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Protein Subcellular Classification Using Machine Learning Approaches

Thesis Info

Access Option

External Link

Author

Tahir, Muhammad

Program

PhD

Institute

Pakistan Institute of Engineering and Applied Sciences

City

Islamabad

Province

Islamabad

Country

Pakistan

Thesis Completing Year

2014

Thesis Completion Status

Completed

Subject

Computer Science

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/2055/1/2352S.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727816357

Similar


Subcellular localization of proteins is one of the most significant characteristics of living cells that may reveal plentiful information regarding the working of a cell. Subcellular localization property of proteins plays a key role in understanding numerous functions of proteins. The proteins, located in their respective compartments or localizations, are in- volved in their relevant cellular processes, which may include cell apoptosis, asymmetric cell division, cell cycle regulation, and spermatic morphogenesis. In fact, cells may not perform their regular operations well in case proteins are not found in their proper subcellular lo- cations. Improper localization of proteins may lead to primary human liver tumors, breast cancer, and Bartter syndrome. Protein sequencing has observed rapid expansion due to the advancement in genomic sequencing technologies. This led the research community to recognize the functionalities of different proteins. In this connection, microscopy imaging is providing protein images well in time with low cost compared to protein sequencing. However, automated systems are required for fast and reliable classification of these protein images. Comprehensive analysis of fluorescence microscopy images is required in order to develop efficient automated systems for accurate localization of various proteins. For this purpose, representation of microscopy images with discriminative numerical descriptors has always been a challenge. This thesis focuses on the identification of discriminative feature extraction strategies effective for protein subcellular localization, the recognition capability of the prediction sys- tems, and the reduction of classifier bias towards the majority class due to the imbalance present in data. The contributions of this thesis include (1) Analysis of different spatial and transform domain features, (2) Development of a novel idea for GLCM construction in DWT domain, (3) Analysis of SMOTE oversampling in the feature space, (4) Analysis of GLCM in the spatial domain for capturing discriminative information from fluorescence microscopy protein images along different orientations, (5) Exploitation of Texton images for their capability of extracting discriminative information along different orientations from fluorescence microscopy protein images, (6) Development of the web based prediction sys- tems that can be accessed freely by the academicians and researchers. Extensive simulations are performed in order to assess the efficiency of the proposed pre- dictions systems in discriminating different subcellular structures from various datasets.
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14. Ibrahim/Prophet Abraham

I/We begin by the Blessed Name of Allah

The Immensely Merciful to all, The Infinitely Compassionate to everyone.

14:01
Alif. Lam. Ra’.
This is the Divine Book - The Qur’an - WE have sent it down upon you - O The Prophet-
so that you may lead people out of the darkness into the light by the Permission of their Rabb - The Lord,
onto the Path of the Almighty, the All-Praised ….

14:02
…. Allah, to WHOM belongs whatever is within the celestial realm and whatever is within the terrestrial world.
Are you going to reject it?
Do not forget that for those who reject it, there is a doom of a terrible punishment.

14:03
Those who love the life of this world more than the life of the Hereafter,
and obstruct the Path of Allah, seeking to make it appear crooked and faulty-
they are far straying from the truth.

14:04
And WE never assigned any Messenger to a community with a message except in the language and idiom of his people, so that he could make the Message clear to them.
But despite this Allah lets go astray whoever wills to go astray, and HE guides whoever wills to be guided.
And HE is the Almighty, the All-Wise.

14:05
And, indeed, WE assigned Moses with OUR Miraculous Signs to Pharaoh, instructing:
‘Lead your people – Descendants of Jacob - out of the darkness into the light, and
remind them of Allah’s Days.
Truly in that are messages for every steadfast in adversity and grateful’ person.

14:06
And recall when Moses said to his people:
‘Remember Allah’s favors...

PHYSICAL THERAPY TREATMENT IN POSTNATAL WOMEN

Background: The postnatal period starts at the time when a mother gives birth to a baby; all changes that occur during pregnancy come back to a normal state like hormonal levels, size of the uterus, and weight. According to WHO this is the most ignorant period for postnatal women and their children by the society which is a bitter reality that’s why death is common in many mothers and children during the postnatal period. In Physical therapy, Gynecological rehabilitation is an important part of treatment and also a topic of consideration for referrals to doctors in the postnatal period to improve the issues related to postnatal women. Objective: To enhance the knowledge regarding the role of Physical therapy in the postnatal period of women. Search Strategy: This is a review article with extracts from various search engines like PubMed, CDC, MP, MCHI, Google scholar, Sci-Hub, etc. It is used to increase the knowledge regarding various treatment options in physical therapy that are helpful for postnatal women without causing side effects. Conclusion: Physical therapy consists of many interventions that can be beneficial, and it improves the quality of life of postnatal women.

Assessment of Patient Satisfaction With Primary Health Care at the Family Medicine Centre of Aga Khan University Hospital, Nairobi

Background: Patient satisfaction is one of the key indicators by which the quality of healthcare service is evaluated. The World Conference of Family Doctors African Chapter's (WONCA-Africa) encourages Family Medicine practices to regularly assess their patients' satisfaction as one of the criteria for benchmarking and auditing the quality of primary care in Africa. The Kenyan government, in a quest to improve primary care provision, has formulated policy to make family physicians essential players in coordinating and enhancing standards of primary care. Objective: This study aimed to assess patient satisfaction with the quality of primary health care services provided by family physicians at the Family Medicine Clinic Aga Khan University Hospital Nairobi. Methods: A cross-sectional, descriptive, quantitative study using a modified version of the post-consultation General Practice Assessment Questionnaire (GPAQ-R) was conducted. A total of 317 patients were selected through systematic random sampling. Level of satisfaction was calculated by averaging summing the total scores of different domains in the questionnaire. The cut off for the level of satisfaction was 82 points out of a possible 128 points. Exploratory Factor analysis derived factors affecting overall satisfaction. The correlation of the independent variables with overall satisfaction was analysed using the Chi-Square test. Results: Of the 310 study respondents, 93% were satisfied with the care provided. The overall mean patient satisfaction score (standard deviation) was 103.14 (14.2). Factors that mostly contributed to overall patient satisfaction were effective communication (variance 31 %) and timeliness of care (variance 8 %). Patients with long-standing conditions were more satisfied than those with acute conditions. (99.01 % v 90 %, p=0.006). Conclusion and recommendation: Patients were highly satisfied with the care provided at the Family Medicine Clinic. Communication and timeliness of care contributed to overall satisfaction. Patients' with chronic conditions were found to be satisfied with the care provided. Additional research that incorporates a care provider's perspective may be required to better elucidate enablement and empathy as factors that affect patient satisfaction. Furthermore, we recommend a study that addresses financial accessibility, infrastructure and clinical competencies of care providers in the assessment.