Introduction
In the Indo-Pak sub-continent, Sindh is the first of the zones where the advent of Islām was the earliest. Historically, the scholars here did a great work of intellect and research. In the year 270 A. H/883A. D, due to internal disturbance, so many small states came into being. [1]
Out of these, the state of Manṣūrah was taken over by the Ḥubārī tribe. Muslim ruler ‘Abdallāh bin ‘Umar Ḥubārī of Manṣūrah had sent a scholar to Hindū Rājāh of Alvar on his request who presented the translation of the Qur’ān in his court. This incident narrated by the famous historian Buzurg Bin Shehr Yār has also been endorsed by renowned historians of the sub-continent. [2]But nothing with certainty can be said about the symmetrical order and the dictation of this translation, however, it is certain that the honor of translating the Qur’ān the first of all lies with Sindh. [3]
In the era of Arghaun and Tarkhān, Makhdūm Nūḥ Hālāe’ (d:998 A. H/1589A. D) properly translated the Qur’ān in Persian which has become available in secured form despite the varying times. [4]
This translation, with the efforts and research of ‘Allāma Muṣṭafā Qāsmī (d:1424A. H/2003A. D) has been published by Sindh Literary Board Ḥyderābād. This translation was compiled even earlier than the translation of famous researcher Shāh Walī Allāh(d:1176A. H/1762A. D). In this regard, we are justified to opine that in the sub-continent the honor of the first translation in the Persian language also goes to Sindh. In that period, a movement among the Sindhī Scholars emerged that the Islamic literature and art be documented in the mother tongue Sindhī so that a...
It is an established fact that the mainthrust driving force of all Islamic disciplines is the personality of Prophet Muhammad (PBUH) who appeared in the Arab Peninsula, spreading the rays of divine wisdom throughout the globe. He emerged and prevailed the Prophetic message in such a vivid style that the whole humanity confessed his lasting impressions. The Indian sub-continent has also been one of the impressed territories despite of having no direct social, cultural or religious relationships, yet some approaches from Indian sub-continental Rajas and presentations of gifts to the Prophet (PBUH) have been recorded in the history. Historians have expressed the reality that before Muhammad bin Qasim, a numbers of companions of Prophet reached India and played a pivotal role in making the suitable ground for embracing Islam. Hence, the Arab epistemological tradition engraved in the core of Indian hearts which consequently, reappeared and emerged in the classical exegetical literature of sub-continental scholars, such as Shah Waliullah and his off shoots both in India and Pakistan. This paper has been specifically articulated to explore the Arab impression on exegetical writings in the sub-continent
Infectious bronchitis virus (IBV) is incriminated in a variety of clinical conditions in poultry. IBV has a potential to mutate under field conditions and due to this multiple serotypes and variants of the IB virus circulates in commercial and backyard poultry. In the present study seroprevalence levels of different known serotypes of IBV such as, Mass-41, 4/91, D274, D1466, IT-02, D388 & D8880 were first determined in non IB-vaccinated poultry from different provinces of Pakistan during 2012 to 2015. The data showed high seroprevalence of multiple serotypes of IBV indicating high level of diversity in the circulating serotypes/variants of IBV in this country. In addition to this, a post-IBV vaccination base line was developed by sero-monitoring among healthy poultry in response to IBV vaccines, providing a permanent reference for future monitoring of post-vaccination antibody titers For further IBV investigations, different diagnostic techniques to be used in this study were optimized in this study. Moreover, studies regarding the determination of tissue tropism of one of the new IBV isolates along with evaluation of its co-infection potential with Avian Influenza virus H9N2 and ORT were also carried out. For the detection and typing of locally circulating Pak-IBV isolates following techniques were first optimized including, haemagglutination (HA), haemagglutination inhibition (HI), Indirect-Enzyme linked immunosorbent assay (ELISA), restriction fragment length polymorphism (RFLP), reverse transcriptase polymerase chain reaction (RT-PCR), real time reverse transcriptase polymerase chain reaction (QRT-PCR), virus neutralization (VN). Using these techniques, a total of 3187 clinical samples were processed for IBV detection, out of this 871 were IBV positive. Moreover, subtype detection revealed that 45.2% was Mass-41, 51.3% was IBV serotype 793-B and 3.4% were variants or un-identified. Furthermore, 871 RT-PCR positive samples were propagated upon in-ovo inoculation in specific pathogen free (SPF) embryonated chicken eggs. Out of this 55 IBV isolates were subjected to RFLP analysis that grouped the isolates into three segments, first was designated as 4/91 like group, second was designated as Mass-41 like group and the third was designated as IBV variant. The sequence and phylogenetic analysis of three IBV isolates from each RFLP group revealed that the first isolate, Pak IBV-786, shared sequence homology of the range of 25 99.1%-99.5% with 4/91 like strains from China, India, Russia, Morocco, Japan and Iran or GI-13 IBV lineage. The second isolate, Pak IBV-1113 shared 98% sequence of its sequence with IBV vaccine strains of Ma5 and M41 from Brazil, India, USA, Egypt, China, Iran, Thailand and Poland. The third and new Pak isolate, IBV-973, shared 91-93% of its sequence with the Indian strains of IBV earlier reported in India only, of GI-24 lineage. This strain of IBV variant has been first time reported from commercial poultry in Pakistan and its subsequent molecular characterization revealed that this virus is in fact a new serotype of IBV earlier only reported from India. Pathogenesis and co-infection studies on the isolate Pak-973 further highlighted biological characteristics of the new Pak-variant, which led us to believe that this variant may be contributing significantly towards the development of super complex of Respiratory Tract Infection, with or without the involvement of AIV H9N2. The sequence and phylogenetic analysis of different Pak IBV isolates reflected here that the genome of IBV is under a continuous process of evolution, due to point mutations, selective pressure (vaccine) and recombination events. So as like other RNA viruses, the IBV control is most likely to succeed upon using serotype specific vaccines (Homologous vaccines), as carried out elsewhere. It would, therefore be highly appropriate to recommend the incorporation of strain Pak-973 in commercial poultry vaccines being used in this country. INDEX WORDS: Infectious bronchitis virus, Pak-variants, Spike glycoprotein, RFLP, Real time RT-PCR, Co-infection, Tissue tropism