Search or add a thesis

Advanced Search (Beta)
Home > Trace Metals in Scalp Hair, Plasma and Tissues As an Index of Cancer

Trace Metals in Scalp Hair, Plasma and Tissues As an Index of Cancer

Thesis Info

Access Option

External Link

Author

Qaisara. Muhammad Ali

Program

PhD

Institute

Quaid-I-Azam University

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2008

Thesis Completion Status

Completed

Subject

Chemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/3188/1/2468.pdf

Added

2021-02-17 19:49:13

Modified

2023-01-07 22:59:42

ARI ID

1676727625764

Similar


Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

سید عنایت حسین

سید عنایت حسین
افسوس ہے پچھلے دنوں دیوان سیّد عنایت حسین صاحب کاانتقال ہوگیا۔ مرحوم اجمیر میں درگاہ حضرت خواجہ غریب نواز رحمۃ اﷲ علیہ کے سجّادہ نشین تھے۔ اس نسبت سے اگرچہ وہ صوفی تھے اور جمعیۃ الصوفیاء ہند کے صدر بھی تھے لیکن آج کل کے عام سجادہ نشینوں کے برخلاف شریعت کے احکام و آداب اوراتباعِ سنّت کا حتی الوسع بڑا لحاظ اور خیال رکھتے تھے۔خود بھی بڑے اچھے عالم تھے اور علماء کااحترام کرتے اوران سے تعلقات رکھتے تھے۔ درگاہِ مذکور کاسجادہ نشین ایک چھوٹی موٹی ریاست کامطلق العنان والی ہوتا ہے ۔لیکن دیوان صاحب مرحوم فقیرانہ زندگی بسر کرتے تھے اورمسلمانوں کے قومی وملی معاملات سے بڑی دلچسپی لیتے تھے ۔اخلاقی اعتبارسے بڑے خوش مزاج ،شگفتہ طبع ،متواضع اور بڑے خلیق تھے۔ ان کادروازہ ہرحاجت مند کے لیے کھلا رہتا تھا۔گفتگو بھی بڑی شیریں اور موثر ہوتی تھی۔اﷲ تعالیٰ کروٹ کروٹ جنت نصیب کرے اورمدارج ومراتب بلند فرمائے۔آمین۔ [اکتوبر ۱۹۵۹ء]

 

Prognostic Significance of Cellular Iron Metabolism in Breast Cancer

Breast carcinoma is among the most common malignancy in women. Objective: Aim of the present study was to evaluate the prognostic significance of iron expression in the biopsies of patients with breast cancer. Methods: 24 breast biopsies were studied. 19 cases were poorly differentiated, 5 cases were moderately differentiated and there was no well differentiated case. Iron, Estrogen receptor (ER), Progesterone receptor (PR), HER2 and Ki-67 immunohistochemical staining was performed for all these cases. Results: Among the 5 moderately differentiated cases, 3 (60%) were positive for iron staining and among 19 poorly differentiated cases, 11 cases (57.89%) were positive. More iron positive cases (7 out of 14) were triple positive belonging to Luminal B class. Out of 14 iron positive cases, 11 were positive for HER2, 10 for ER, 9 for PR and all positive for Ki-67. Conclusions: Iron deficiency in premenopausal and overload in post-menopausal women can contribute to the development of breast carcinoma. So, iron can be considered as a cheap and effective marker for the prognosis of breast cancer. Association between a risein iron levels and HER2 expression may providenewstrategy for breast cancer treatment.

Expression and Transformation Studies of Antifreeze Protein Gene of Carrot in Potato

The Antifreeze protein gene (1.022Kb) from local of D.carrota cultivar T29 was amplified from four weeks old seedling leave tissue genomic DNA. The PCR amplified gene was cloned in TA-Cloning vector pCR2.1. The cloned DcAFP gene was then sequenced, the DNA BLAST homology with wild carrot AFP was 96.1%. The 999bp gene of DcAFP from T29 cultivar was translated into amino acid sequence, the translated amino acid sequence when aligned with wild carrot AFP gene of amino acid sequence. The gene has 13 amino acid variability. The Asparagine amino acid residues which play an important role as an ice interacting/ice binding sites were intact as in wild species of carrot amino acid sequence, but extra three Asparagine amino acid were noted in 332 amino acid gene sequence as in the case of DcAFP (T29). The 944bp DNA sequence that code the mature peptide of DcAFP was cloned in pET15b E.coli expression vector. The IPTG induced 36kDa protein detected from the BL21 expression host transformed with expression plasmid. The 36kDa recombinant protein inclusion refolded and purified for polyclonal antibody production. Purified IgG of DcAFP was used in western blot, protein dot blot and ELISA analysis of transgenic potato lines. The 999bp full length gene was ligated in pCAMBIA-1301 without selection marker. Plant expression construct pCAMBIA1301hph - DcAFP (T29) was transformed in Agrobacterium host strain LBA4404. Agrobacterium with pCAMBIA construct was transformed in potato nodal explant. The transgenic plant first selected by PCR, DNA dot blot and GUS reporter assay. Stable integration confirmed by southern blot analysis. The DcAFP mRNA detected by Northern blotting after cold induction of transgenic potato plants. DcAFP protein detected by protein dot blot and western blotting methodologies. The sandwich ELISA was successfully done for expressed protein estimation in transgenic potato lines. The stable potato transgenic lines were tested with ILA (Ion Leakage Assay), which results are significant when compared with the control plants. It was noted that due to DcAFP protein expression in transgenic potato lines demonstrating that transgenic plants are tolerating the frost temperature.