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Home > Diagnostic Accuracy of a Commercial Cross Priming Amplification Diagnostic Kit in Smear Negative Sputum

Diagnostic Accuracy of a Commercial Cross Priming Amplification Diagnostic Kit in Smear Negative Sputum

Thesis Info

Author

Makau, Paul M.

Department

Pathology (East Africa)

Program

MMed

Institute

Aga Khan University

Institute Type

Private

City

Karachi

Province

Sindh

Country

Pakistan

Thesis Completing Year

2012

Thesis Completion Status

Completed

Subject

Medicine

Language

English

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676728044586

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Background: The diagnosis of smear negative tuberculosis remains a major obstacle to global control of tuberculosis (TB), especially in resource limited settings. Several nucleic acid based diagnostic tests have been developed to improve diagnosis. The polymerase chain reaction has been the benchmark for nucleic acid amplification; many novel alternatives have been developed for the amplification and detection of nucleic acid sequences. Objective: The study aimed to determine the diagnostic accuracy of Cross Priming Amplification technology using the Ustar TB Isothermal Amplification Diagnostic (IAD) Kit (Ustar Biotech, Hangzhou, China) in smear negative sputum. Methods: 159 unconcentrated sputum samples negative by direct Ziehl-Neelsen staining referred to the National Central Reference Lab in Nairobi were subjected to both the Ustar TB Isothermal Amplification Diagnostic Kit and culture. The reference standard was TB culture. Results: When tested on Ziehl-Neelsen negative smear sputum, the IAD assay was found to have a sensitivity of 75% (60-86%; 95% CI); Positive Predictive Value (PPV) of 84% (71-94%; 95% CI) and Diagnostic Odds Ratio of 42.18 (15.65-113.69; 95% CI). The specificity of the assay was 94% (87-97%; 95% CI). Conclusion: The assay demonstrated good sensitivity and moderate specificity in detecting mycobacterium tuberculosis in smear negative specimen, the requirements for a clean room for the DNA extraction procedure, a high speed centrifuge and biological safety chamber may limit the applicability of this technique in resource limited settings.
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حکیم محمد سعید دہلوی

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کس قدر ظالم ہاتھ تھے اور کس قدر درندہ صفت دل کا وہ انسان نہیں حیوان رہا ہوگا جس نے فرشتہ صفت ہستی، غریب انسانوں کامسیحا، ملک وملت کاخیرخواہ ،پوری انسانیت کی فلاح وبہبود گی چاہنے والا،شرافت کاپیکر مجسم، حکیم محمدسعید دہلوی پر گولیاں چلا کر آن کی آن میں ان کو شہید کردیا۔اناﷲ وانا الیہ راجعون۔
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PENDIDIKAN ISLAM TERPADU: SEBUAH KAJIAN TEORITIK

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