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Home > Identification and Characterization of Antioxidant and Cytotoxic Activities of Selected Medicinal Plants of Gallyat Region, Pakistan

Identification and Characterization of Antioxidant and Cytotoxic Activities of Selected Medicinal Plants of Gallyat Region, Pakistan

Thesis Info

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Author

Ishaque, Muhammad

Program

PhD

Institute

Pir Mehr Ali Shah Arid Agriculture University

Institute Type

Public

City

Rawalpindi

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/11297/1/Muhammad%20Ishaque_Botany_2018_PMAS_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676728105921

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Human have always relied on biological products and have continuously explored their potential to improve various aspects of humanlives.The research on natural product is one of the key of discovering bioactive natural substances. Since with little knowledge about the etiology of many human, animals and plants disorders, this become difficult to synthesized potentially active substance in the chemical laboratory for their treatment. The bioactive compounds obtained from natural sourcesprovideharmonizingattributes to synthetic compounds with respect to their composition, size, different functional groups, architectural and stereo chemical complexity. Importantly, these bioactive natural substances have potential to serve functions in specific biological system like scavenging of free radicals, inhibiting the tumor etc.Medicinal plants have been playing a vital role in combating various ailments including cancer. Many modern antitumor and anticancer drugs have plant-based origin. e.g.Podophyllotoxin (Podophyllumpeltatum), Vincristine (Catharanthusroseus) etc.Study area i.e. Gallyat region has rich diversity of medicinal plants. Five ethnomedicinally important plant species (Dryopterisramosa, Quercusleucotricophora, Bidensbiternata, Arisaemaflavum and Rosa brunonii) were selected from the study which were never been assessed for their antioxidant and cytotoxic potential. The selected plant species were collected from Gallyat region of Pakistan, identified and voucher specimens were deposited in herbarium of Quaid-i-Azam University Islamabad for future references.Crudemethanolic extract (CME) of each selected plant was prepared using maceration technique and each plant extract was evaluated for its antioxidant xxx and cytotoxic potential by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, Brine shrimp lethality (BSL) assay and Potato disc antitumor (PDA) assay respectively.The CME of D. ramosa showed maximum free radical scavenging potential (IC5088.67±0.73 µg/mL) followed by CME of Q. leucotricophora(IC5095.51±0.19µg/mL)and R. brunonii (IC50131.41±0.18 µg/mL). CME of A. flavum and B. biternata showed minimum free radical scavenging potential. The crude extract of R. brunonii showed highest cytotoxicity against Brine shrimps than all other crude extracts at all concentrations tested.The maximum antitumor potential was shown by crude extract of R. brunonii(IC50 655.65µg/mL) followed by D. ramosa (IC50 790.51µg.mL) and A. flavum (IC50 825.94 µg/mL).The CME of D. ramosa and R. brunonii were subjected to polarity based solvent-solvent fractionation. All fractions [n-hexan fraction (nHF), Chloroform fraction (CF), Ethl acetate fraction (EF) and Aqueous fraction (AF)] so obtained were assessed for antioxidant and cytotoxic potential. The maximum antioxidant potential was showed by EF of D. ramosa (IC50 57.85±0.24µg/mL) followed by AF of same plant (IC50 108.98±0.28µg/mL). The EF of R. brunonii showed maximum cytotoxicity (LD50405.43±4.8µg/mL) followed by AF of D. ramosa (LD50830.95±2.0µg/mL). The maximum tumor inhibition in PDA was showed by EF of R. brunonii(753.68±0.48µg/mL) followed by AF of D. ramosa (793.23±0.31µg/mL) and EF of D. ramosa (834.99±0.24µg/mL). In comparison with control, the vincristine a well-known antitumor pure compound showed IC50 232.34±0.58µg/mL. The EF and AF of D. ramosa and EF of R. brunonii were subjected to different chromatographic techniques (CC, HPLC, TLC, PTLC etc.) in order to isolate pure compounds. Iriflophenone-3-C-β-D- glucopyranoside, xxxi Mangiferrin, iso-mangiferrin and Astragalin were isolated from selected fractions D. ramosawhile Quercetin-3-O-rhamnoside, Kampferol-3-O-β-glucopyranoside and Tilirosidewere isolated from ethyl acetate fraction of R. brunonii. The structure of isolated compounds were determine by UV-Vis spectroscopy, MS, FT-IR, 1-D NMR (1H, 13C, DEPT) and 2-D NMR (COSY, HSQC, HMBC). All the isolated compounds showed high antioxidant potential except tiliroside (IC50923.67± 0.86µg/mL) while tiliroside showed excellent cytotoxic potential (LD5050.42± 0.88µg/mL). This is the first report of antioxidant and cytotoxic activities and isolation of compounds from D. ramosa and R. brunonii to the best of our knowledge. Further in-vivo and in-vitro testing of these isolated compounds is recommended. They may prove to be the potential future drugs.
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شاہ آفاق احمد ردولوی

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درگاہ شریف، ردولی۔
محترمی! تسلیمات
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Nawaz Sharif’s Speeches to the United Nations General Assembly: A Corpus-Based Analysis

Every year heads of different states make their speeches in the general debate of United Nations General Assembly (UNGA). They address national and international issues and suggest measures to resolve them with the help of international community and the United Nations. Muhammad Nawaz Sharif also made speeches in the UNGA. In 2013, he was newly elected prime minister and was enthusiastic to resolve issues with India. There was no internal political pressure on him at that time and the government in India was hostile towards Pakistan. But one year in power and embattled with domestic issues and international pressure, the prime minster approached the issues differently in his 2014 speech. The aim of this paper is to compare his two speeches and see what changes occurred in his stance towards India. The main focus of the analysis is: Who is the addressee of these speeches? How confident did Nawaz feel about the solution of the issues that he raised. The Corpus tool Wmatrix has been used to analyze the data. The results show significant differences in his two speeches with regard to his stance towards India.

