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ڈاکٹر مقصود جعفری
کب تک رہِ اُلفت پہ تُو نادان رہے گا؟
اِس رہ پہ چلے گا تو پریشان رہے گا
اب بارِ محّبت کو سفینے سے اتارو
ورنہ یہ سفینہ تہہِ طوفان رہے گا
ہر دور کے آمر سے لڑائی رہی میری
ہاتھوں میں مرے اِن کا گریبان رہے گا
اِن دین فروشوں میں ہے اوہام پرستی
اِنساں کی اسیری میں ہی اِنسان رہے گا
یہ بارشِ الہام تو ہوتی ہی رہے گی
جب تک میرے اشعار میں وجدان رہے گا
کیا اہلِ جُنوں عقل و خرد کے ہوۓ دشمن ؟
کیا کلبۂ ادراک بھی سُنسان رہے گا؟
دربانی مجھے آتی نہیں جعفری صاحب
دربار کا دربان ہی دربان رہے گا
فیروز ناطق خسرو
جو ووٹ دوسروں کے تھے آپس میں بٹ گئے
چشمِ زدن میں سارے نتائج اُّلٹ گئے
اپنی طرف سے کچھ بھی اضافی نہیں کہا
جو لفظ بھی رٹائے گئے تھے وہ رٹ گئے
پوچھے کوئی کیوں اپنی صفیں روندتے ہوئے
پورس کے ہاتھیوں کی طرح وہ پلٹ گئے
محروم اپنے حق سے جو ناحق کیے گئے
مظلوم ظالموں کے مقابل میں ڈٹ گئے
سورج نئی سحر کا نمودار ہوگیا
بادل جو گِھر کے آئے تھے کالے وہ چھٹ گئے
دھندلا دیے ہیں وقت نے اپنے بھی خد و خال
آئینہ رخ بھی دھول میں لمحوں کی اٹ گئے
خسروؔ لکھیں گے نوکِ مژہ سے بھی حالِ دل
چھینا گیا قلم جو کبھی ہاتھ کٹ گئے
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Objective: The objective of the study is to explore the relationship between body image, self-esteem and academic behavior amongst adolescent girls living in Karachi, Pakistan and simultaneously explore the mediating role of self-esteem in the relationship of the other two variables. Methods: It was a cross-sectional study design with a sample size of 400 adolescent girls belonging to a selected community of Karachi, Pakistan. Pre-validated tools, Body Shape Questionnaire, Rosenberg Self-Esteem Scale and Academic Behavior Scale were used for data collection of the study. The data was then analyzed using SPSS 17. Results: The results proved significant associations between all three variables with p<0.05. Moreover, a partially mediating role of self-esteem was noticed to be causing 74.8% variation in the relationship between body image and academic behavior. Conclusion: As per the study findings, body image, self-esteem and academic behavior of adolescent girls residing in Karachi, Pakistan are associated with each other. It was also concluded that higher body image dissatisfaction may lead to poor self-esteem which in turn negatively impacts the positive academic behavior of the girls. Hence, representing a mediating role of self-esteem in the relationship. Overall, parents, educators, academic professionals, counselors and healthcare professionals can benefit from the study findings to positively play their parts in improving the lives of adolescents around them.
Enzyme immobilization utilizing distinctive methodologies and different supports appears to be the most effective possibility for developing industrially compatible enzymes. Immobilization has been discovered as a capable device for enhancing all catalyst properties, specific activity, thermo stability and decreasing inhibition. Laccase with improved catalytic features is a potentially attractive biocatalyst with many possible applications including bioremediation, biomass delignification for (590706) 4.58 Visual comparison of decolorization of dyes with free and immobilized laccase with chitosan bead (a) Sandal-fix Red C4BLN (b) Sandal-fix Golden yellow CRL (300410) 138 4.59 Visual comparison of dye degradation (A) control (B) degradation with free Laccase (C) degradation with laccase immobilized with PVA beads (D) degradation with laccase immobilized with Ca-Alginate beads 140 4.60 Repetitive use of immobilizedlaccase for dye decolorization 142 4.61 Storage stability of free and immobilized Laccase at 16oC 144 ethanol production, chemical synthesis, bio pulping and biosensor development, denim stone washing, cosmetics, dyes degradation, textile finishing and wine stabilization. In this study, four copper containing extracellular laccase was purified from culture filtrate of an indigenous WRF strain Trametes Versicolor IBL-04, grown in solid state fermentation (SSF) under pre optimized condition using corncobs as a substrate. The enzyme was purified (1.00-4.03 fold) to apparent electrophoretic homogeneity through ammonium sulphate precipitation, dialysis, DEAE-cellulose ion exchange and Sephadex G-100 gel exclusion chromatography. The purified enzyme elucidated a monomeric single band on Native and SDS polyacrylamide gel electrophoresis (SDS-PAGE) with an apparent molecular weight of 66 kDa. With an aim to improve its functionalities, the purified laccase was effectively immobilized on nine supporting matrices using diversified immobilization techniques and the relative activity at optimum temperature and pH and thermal stability of free and immobilized laccase were studied for comparative analysis. Scanning electron microscopy (SEM) and Transmission electron microscopy (TEM) were performed to characterize enzyme immobilization. Immobilized laccase was successfully used for the decolorization of six different synthetic (textile) dyes with more than 98% removal rate. The resulted modified laccase enzyme was reusable for up to many cycles in dye color removal and also check the storage stability and thermostability of immobilized laccase. All immobilized parameters showed that immobilized laccase is better for industrial use in comparison to free laccase. Finally results of this study suggested that immobilized laccase catalytic system can be efficiently exploited for dye degradation due to its sustainability, durability and reusability over its soluble counterpart. Keywords: Trametes Versicolor IBL-04, Laccase, purification, characterization, immobilization, dye decolorization