enterprise, it is a prerequisite that the production and marketing system is profitable and efficient. Pakistan has unique but complex network of up to five or six intermediaries between the primary producer and the end user. The qualitative and quantitative analysis of vegetable production and marketing system determines the level and extent of production efficiency and market perfections. Realizing the importance of structure of vegetable enterprise and its efficiency and with a view to access the complexity of vegetable production and marketing for profitable production and efficient distribution system, the present study was designed and conducted. The main purpose was to conduct a detailed investigation of the present vegetable production and marketing system in Pakistan for analyzing production and marketing efficiency. For this study, primary data were collected from farmers by conducting surveys in three districts of Sindh, namely Hyderabad, Thatta and Mirpurkhas. Hyderabad was selected for onion crop, Thatta for tomato crop, and Mirpurkhas for chilies. Sixty farmers for each vegetable were randomly selected from these districts so the total sample size was 180 farmers for this study. Besides, 130 market intermediaries were also interviewed. Primary data were collected by survey method using a pre-tested questionnaire and secondary data were gathered from statistical bulletins. Time series panel data on market prices in four spatial markets of Pakistan for the selected vegetables was used to investigate long run market relationships. Similarly time series data of production of these vegetables was used for forecast estimates. A combination of analytical techniques including the measurement of growth rates of area and production of vegetables in Pakistan and across provinces, returns to scale analysis through using Cobb-Douglas production function, estimation of technical efficiency and technical efficiency rating across farms, marketing margins, market integration, and production forecast were used to assess the production and marketing system of onion, tomato and chilies in Sindh. Positive growth in area of vegetables in Pakistan was noted with higher growth of area in Punjab and Balochistan followed by North West Frontier and Sindh provinces, while the production of Punjab depicted a growth rate of 1.6 percent followed by Sindh (1.3%) and Balochistan (0.9%). The growth rate of NWFP in vegetable production was negative during 1986-87 to 2004-05. The growth rate of vegetable production and corresponding comparisons of area clearly indicated that the vegetable yields in Sindh had remained and grown higher than rest of the provinces of Pakistan. Cobb-Douglas production function was estimated to measure elasticity of production and the degree of returns to scale for onion, tomato and chilies producing farms in Hyderabad, Thatta and Mirpurkhas districts of Sindh. Coefficients of elasticity of production indicated significant contribution of land, (iv)labor and capital inputs in production of these crops. For returns to scale, the t-test was applied for testing the null hypothesis that degree of homogeneity equals 1. Null hypothesis was maintained at 5% significance level for each of onion, tomato and chilies crops. The results showed that the onion, tomato and chilies production exhibited constant returns to scale. These results indicated that if all inputs were increased proportionately, the output could be increased with the same proportion. The efficiency rating based on corrected intercept of the estimated production function indicated that a reasonably big number of farmers were using their inputs inefficiently. Thus, under the present standardized cultural practices, the vegetable growers seem to be operating at a lower level of production possibility curve in technological terms. The mean efficiency of chilies, tomato and onion was 0.83, 0.74 and 0.59 respectively. The results further revealed that chilies growers were more efficient when compared to the onion and tomato farmers, while, tomato growers were found more efficient than onion farmers. The relationships across marketing chains involved in the selected vegetable were studied by investigating marketing margins, distribution of costs and net returns across the functionaries. The results of price spread across marketing chain revealed that share of producer in consumer rupee was 58, 66 and 65 percent for onion, tomato and chilies respectively, while the rest goes to commission agents, wholesalers and retailers. The results further revealed that the average returns of retailer on capital employed were three times higher in onion business and almost two times higher in tomato and chilies. Spatial price relationship is an important indicator of overall market performance. In spatially integrated markets, competition among arbitragers usually ensures that a unique equilibrium is achieved since local prices in regional markets differ by no more than transportation and transaction costs. The results of empirical evaluation of spatial price linkages among four regional markets of Pakistan using monthly wholesale real prices of onion, tomato and chilies indicated that these markets were efficient as their speed of adjustment for reaching an equilibrium level was higher. The difference in per unit prices of onion, tomato and chilies was due to