ذولفقار علی بھٹو کی سپریم کورٹ میں آخری تقریر
بعد ازاں اپیل کنندہ کے ذریعے عدالت میں ایک تحریری درخواست بھی پیش کی گئی تھی جس میں اس معاملے کے کچھ پہلوئوں پر ذاتی طور پر عدالت سے خطاب کرنے کے موقع کی درخواست کی گئی ۔ اس درخواست کی اجازت دی گئی اور اسی مناسبت سے اپیل کنندہ 1978 ء میں 18 سے 21 دسمبر تک ذاتی طور پر چار دن عدالت میں پیش ہوا ۔ اپیل کنندہ ذوالفقار علی بھٹو نے تقریباً بارہ گھنٹوں پر پھیلے ہوئے وسیع خطاب میں اپنے اوپر لگائے گئے الزامات سے انکار کیا جو کہ بے مقصد، مستغیث اعلیٰ احمد رضا قصوری نے قتل کے گواہ ہونے سے متعلق لگائے تھے۔ اس وقت کے فیڈرل سکیورٹی فورس کے ڈائریکٹر جنرل اور گواہ مسعود محمود جنھوں نے یہ بھی دعویٰ کیا کہ شواہد میں سازش کے جرم کے کسی ضروری اجزا کی موجودگی کا انکشاف نہیں کیا گیا۔ یعنی متفقہ طور پر شریک سازشیوں خصوصاً مسعود محمود کی طرف سے جس نے اپیل کنندہ کی جانب سے سختی سے استدعا کی تھی اپیل کنندہ نے اس پر تبصرہ کیا کہ گواہوں ،احمد رضا قصوری اور مسعود محمود کے ثبوت میں موروثی تضادات موجود ہیں اور عرض کیا کہ وہ ملک میں مارشل لاء کی مجبوری کے تحت کام کر رہے ہیں۔ انھوں نے کہا کہ اگر استغاثہ یہ چاہتا تھا کہ عدالت ملک میں بطور صدر اور وزیر اعظم اپنے عہدے کے دوران پاکستان میں موجود مبینہ معاشرتی حالت کا عدالتی نوٹس لے تو پھر اس طرح کا نوٹس بھی اس حقیقت پر لیا جانا چاہیے جو مارشل لاء کے تسلسل کے دوران مقدمے کی سماعت میں ثبوت اہم گواہ دے رہے تھے۔ انھوں نے سختی سے استدلال کیا کہ ان کے خلاف سارا مقدمہ جھوٹا اور من...
The goal of this investigation is to discover how Kamila Shamsie's book "Home Fire" has been misinterpreted and used about the masculinity crisis for identity and existence. This study explains how the artificial masculinity imposed by society and culture on the male characters in the novel is forced onto them, only for self-satisfaction. The purpose of the study is to highlight the fact that different cultures, particularly the dominant diasporic culture, have distinct beliefs about masculinity and femininity in terms of identity and outright denial. The author supports this by pointing out how these masculinities also repress women. Erikson's work on identity, which is being examined under psychological views and the restraint of other creatures, has an impact on this study. By integrating self-categorization with presumptions about the nature of intergroup relationships, social categorization depersonalizes perception, cognition, effect, and behavior in terms of relevant in-group or outgroup prototypes and explains particular instances of group behavior. This project is done under qualitative data of research, however, in the conclusion; it is assessed how the Pakistani community is expressing its anxiety about masculinities
Leptochloa fusca L. is a halophyte plant from the Poaceae family and is also locally known as kallar grass. It is a perennial, summer-growing, forage grass that is now cultivated in many parts of the Pakistan and India. It is highly tolerant to salinity, sodicity, water logging and high soil pH. Plants have evolved a variety of adaptation mechanisms against salt stress, such as; restricting the uptake of environmental Na + ; increasing the efflux of Na + from the cell; and sequestering Na + into the large intracellular vacuole to reduce Na + accumulation in the cytosol. Compartmentalization of the Na + into vacuoles can be accomplished by the action of Na + /H + antiporters in the vacuolar membrane. They catalyze the exchange of Na + for H + across tonoplast membranes using the proton gradient generated by the vacuolar H + -ATPase and H + -pyrophosphatase (PPase). The function of the NHX1 antiporter depends upon free H + provided by protons pumps. The objectives of the study were to isolate H + -pyrophosphatase and Na + /H + antiporter genes from Leptochloa fusca and characterize for salt and drought tolerance. During present study the full length H + - PPase and NHX1 genes were isolated from cDNA of L. fusca using degenerate primers generated from corresponding sequences of plants of the Poaceae family. The L. fusca H + -PPase (LfVP1) cDNA contained an uninterrupted open reading frame of 2,292 bp, coding for a polypeptide of 764 amino acids. The LfVP1 sequence showed 91% identity with Z. mays H + -PPase, 90 % with S. bicolor and 80 % with A. thaliana H + -PPase. The L. fusca NHX1 cDNA contained an uninterrupted open reading frame of 1,623 bp coding for a polypeptide of 541 amino acids. The L. fusca NHX1 gene sequence showed 88 % identity with S. bicolor and 87 % with Z. mays genes. The LfVP1 and LfNHX1 genes were characterized using various online bioinformatics tools; hydrophobicity plots; 2 dimensional transmembrane structures; and protein 3 dimensional structures. The LfVP1 and LfNHX1 genes were cloned under the control of Gal promoter in a Gateway ® yeast expression vector and transformed in rg9 (control); ena1 and ena1;nhx1 yeast mutants. Yeast complementation assay on hygromycin plates showed that overexpression of the LfVP1 and LfNHX1 genes suppressed the hygromycin susceptibility phenotype in yeast mutants. The LfVP1 and LfNHX1 were also cloned under control of the 35S, 2X-35S, ZmUbi and OsAct promoters using the Gateway ® technology. The LfVP1 and LfNHX1 genes were transferred in tobacco through Agrobacterium mediated plant transformation under xvicontrol of the 35S promoter and characterized for salt and drought tolerance. The LfVP1 and LfNHX1 transgenic lines showed higher levels of relative water contents, stomatal conductance, net photosynthetic rate, membrane stability index and more negative value of leaf osmotic potential as compared to wild type control plants. The LfVP1 and LfNHX1 transgenic plants were able to germinate and maintain their growth at to 200 mM and 250 mM NaCl. The LfVP1 and LfNHX1 transgenic plants also showed better germination at 2 mg L - Basta ® ( glufosinate-ammonium ) . The LfVP1 and LfNHX1 genes were also transformed in wheat under ZmUbi promoter. Putative transgenic plants were confirmed through PCR amplification and leaf bioassays.