تڑپ رہا ہوں بُلاوا مگر نہیں آتا
مدینے جاؤں پہ اِذنِ سفر نہیں آتا
تری ثنا سے نہ جب تک حروف تر ہوں مرے
سُخن کی شاخ پہ تب تک ثمر نہیں آتا
دلوں کا حال بدلتا ہے میرا ربّ لیکن
بغیر تیری دُعا کے عمرؓ نہیں آتا
کرم کہ ذات تری افصح العرب ٹھہری
کہ نعت لکھنے کا مجھ کو ہُنر نہیں آتا
درود گر نہ پڑھیں اِس کے اوّل و آخر
دُعائے نیم شبی میں اثر نہیں آتا
There are two major parts of Arabic literature i.e. Prose and poetry. Arabic poetry has a great value among the critics and the literati. A series of seven poems known as Muʽallaqāt Sabʽa or ʽAšhara have a great deal in the Arabic poetry. These are the collection of seven or ten long poems that are considered as the excellent work of the pre-Islamic era known as Jāhilīya ages. These poems had been presented in the annual fair of Okaz on the occasion of pilgrimage and awarded to be the top class creative works. After that judgment, golden genres were written with silk and recited judgment, as well as taught consecutively up to date. The ancient Arabic literature is full of such like prized poems but the valuable position met to this compilation is unprecedented. Since the poets of these master pieces are among the most famous figures of the 6th century taken together, these poems provide a good picture of Budouin life besides its connotation of rhetorical semantics figurative devices are tricky during its studies. Metaphor, trope, allusive ironies, metonymies and many more colloquial figures of speech are to be observed in this renowned collection. This article deals with the rhetorical study of Muʽallaqa of Imru’ al-Qais, by identifying the magnificence of assimilation and critism on his poem, alond with the analysis and explanation of its objectives.
Enzyme immobilization utilizing distinctive methodologies and different supports appears to be the most effective possibility for developing industrially compatible enzymes. Immobilization has been discovered as a capable device for enhancing all catalyst properties, specific activity, thermo stability and decreasing inhibition. Laccase with improved catalytic features is a potentially attractive biocatalyst with many possible applications including bioremediation, biomass delignification for (590706) 4.58 Visual comparison of decolorization of dyes with free and immobilized laccase with chitosan bead (a) Sandal-fix Red C4BLN (b) Sandal-fix Golden yellow CRL (300410) 138 4.59 Visual comparison of dye degradation (A) control (B) degradation with free Laccase (C) degradation with laccase immobilized with PVA beads (D) degradation with laccase immobilized with Ca-Alginate beads 140 4.60 Repetitive use of immobilizedlaccase for dye decolorization 142 4.61 Storage stability of free and immobilized Laccase at 16oC 144 ethanol production, chemical synthesis, bio pulping and biosensor development, denim stone washing, cosmetics, dyes degradation, textile finishing and wine stabilization. In this study, four copper containing extracellular laccase was purified from culture filtrate of an indigenous WRF strain Trametes Versicolor IBL-04, grown in solid state fermentation (SSF) under pre optimized condition using corncobs as a substrate. The enzyme was purified (1.00-4.03 fold) to apparent electrophoretic homogeneity through ammonium sulphate precipitation, dialysis, DEAE-cellulose ion exchange and Sephadex G-100 gel exclusion chromatography. The purified enzyme elucidated a monomeric single band on Native and SDS polyacrylamide gel electrophoresis (SDS-PAGE) with an apparent molecular weight of 66 kDa. With an aim to improve its functionalities, the purified laccase was effectively immobilized on nine supporting matrices using diversified immobilization techniques and the relative activity at optimum temperature and pH and thermal stability of free and immobilized laccase were studied for comparative analysis. Scanning electron microscopy (SEM) and Transmission electron microscopy (TEM) were performed to characterize enzyme immobilization. Immobilized laccase was successfully used for the decolorization of six different synthetic (textile) dyes with more than 98% removal rate. The resulted modified laccase enzyme was reusable for up to many cycles in dye color removal and also check the storage stability and thermostability of immobilized laccase. All immobilized parameters showed that immobilized laccase is better for industrial use in comparison to free laccase. Finally results of this study suggested that immobilized laccase catalytic system can be efficiently exploited for dye degradation due to its sustainability, durability and reusability over its soluble counterpart. Keywords: Trametes Versicolor IBL-04, Laccase, purification, characterization, immobilization, dye decolorization