transaction and transportation cost, thereby confirming the spatial integration of regional vegetable markets in Pakistan. The results of unit root test for onion and tomato show that the production time series is represented as a random walk model with drift, which indicates that the shocks to production in a year have permanent effect on the level of future production, while the time series of chilies production showed a pure random process. The forecast results show that production of tomato and onion, respectively, is expected to grow by 12825 and 40226 tonnes per annum, which results in little growth in per capita production of these vegetables. The efficiency of long run relationship across regional vegetable markets confirmed that price information flow is adequate. The higher returns on capital employed by the market agents including wholesalers, and commission agents indicated a collusive oligopoly among market intermediaries and they were not operating as competitive and efficient institutions. The vegetable forecast results revealed that per capita onion and tomato production would maintain upto 2020, and chilies production would not decline upto 2020. The outcome of the present research was to suggest policy measures for government and to extend recommendations for further research. The results have revealed that the yields of vegetables in Sindh have grown at higher rate when compared to other provinces, perhaps due to higher demand based on the urbanizations. Sindh is major producer of chilies as it produces 75 percent of chilies in the country. Results reveal that other provinces have also started increasing its areas as well as production of chilies. Punjab province is also increasing onion production for reducing its dependence on Sindh and Balochistan provinces. As Sindh and Balochistan are relatively efficient in onion production, and Punjab province has neither comparative nor competitive advantage on Sindh and Balochistan provinces to produce onion. Hence, Ministry of Food, Agriculture and Livestock, (v)Government of Pakistan may intervene in giving guidelines for promoting efficient production of onion and optimum land use in Pakistan. Onion and chilies production is highly elastic to labor inputs, which indicates the significance of labor and their skills in production of these crops. Better vocational and skills training and technology transfer to laborers in onion and chilies production may help not only in increasing level of efficiency for producing these crops but it may change the steady state of production function by its upward movement. The vegetable production exhibited constant returns to scale and inefficiencies across farms; therefore, it was suggested that agricultural extension, support services, credit and technology transfer may be improved to increase output and generate exportable surpluses. It was also suggested that agrarian reforms be introduced including asset redistribution on selective basis to maximize the benefits of growth across households and reduce poverty through high value vegetable crops. Further inter-provincial trade may be promoted by developing road and other infrastructure in order to reduce transport and other transaction costs. It was further suggested that the Government may formulate an appropriate policy to invest in research and development for enhancing the yields of these vegetables. Major investment in supportive infrastructure was recommended to facilitate marketing and trade of vegetables including onion, tomato and chilies. Need was established for collaboration between private and public research and development programs to improve management practices, particularly the use of available technology for timely and efficient production. The analysis highlighted that farmers need to move the production upward and change the steady state of the function in order to attain the yield levels of experimental stations, which may, in turn, develop new production frontiers for vegetable production and quality enhancement. The results of this study indicated that the vegetable production system is partially efficient, while the marketing system is moderately efficient. An important role may, therefore, be envisioned for government as facilitator and promoter of an efficient production and marketing system. Government has also a role to play for stabilizing prices of perishable commodities to protect producers in the short run, and establish market infrastructures including cool chains as a long term solution. Government should also give incentives for growth and promotion of input industry required for onion, tomato and chilies production and to industries like packaging, processing, transportation and storage to promote trade along with employment. Profits of middlemen may also be rationalized through regulation and selective control. Government role needs to be invoked, wherever necessary, to remove market imperfections in the interest of producer and consumer. Pakistan needs to focus on vegetable exports particularly onion, tomato and chilies. The study pinpointed the need for maintaining time series data for different indicator so as to create a reliable management information system for planning, appraisal, implementation, monitoring and evaluation of production, marketing, imports, and exports for vegetables in the long run." xml:lang="en_US