کوئی طبیب ہی ایسا نہ چارہ گر کوئی
کرے ہمارا مداوائے چشمِ تر کوئی
مرے وجود سے اُٹھنے لگی ہیں خوشبوئیں
کہ ایسے مجھ میں سمایا ہے عشوہ گر کوئی
میں اُس کو حالِ دلِ زار کیسے بتلاتا
مجھے ہوا نہ میسر پیام بر کوئی
مرے حضور یہ زندہ دلوں کی بستی ہے
نہ بے ضمیر یہاں پر نہ کم نظر کوئی
امیر شہر! یہ حالت تری رعایا کی
ہے پا برہنہ کوئی اور برہنہ سر کوئی
خدا کے واسطے ناصح ہمیں اجازت دے
کہ انتظار میں اپنے ہے بام پر کوئی
ہے میرے حال سے دنیا تو باخبر تائبؔ
ہے میرے حال سے کیوں اتنا بے خبر کوئی
This paper deals with Shariah and Tariqat. The decrees of Shariah have two kinds. One is related to the body (which is visible) , while the other one is about the soul (in visible) . Shariah is a combination of visible and un visible decrees. Tariqat is not a separate entity, it is cm integral part of Shariah. The people who consider Tariqat as a separate entity-, as a matter offact they are misguided persons. Tariqat is nothing but a subservient to Shariah. For the purification ofsoul and to make one self abiding by the laws of Shariah, the Sufia have proposed some specific practices and activities for their followers. They asked their followers to strictly adhere to these practices. Not only for the followers hut also for the spiritual guides; it is incumbent on them to follow the rules ofShariah whether these are related to body or to the soul. Irrespective of the position of the spiritual guide, he should also abide by the laws ofShariah. His Sufipractices. supernatural events or miracles have no value and no acceptance to the Almighty - Allah. Tariqat must be under the rules of Shariah, other wise it will not be accep
The lignin degrading enzymes system of WRF has incredible catalytic property for oxidative bio-remediation of a lot of toxic contaminants and numerous other industrial applications. Immobilization facilitates the re-use of enzymes and making them industrially applicable and eco-nomical bio-catalysts. Schizophyllum commune IBL-06 was used for the production of lignin peroxidase (LiP) in pre-optimized solid state fermentation medium of corn stover. A high yield of LiP (1347.3 U/mL) was observed in crude extract. LiP was purified (5.65 folds) by a 4 steps protocol containing ammonium sulphate fractionation, dialysis, ion-exchange (DEAE-cellulose) and size exclusion chromatography. The 38 kDa single polypeptide S. commune IBL-06 LiP migrating as a single clear band on both native and SDS-PAGE gels. The purified enzyme was then immobilized on different support matrics such as chitosan, alginate, nylon membrane, PVA, agarose and PA gels with high immobilization efficiency and stability. Scanning electron microscopy (SEM) was performed for the verification of LiP attachment on support matrices. The maximum dye-decolorization (95.45%) potential was observed with chitosan and ca-alginate immobilized enzyme at 30°C without hemolytic toxicity. The chitosan beads-LiP retained approximately more than 70% activity after 3 frequent runs that gradually reduced to 35 % after 7th cycle of reusability. The immobilized LiP was found to show superior dye removal properties as compared to free LiP. Higher thermo stability, lower Km and high Vmax features of chitosan beads immobilizedLiP suggested its suitibility for variousbiotechnological and industrial applictaions.