خدمت خلق
نحمدہ ونصلی علی رسولہ الکریم امّا بعد فاعوذ بااللہ من الشیطن الرجیم
بسم اللہ الرحمن الرحیم
معزز اساتذہ کرام اور میرے ہم مکتب شاہینو!
آج مجھے جس موضوع پر اظہار خیال کرناہے وہ ہے:’’خدمت خلق‘‘
صاحب صدر!
خدمت خلق ایک ایسا جذبہ ہے جس سے معاشرہ میں اخوت، ہمدردی اور بھائی چارہ کار جحان پروان چڑھتا ہے، مرجھائے ہوئے چہرے کھل اُٹھتے ہیں ، افسردہ دلوں میں خوشی و مسرت کی لہر دوڑ جاتی ہے۔
صدرِذی وقار!
خدمتِ خلق کا جذبہ رکھنے والا شخص کبھی حالات کے تھپیڑوں سے گھبراتا نہیں، جملہ امور کی انجام دہی سے سر خرو ہوتا ہے، پژمردہ دلوں کی ہمدردیاں اس کے ساتھ ہوتی ہیں اور یوں وہ ہر میدان میں کامیابی و کامرانی کے گھوڑے دوڑاتا ہوا آگے بڑھتا جا تا ہے۔
جنابِ صدر!
خدمتِ خلق حقوق العباد میں سے ہے، اور حقوق العباد کی ادائیگی اسلامی عبادات کا ایک اہم جزو ہے، حقوق اللہ کی معافی کا امکان بہرحال موجود ہے لیکن حقوق العباد کی ادائیگی کے بارے میں باز پرس ہوگی۔
صاحبِ صدر!
خدمتِ خلق کے لیے انفاق فی سبیل اللہ کی عظیم صفت سے متصف ہونا انتہائی ناگزیر ہے، مال خرچ کرنے سے عوام النّاس کے قلوب میں مخیر حضرات کی عزت میں اضافہ ہوتا ہے اور یوں محبت کی فضاء پروان چڑھتی رہتی ہے۔
جنابِ صدر!
خدمتِ خلق کے مختلف انداز ہوتے ہیں ، والدین کی خدمت، اساتذہ کی خدمت ، ضعیف حضرات کی خدمت، کمزوروں اور ناداروں کی خدمت، بے کسوں اور کسمپرسوں کی خدمت، اصدقا و اقربا کی خدمت، ہمسائیوں اور عزیزوں کی خدمت، یہ سب خدمت خلق سے ہی ہے۔
لیکن جنابِ صدر!
وہ لوگ قسمت کے سکندر ہیں ، مقدر کے دھنی ہیں جوطلباء کی خدمت کرتے ہیں، تو ان کے علمی میدان میں...
Among different ways of exegeting based on narrative method (Tafseer bil-Ma’thoor), the most reliable form is to interpret the Quran by Quran itself. There are numerous ways in which Quran elaborates its meaning one of which is the use of Qira’aat (i.e. Dialectical method). The use of different variations of reciting Quranic words elaborates its meaning. An important point to note here is that the difference in Qira’aat corroborates the diversity in the meaning and their comprehensiveness not their inconformity. Qira’aat are categorized by the scholars in two categories: There are those that are narrated and transmitted by multiplicity (Tawaatur) while others do not fulfill such criteria and are therefore denoted by the term (Shaazzah). This papers seeks to substantiate the method of interpreting the Quran by both forms of Qiraa’aat and concludes that both of these were actually revealed by Allah and are both reliable in terms of exegeting the Quranic text
Among abiotic stresses, heat stress has the most devastating impact on plant growth. In the present study, heat shock transcription factor HsfA1d was isolated from Arabidopsis thaliana and cloned into an in-house constructed gateway compatible cloning vector pUC57GW-CmRccdB through infusion cloning. Entry clone was confirmed through restriction digestion and sequencing analysis. For subcellular localization, HsfA1d was cloned in-frame with yellow florescent protein (YFP) in pGWB442 through LR clonase reaction resulted in YFP-HsfA1d chimeric gene construct. Homodimerization of HsfA1d was studied using Bimolecular Florescence complementation (BIFC) assay. For BIFC, HsfA1d was cloned in-frame with sequences that codes for N and C termini of yellow florescent protein, into 2 in-house constructed vectors pGSA002-nYFPn and pGSA002-nYFPc respectively. After confirmation through restriction enzyme digestion, the newly constructed vectors were transformed into Agrobacterium tumefaciens GV3101 for transient expression in tobacco (Nicotiana benthamiana) through syringe-infiltration. Subcellular localization of YFP-HsfA1d in DAPI-stained cells was evaluated using confocal microscopy. For plant transformation, HsfA1d was cloned into 2 plant expression vectors pGWB402Ω and pGWB442 through LR clonase reaction. After confirmation through RFLP analysis, the vectors were transformed into Agrobacterium GV3101 for tobacco leaf disc infection. Putative transgenic plants were selected and regenerated on appropriate selection media using different growth regulators. After PCR and confocal-based confirmation, transgenic plants were evaluated for their response to heat stress. HsfA1d, HSP70 and HSP90 exhibited 4.8, 2.8 and 2 folds increase respectively in their expression under heat stress compare to room temperature. HsfA1d was found to significantly enhance the expression of downstream gene HSP70 while no such effect on the expression of HSP90 was recorded. The plants transformed with HsfA1d were found to retain more water and accumulate more proline under heat stress. The transgenic plants exhibited efficient protective system, causing less 8 electrolyte leakage and less chlorophyll damage under heat stress. It is concluded that HsfA1d plays a vital role in thermotolerance enhancement and hence recommended for increasing plant thermotolerance.