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Home > Effect of Polyphenol from Apple Peel Extract on the Survival of Probiotics in Fermented Dairy Products

Effect of Polyphenol from Apple Peel Extract on the Survival of Probiotics in Fermented Dairy Products

Thesis Info

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Author

Ishtiaque Ahmad

Program

PhD

Institute

University of Veterinary and Animal Sciences

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2020

Thesis Completion Status

Completed

Subject

Technology Dairy

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12326/1/Ishtiaque%20Ahmad%20dairy%20tech%202020%20uvas%20lhr%20prr.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727742356

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Three experiments were conducted to inspect the effect of apple peel polyphenol extract on the survival of probiotics, physicochemical and antioxidant properties in three different fermented dairy products (Acidophiuls-bifidus milk, probiotic yoghurt and probiotic ice cream). In Experiment No.1, quality characteristics of apple peel polyphenol extract of five local cultivars (Red delicious, Golden delicious, Royal gala, Amri, and Gacha) were evaluated on the basis of total flavonoid contents, total phenolic contents and antioxidant activity. The objective of this study was to incorporate the best apple peel polyphenol extract in acidophilus-bifidus milk to develop a probiotic dairy product. Effect of the added apple peel polyphenol extract with various concentration (0-5%) on microbiological characteristics, physicochemical and sensory attributes of acidophilus-bifidus milk were evaluated. The viability of Lactobacillus acidophilus and Bifidobacterium lactis was assessed in the milk stored at 4°C for two weeks. The highest flavonoid contents, phenolic contents and antioxidant activities were found in red delicious and golden delicious varieties. The results revealed that apple peel polyphenol extract promote the viability of probiotics. The viability of probiotics was significantly (p<0.05) high in milk fortified with 5% apple peel polyphenol extract as compared to other treatments (0-4%) during two weeks. The viable counts of probiotics in acidophilus-bifidus milk were identified within the recommended levels (>106 log CFU/ml). The pH and viscosity were slightly decreased during storage of milk supplemented with apple peel polyphenol extract. The acidophilus-bifidus milk fortified with 5% apple peel polyphenol extract had the best sensory score. Apple peels are a cheap source of polyphenols for increasing probiotic functionality in milk products. Our findings show that apple peel polyphenol extract can be suitable substrate for the viability of probiotics in acidophilus-bifidus milk. In Experiment No. 2, the effect of apple peel polyphenol extract on the microbiological and physicochemical properties of probiotic yoghurt throughout storage was studied in this research activity. Yoghurt was fortified with various concentration of apple peel polyphenol extract (0-5%). The increase in acidity, water holding capacity and viscosity were observed while no significant effects were witnessed on milk fat and total solid contents of probiotic yoghurt with the addition of apple peel polyphenol extract. The total phenolic contents of probiotic yoghurt fortified with apple peel polyphenol extract increased significantly as 0.59, 0.71, 0.97, 1.18, 1.35 in AE1, AE2, AE3, AE4 and AE5 respectively. The results showed that apple peel polyphenol extract promoted the sustainability of probiotics and improved the antioxidant activity of probiotic yoghurt. The low viability loss of probiotics was observed in yoghurt with the fortification of apple peel extract significantly (p<0.05) throughout storage. The viability of probiotics during storage was high in yoghurt contained 5% apple peel polyphenol extract. The high viability loss of probiotics was significantly (p<0.05) attained in control yoghurt. In Experiment No. 3, evaluated the effect of polyphenol from apple peel extract on probiotic viability; physicochemical, and textural characteristics; and sensory properties of probiotic ice cream stored at -20°C for three months. All ice cream had viable count of Lactobacillus acidophilus and Bifidobacterium lactis of ≥8 log CFU/g and >7 log CFU/g respectively during storage except the control sample until day 90 of storage. The highest viability of probiotics was obtained in the sample fortified with 5% apple peel polyphenol extract, whereas it showed the highest hardness after day 90 of storage. The presence of apple peel polyphenol extract increased the titratable acidity, reduced the melting rate of the ice cream, and enhanced the overrun. The hardness of experimental samples improved with the fortification of apple peel polyphenol extract. The addition of apple peel polyphenol extracts significantly increased melting resistance; however, panelists generally like these samples (AE4 and AE5) in terms of taste, flavor, color and texture. The outcomes of this research presented the potential for the processing of synbiotic fermented milk product including a probiotic strain (Lactobacillus acidophilus and Bifidobacterium lactis) and apple peel polyphenol extract as prebiotic ingredient. It was concluded that the survival of probiotics and antioxidant activity were enhanced by the presence of apple peel polyphenol extract. It was concluded that the probiotic milk products fortified with apple peel polyphenol extract can act as natural source of highquality antioxidant and bioactive compounds. Fortification of apple peel polyphenol extract at 5% in probiotic milk products was more favorable for the survival of Lactobacillus acidophilus and Bifidobacterium lactis, whereas 4% positively affected more sensory attributes of yoghurt. In the low revenue countries like Pakistan, the waste of apple like peels could be an economical source of polyphenols for increasing probiotic functionality in fermented milk products. These observations indicate the feasibility of apple peel polyphenol extract in the development of high nutritious and value-added probiotic food products.
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مولانا سید محمد میاں

