الاصابہ فی تمییز الصحابہ میں نماز و روزے سے متعلقہ روایاتِ اَحکام کا تحقیقی جائزہ Research Review of the Traditions of Rulings on Prayer and Fasting in Al-Aṣābah Fī Tamīz al-Ṣaḥābah

The book “Al-Aṣābah Fī Tamīz al-Ṣaḥābah” is a famous book of Ibn Ḥajar ‘Asqalānī on the life and contribution of the Companions. This book is quoted as a reference to decide the status of companions. It would not be wrong to call it the encyclopedia of the companions of the Holy Prophetﷺ. Apart from its status as a reference book regarding the companions this book encompasses the Seerah of Holy Prophet in a detailed and well composed manner. The distinction of the book is not hidden from the experts of science and research. In the book and other similar books, the life of the companions was discussed and the biography of the Prophet ﷺwas also described, but another aspect that remained unexplored in these books is the virtues and commandments mentioned in it. There are also narrations about virtues. In this paper the researcher has evaluated the Hadiths related to prayer and fasting that are narrated in Al-Aṣābah Fī Tamīz al-Ṣaḥābah. The evaluation comprises of authenticity of narrators and text, narrators’ mutual association or disassociation and the final decree about the authenticity of a hadith in the light of all available evidences.

Analysis of Cytochrome Oxidase-I Gene in Tilapia Fish Species Tilapia Zillii

DNA barcoding is a technique concerned with the classification of things that is done with the help of small gene or DNA sequence with a known location on a chromosome that can be used to identify individuals or species in organisms mitochondrial DNA (mt DNA) which is helpful in recognition of meticulous species. It uses sequence variety in a 658-base pair fragment near the 5? end of the mitochondrial cytochrome c oxidase subunit I (COI) gene as a means for species recognition. DNA barcoding is a more defined and steady method in comparison with the classification based on the form and structure of the organism. It is equally useful in any stage of life cycle of fishes. The present research project was designed to recognize tilapia fish species (Tilapia zillii) of Pakistan genetically. Blood samples (n=30) were collected from Tilapia fish and genomic DNA was extracted and confirmed by 1% agarose gel. A short segment of COI gene (680bp) was amplified. PCR products were sequenced and analyzed by bioinformatics tools. Number of the haplotypes was 7. The haplotype diversity was Hd: 0.584 while nucleotide diversity was Pi: 0.00244. The mean intraspecific K2P genetic distance was 0.019. The estimated transition/transversion bias R was 1.40 that showed that this species possess very low genetic diversity. COI may supply a landmark for the classification of associated species at molecular level. As tilapia is extensively used for food and many other purposes so use of DNA barcoding technique is very helpful in discriminating it from other correlated fish species. It will also reduce the chance of mislabeling of tilapia fish species during its trading internationally as well as the species assessment at national level.