The effect of dietary vitamin E supplementation in practical diet of Labeo rohita fingerlings was experimentally evaluated in this study. The study was conducted in three feeding trials which lasted 60 days each. The basal diet was same for all feeding trials. In the first feeding trial, two adequate levels (100, 150 mg/kg) and two high levels (1000, 1500 mg/kg) of vitamin E with a control level (0 mg/kg) were fed in a completely randomized design (CRD). In the second trial, two levels of lipids (8 and 16%) and three levels of vitamin E (0, 100, 1000 mg/kg) were supplemented in a 2×3 factorial arrangement. In the third feeding trial, three levels of oil oxidation (11.7, 56.3, 111.5 meq/kg) and dietary vitamin E (0, 100, 1000 mg/kg) were used in an 3×3 factorial arrangement. Adequate levels of dietary vitamin E increased growth performance of Labeo rohita when compared to the high levels of vitamin E. Also supplementation of vitamin E linearly increased the liver and muscle α-tocopherol level. The thiobarbituric acid reactive substances (TBARS) and antioxidant enzyme activities were decreased with adequate vitamin E supplementation, which increased proportionately with increase in vitamin E concentrations. The percentages of poly unsaturated fatty acids (PUFA) were higher in vitamin E supplemented diets than control. In the second feeding trial, fish fed the 16% lipid diets showed significantly higher growth than fish fed the 8% lipid diets. Increasing the dietary supplemental levels of fish oil decreased (p<0.05) the liver α-tocopherol content, but had no effect on muscle α-tocopherol level. The TBARS and antioxidant enzyme activities increased with increasing dietary fat levels. The PUFA percentages increased significantly with increased levels of dietary fish oil. In third feeding trial, growth of fish fed fresh fish oil and low oil oxidation level were significantly higher (p<0.05) than those of fish fed highly oxidized oil. Among highly oxidized oil (111.5 meq/kg) containing treatments, fish feeding on 1000 mg/kg vitamin E showed better growth performance. Oil oxidation caused a significant reduction in the concentrations of α-tocopherol in fish liver and muscles. Ingestion of oxidized oil resulted in higher TBARS levels, antioxidant enzyme activities and muscular PUFA, in particular, DHA while dietary vitamin E supplementation abrogated these effects. In conclusion, vitamin E supplementation reduced the lipid peroxidation, enhanced the growth performance and improved the meat quality of L. rohita fingerlings irrespective of the level and quality of fish oil; however, the required level of vitamin varied depending upon dietary level of fish oil and level of oil oxidation.
The teachings of all religions are based on peace but the Islamic principles of peace surpass others in their effectiveness. For the attainment of peace and harmony in this world, it is imperative to respect all the religions. The Prophet Muḥammad (ﷺ) was indeed a peacemaker and a mercy to all the mankind. The author of this paper feels that it is also very important to study the history of Prophet Muḥammad (ﷺ) prior to his prophethood, because, those a as image and reputation (صلى الله عليه وسلم) his shaped that years the were peacemaker in the eyes of the people of Makkah. His early years of virtue soon followed by a lifetime of nobleness and greatness. The incident of the placing the Black Stone, for example, is a confirmation to the said fact. It is one of the first examples in the life. Goodwill nurturing and conflicts mitigating of (صلى الله عليه وسلم) prophet the of or himself by stone the placed have could (صلى الله عليه وسلم) Prophet Holy The asked anyone of the elders of his nation to do it, but being a peacemaker, he saw that, thatwas going to be a model to mitigate conflicts and nurture goodwill among the leaders of the tribes., first the of milestone the laid (صلى الله عليه وسلم) Muhammad Prophet The the just and the civilized human society. A commitment to peace was a way of his life. This is the quality that ought to become the cornerstone of the policy and the personality of a sound Muslim leader.
HCV has been on the top of virus-induced liver diseases in many parts of the world and has gained endemic proportions in our population. Frequency of HCV in Pakistan is significantly higher (4.7%) when compared to the populations of same ethnicity. The hepatitis C virus (HCV) is a small enveloped, single-stranded RNA virus. It is a member of the Hepacivirus genus in the family Flaviviridae. The RNA encodes a large polypeptide of about 3,000 amino acids in a single continuous open reading frame (ORF) which is flanked at the 5'' and 3'' ends by non-translated regions (5'' UTR). Viral load suppression reduces risk of hepatitis C liver morbidity and mortality and prevents progression to cirrhosis, hepatocellular carcinoma (HCC), and decompensated liver disease requiring liver transplantation. Patient race/ethnicity and HCV genotypes also affected the risk of future liver events and death. Multivariate analyses examining socio-demographic and clinical characteristics found that race was the only variable significantly associated with the difference in response rates. So we designed a study to find that how does our local population respond to Hep C treatment regimens and which treatment regimen is effective and safe. Moreover, we also wanted to know that either viral load was correlated to treatment outcome or not. We also planned to do the Pharmacoeconomic analysis of treat regimens. In our study we included adult male / female patients who were seropositive for HCV RNA were tested with real time PCR after an informed written consent. Patients with chronic liver disease, decompensated cirrhosis, anemia (hemoglobin concentration, less than 12 g per deciliter in women and less than 13 g per deciliter in men), psychiatric conditions, seizure disorders, cardiovascular disease, poorly controlled diabetes mellitus, or autoimmune diseases were excluded from the study. Initially 104 patients were evaluated for genotypes and found that 90% of the cases in our local population were infected with HCV 3a genotype. Based on specific prevalence it was decided to compare two treatment regimens (Peg INF+RV & INF+RV) only in patients infected with HCV 3a genotype. We evaluated these treatment regimens for the efficacy and safety both. The required data was recorded on structured data collection form. Their Virologic response was measured at week 0, week 4, week 12, week 24 and week 48 to evaluate treatment efficacy. The initial viral load was also compared with the final out come of the therapy. After the end of the therapy these patients were followed for sustained response. LFTs, RFTs and hematologic parameters were measured on regular intervals to evaluate drug safety. We also did pharmacoeconomic analysis of both treatment regimens being used in our local population to treat Hepatitis C virus infected patients. Our study concluded that though INF+RV treatment regimen was cheaper but Peg INF+RV treatment regimen was more affective in 3a genotype. As far as treatment safety was concerned it was comparable in both regimens. The Virologic response can be used to modify duration of therapy. Moreover, fatty liver can be used as a predictor to assess the final out come of the treatment.