مولانا سید محمد میاں
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کوئٹہ کے مراکز افتاء اور ان کی کارکردگی

In Sharia (Islamic Law), the interpretation of any law to solve an issue or problem with a logical verdict, of an individual in society or state, is called Fatwahs. Fatwahs are issued from the religious institutions, called “Marakiz e Ifta”. While the “Muftees” are considered the sole authority to disperse and verdict for all issues in an Islamic society or state. Without the interpretation of Muftees, the solution for all issues in an Islamic society is impossible. No individual can deny the importance of “Fiqh” and “Fatwahs” in Islamic society. These religious practices and services of Muftees with Fiqah, have been existing since its origin. In the same way, such services and practices are present in Quetta city as in the other regions of Pakistan. In this study, the researcher has analyzed the following aspects such as: Muftees, their religious Fatawahs and Maddaris, their establishment, services and practices regarding these Fatawahs and its characteristics, the merit of an issue for a verdict and the importance of these Fatawahs on official level. The objectives of this study are the analysis of the services of Muftees and Maddaris of Quetta. That the Muftees and Maddaris have practiced and served the general public as in the other regions of Pakistan. The research site for this study was Quetta city. The research toll was interview technique while the respondents or participants for this study were religious Schalors. Data was collected and analyzed by the researcher. This study comes within the qualitative research paradigm.  

Creatine Monohydrate As a Neuroprotective Agent in Female Albino Mouse Following Neonatal Hapoxia Ischemia Encephalopathy

Female albino mice, on postnatal day 10, were subjected to left carotid artery ligation followed by 8% hypoxia for 25 minutes. During short term experiments three sensorimotor reflexes (Righting, Cliff Aversion and Negative geotaxis) were determined along with brain infarcts to demonstrate the effect of hypoxic-ischemic insult. During long term experiments, mice with and without hypoxic ischemic insult (HI and NO HI) were divided in to three treatments on the basis of diet supplementation (Normal rodent diet, 1% and 3% creatine supplemented diet) for 10 week following weaning on postnatal day 20. A battery of neurological tests was used to asses long term neurofunction (Rota rod, open field and Morris water maze) along with infarct measurement and determination of interleukin-6 and 18 in serum. During short term experiments mice subjected to hypoxia ischemia on postnatal day 10 exhibited poor sensorimotor reflexes 1 and 24 hour after brain injury. During long term experiment, it was observed that overall creatine supplementation in both HI and NO HI group improved neuromuscular performance but the affect was more pronounced in 3% creatine supplemented treatment. In open field, creatine supplementation enhanced locomotory and exploratory behaviour significantly in HI treated mice. During morris water maze it was observed that creatine supplementation improved spatial memory and enhanced swimming speed in both HI and NO HI groups. A significant affect of creatine supplementation in reducing infarct size was also observed. It was noticed that creatine supplementation helped in reducing IL-6 concentrations while no significant effect was observed on IL-18 concentrations in serum of female albino mice during long term experiment following hypoxic ischemic insult. Overall female albino mice supplemented with creatine monohydrate during neurological studies demonstrated better learning abilities and neuromuscular coordination and the affect was more pronounced in HI treated mice. It was also observed that the mice supplemented with 3% creatine diet performed better than mice on 1% creatine diet during series of neurological test batteries in both HI and NO HI group.