Metallic Phytoremediation and Nanobiotechnology of Water Hyacinth

Like world Pakistan is facing two major threats i.e. environment and energy. Rapid industrialization and urbanization have resulted in elevated emission of toxic heavy metals entering the biosphere. Activities such as mining and agriculture have polluted extensive areas throughout the world. The release of heavy metals in biologically available forms by human activities may damage or alter both natural and man-made ecosystems. Heavy metal ions such as Pb 2+ , Cr 6+ , Cd 2+ Cu 2+ , Zn +2 and Ni +2 , are essential micronutrients for plant’s and animal’s metabolism but when present in excess, can become extremely toxic. Among the renewable energy technologies, solar energy, wind energy, biofuels and biomass conversion occupy the central stage. The current study belongs to biomass conversion to biofuels. It was focused on metal phytoremediation and biofuel production from water hyacinth by using Nanobiotechnology. Soil experiments show the phytoremoval of Pb 2+ , Cr 6+ , Cd 2+ Cu 2+ , Zn +2+ and Ni 2+ in μg/g of plant’s dry weight. Increase or decrease in pH and fresh weight is also discussed. Lead, chromium, cadmium, copper, zinc and nickel showed phytoremoval of 27.91, 38.67, 1.915, 3.38, 13.11 and 24.23 μg/g of plant’s dry weight respectively. Similarly phytoremoval of lead, chromium, cadmium, copper, zinc and nickel in kg/ha is 1582.45, 1053.38, 2852.75, 232.99, 1521.08 and 3449.76 respectively. Hoagland’s hydroponic experiments show the phytoremoval of Pb 2+ , Cr 6+ , Cd 2+ Cu 2+ , Zn +2+ and Ni 2+ in μg/g of plant’s dry weight. Increase or decrease in pH and fresh weight is also discussed. Lead, chromium, cadmium, copper, zinc and nickel showed phytoremoval 19.132, 2.369, 8.888, 4.085, 0.845 and 1.954 μg/g of plant’s dry weight. Adsorption and desorption experiments show phytoremoval (adsorption) of Pb 2+ , Cr 6+ , Cd 2+ Cu 2+ , Zn +2+ and Ni 2+ in μg/g of plant’s ash. While desorption (recovery for reuse) of lead, chromium, cadmium, copper, zinc and nickel in μg/g of plant’s ash. Adsorption capacity is 29.83, 24, 28.41, 29.83, 29.94 and 29.79 μg/g of plant’s ash respectively. The biosorptive capacity is highest with pH > 8.00. The desorption capacity of lead, chromium, cadmium, copper, zinc and nickel is 18.10, 9.99, 27.54, 21.09, 11.99, 3.71 μg/g of plant’s ash respectively. Bioanalytical experiments were performed to assess the metallic concentrations of Taxilian water hyacinth. Some selected microbes from hyacinth’s roots were isolated, xxidentified by biochemical tests and purified by microbiological experiments. Our results show that Thiobacillus thiooxidans, Thiobacillus ferrooxidans, Azotobacter and A. niger are present in the roots of water hyacinth. Thiobacillus thiooxidans, Thiobacillus ferrooxidans and A. niger are commonly well known for metal’s bioremediation. Their presence in the roots of water hyacinth show that these microbes may have some role in metal’s phytoremediation by hyacinth. Azotobacter is generally nitrogen fixing bacteria its presence may shows relationship with nitrogen’s phytoremoval by hyacinth. The presence of Mn, Mo, Fe, Cr and some other metals in nitrogenase enzymes of Azotobacter, is already documented. Therefore Azotobacter’s presence may also be responsible for phytoremoval of these metals by water hyacinth. Three different studies were performed for the nanobiotechnological conversion of water hyacinth (Eichhornia crassipes) plant into biofuel. In the first study water hyacinth was saccharified with diluted sulfuric acid (1% v/v at 110 o C for one hour) and fermented by yeast (Saccharomyces cerevisiae). The results show the formation of 55.20 % ethanol and 41.66 % acetic acid. In another experiment water hyacinth was gasified by using Ni and Co nano catalysts at 50-400oC and atmospheric pressure. In catalytic gasification methane (2.41-6.67%), ethene (19.74-45.52%), propyne (21.04-45.52%), methanol (1.43- 24.67%), and propane/acetaldehyde (0.33-26.09%) products are obtained. In third study anatase form of titanium dioxide photocatalyst was used. The reaction was performed at room temperature which gives good percentage of methane (53.19%), methanol (37.23%) and ethanol (9.57%). This study reports an interesting finding that metal contaminated water hyacinth could be used for not only the production of biofuel but also hydrocarbons Present study gives solution of two major problems of the world i.e. environmental pollution and fuel. It also shows comparison of three different phytoremediation technologies and use of water hyacinth as a metal hyper accumulator and a source of hydrocarbon gases and bioethanol. These technologies are cheap and may be developed further for commercial use.