مولانا احمد علی
افسوس ہے پچھلے دنوں مولانا احمد علی اورمسٹر شعیب قریشی نے داعی اجل کولبیک کہا اوررہ گزائے عالم جاودانی ہوگئے۔مولانا احمدعلی حلقۂ دیوبند کے اکابر و مشائخ میں سے تھے۔بلند پایہ عالم، وسیع النظر مفکر اوردرویش صفت بزرگ ہونے کے علاوہ اونچے درجہ کے صاحب معرفت وباطن بھی تھے۔قرآن مجید کی تفسیر اوراُس کی تعلیم وتدریس سے خاص شغف تھااوراُس کا بڑااہتمام کرتے تھے، دارالعلوم دیوبند اوردوسرے مدارس عربیہ کے فارغ التحصیل طلبا اسی غرض سے لاہورجاتے اورچند ماہ قیام کرکے مولانا کے مخصوص درس قرآن سے مستفید ہوتے تھے۔پنجاب کے انگریزی تعلیم یافتہ حضرات بھی موصوف کے زیر اثر تھے اوراُن کی اچھی خاصی تعداد اس درس میں پابندی سے شریک ہوتی تھی۔ پنجاب میں مولانا کی ذات جواحیاء دینی،روحانی واخلاقی تعلیم وتربیت اورنشروتبلیغ علوم ومعارف قرآنیہ کے لیے وقف تھی۔ مرجع عوام وخواص تھی۔تقریر موثر اور دلپذیر ہوتی تھی مگر مجلس میں کم سخنی وکم گوئی اُن کی خو تھی۔زندگی بڑی سادہ تھی، تکلف، تصنع اورمادی آرائش وزیبائش سے نفرت تھی۔ان خوبیوں کے علاوہ مجاہد فی سبیل اﷲ بھی تھے۔تحریک خلافت اوراُس کے بعد جدوجہد آزادی میں ہمیشہ جمعیۃ علماء ہند کے ساتھ رہے۔مولانا عبیداﷲ سندھیؒ کے داماد تھے۔اس تعلق سے اُن کومولانا سے استفادہ کا زیادہ موقع ملا تھااوراُس کااثر اُن کے درس قرآن میں اورعام تقریروں اورگفتگوؤں میں بھی ظاہرہوتاتھا۔قیام پاکستان کے بعد سے بڑے بڑے اہم مسائل رونما ہوئے اوربعض اوقات علماء کے لیے سخت ابتلا و آزمائش کے مواقع پیداہوگئے، مگرمولانا نے اعلان حق اوراعلائے کلمۃ اﷲ میں کبھی بھی کوتاہی یاپہلوتہی سے کام نہیں لیا۔اُن کواس کی سزابھگتنی پڑی۔مگر انھیں اس کی کبھی پروا نہیں ہوئی۔اب ایسے علمائے حق کہاں ملیں گے؟جوجاتاہے اپنا صحیح جانشین وقائم مقام چھوڑ کرنہیں جاتا۔ اللھم بردمضجعہ وارحمہ رحمۃً واسعۃً۔ [اپریل۱۹۶۲ء]
Great lineage, good manners, trustworthiness, sincerity, courage and bravery, obedience, generosity, modesty, humility, thankfulness, absolute trust in Allah, education and upbringing, affection and compassion towards the elderly, the youth, slaves and animals, the regard for truth, the endurance and patience etc. Are such features that at them neither was the match for the Holy Master (saw) born, nor will any such person be ever born. In addition, let alone friends, his enemies were helpless to hold him as-Sadiq (the Truthful) and al-Ameen (the Trustworthy) due to these and many other great attributes; and they would say: Muhammad is not a liar at all, but we can’t believe in him as a prophet sent by Allah. Moreover, even a non-Muslim or polytheist has never criticized his character to this day, nor will anybody do till the judgment day. Whenever the greatest personalities of the world were listed or somebody committed to writing on the said topic, the Holy Prophet (saw) remained at the top of the list. Drawing of the swords of father and son against each other, the change of the standard of friendship and enmity, fighting of master and his slave against each other, chanting the slogan of Ahad-Ahad (the only One, the only One) on the blazing sand, giving priority to martyrdom over the utterance of a word of unbelief, taking a husband away from his wife and separating a mother from her infant, deforming the condition after a blasphemous blow on the blessed face, forced migration to Habsha (Abyssinia) at times, and laying siege to Shb-e-Abi Talib (the valley of Abi Talib) at times.
Staphylococcus aureus is the main cause of mastitis in dairy goats. This organism is recognized as major mastitis causing pathogen in dairy animals worldwide. In humans, it also caused various diseases such as food poisoning and Staphylococcal enterotoxins. This bacterium mostly infected the dairy herds due to poor sanitary conditions and untreated groundwater used to wash utensils during milking practice. The purpose of this study was to determine the prevalence of Staphylococcus aureus among infected dairy goats. Hundred milk samples were collected from infected dairy goats and grown on a blood agar. A total of 58/100 (58%) were positive for S.aureus that was further confirmed on the basis of biochemical tests namely catalase positive and coagulase positive. The positive isolates of S. aureus strains were analyzed using a molecular technique. DNA of positive S. aureus isolates was extracted using Macherey-Nagel kit method. The extracted isolates of S. aureus were run on agarose gel electrophoresis. Ethidium bromide dye was used to visualize DNA bands using UV illuminator. The amplification of genes encoding 16S rRNA of positive S. aureus isolates was performed by Polymerase chain reaction. Primers (Forward primer 5''-AGA GTT TGA TCC TGG CTC AG & Reverse primer 5''-AAG GAG GTG ATC CAG CCG CA) were designed using Primer 3 software. PCR clean up method was used in purification of sequences. These amplified gene products were further sequenced by using Sanger method. The sequenced results of these bacterial species were confirmed by comparing the sequences present in BLAST program at National Centre of Biotechnology Information. MEGA 7 software was applied for the construction of Phylogenetic tree. Some other bacterial species such as Staphylococcus epidermidis, Staphylococcus hominis, Enterobacter cloacae and Acinetobacter baumannii along with Staphylococcus aureus were also identified due to their genetic differences. The evolutionary history was inferred using the Neighbor-Joining method. The evolutionary distances were computed using the p-distance method and in the units of the number of base differences per site. The analysis involved 14 nucleotide sequences. There were a total of 371 positions in the final dataset. Antibiotics such as penicillin, erythromycin or tetracycline are used in the treatment of mastitis in goats. It is concluded that Staphylococcus aureus was more prevalent in the milk samples of infected goats and is an etiologic agent of mastitis in dairy goats. The consumption of raw milk may infect humans and lead to various infections.