غزل---ڈاکٹر انور علی انور
جنگ میں قائل رہا ہوں کب میں تیغ و تیر کا
اک زمانے سے رہا ہوں بر سر پیکار میں
برف باری کا یہ موسم درد کی تصویر ہے
میں نے اپنا دل جلایا ہے تمھارے واسطے
قتل کرکے وہ مجھے یاروں سے یہ کہتا رہا
میری چاہت کا محل مسمار ہوتا ہی رہا
جس نے میرا راستہ روکا ہے انور کیا کہوں
مانتا ہوں پھر بھی انور رول میں شمشیر کا
یہ قلم بھی اک حوالہ ہے میری توقیر کا
اور میں مارا ہوا ہوں درد کی تصویر کا
اور تم نے طوق پہنایا مجھے رہ گیر کا
کیا خطا میری ہے اس میں کھیل ہے تقدیر کا
آگرا سر پر میرے ملبہ میری تعمیر کا
اک سرا میں نے بھی تھاما ہے اسی زنجیر کا
The foundation of the Sharia is revelation, revelation is the name of two things, the Qur'an and the Sunnah, since both are related to the news, and to convey the news to others, narrators are needed, so for the propagation of the Qur'an and the Sunnah to future generations. It was necessary to have narrators, the narrators of the Holy Qur'an are called Qira, the narrators of the Sunnah are called Muhaddith, the traditions of the Holy Qur'an are called 'Qara'at' and the traditions of the Sunnah are called 'Ahadith'.
Both the Qur'an and the Sunnah are revelations, but still there are some differences between them which are explained in detail in the Book of Principles. It was a difficult task, and the significant efforts made by the Muhadditheen in this regard were more famous and campaigned than the knowledge of al-Qaraat and recitation. He became famous with this, and some people even got the wrong impression that he had nothing to do with jurisprudence, and this wrong impression was reinforced by the behavior of the some Narrators.
In reasoning and deriving from the Sunnah, there were many disorders and factors that gave birth to different schools of jurisprudence. For example, a hadith revealed to an imam or a jurist during reasoning has a hidden reason that is not revealed to anyone else. Therefore, there is a difference in argumentation. Similarly, sometimes the hadeeth is correct in a certain issue in front of a jurist, while on the other hand, it is weak in the opinion of another, which leads to diversity in argumentation.
When the jurists differed in the derivation of the issues and rulings, in fact, these are cases of priority and non-priority, in which there is, however, scope that any position can be declared preferred based on arguments.
Keywords: Hadith, Muhaddithin, Jurisprudential Proverbs, School of Thoughts, Differences.
The bacterium Bacillus thuringiensis (Bt) produces Cry toxins that possess toxic properties and can be used as biopesticides. Cry2Aa and Cry2Ac are among unusual subset of crystalline proteins possessing broad insect species specificity by exhibiting high specific activity against larvae from two insect orders, Lepidoptera and Diptera of agricultural and public health significance. The cry2Ac11 gene is located at third position (orf3) in operon comprising of three genes. It needs accessory proteins for crystal formation and high yield. Translation initiation is key rate-limiting step. It is well-known that stable structure at a ribosome binding site (RBS) impedes initiation. Modification in RBS-spacer region tunes translation initiation rates. Genetic manipulation of cry2Ac11 gene without helper protein was carried out in this study by optimizing ribosomal binding site and spacer region (RBS-ATG) in translation initiation region (TIR). The five different types of mutations were introduced in TIR to unveil inhibitory and excitatory effects on translation. These mutants are: 1), operSalI/RBSD and mut/RBSD in which downstream RBS (GGAGG) 6 bp downstream to native RBS was introduced in TIR of cry2Ac11 operon and gene; 2), mut/RBSF in which four nucleotides (ATGGG) were incorporated after RBS-ATG spacer region; 3), mut/RBSSin which overlapping start and two stop codons were introduced after RBS-ATG spacer region; 4), mut/RBSSP in which RBS-ATG spacerregion waslengthened to 23 nucleotides; 5), mut/RBS2 in which consecutive two ATGs were incorporated in TIR. Secondary structures of mutants, estimated by CLC Main Workbench, revealed that mut/RBS2 RNA exhibits most stable structure in RBS-AUG region. RBS Calculator predicts high translation rate in mut/RBSD and mut/RBS2. Mutants were expressed in B. thuringiensis 4Q7 acrystalliferous strain. The transcriptomics-proteomics profiles of all cry2Ac11 constructs provide a unique opportunity to investigate how faithfully the transcriptional profile is manifested at the protein level. Therefore, in this study correlation between mRNA abundance and protein expression profiles in all Cry2Ac11 recombinant strains were also investigated. The highest transcript profile of B. thuringiensis 4Q7-mut/RBS2, (a mutant in which consecutive multiple AUG were introduced), was obtained by Real time PCR. Furthermore, SDS-PAGE profile of total cellular proteins indicated that overexpression of Cry2Ac11 (65kDa) was obtained in 4Q7-mut/RBS2. It was concluded that overexpression of Cry2Ac11 toxin without helper protein in mut/RBS2 mRNA was most likely due to presence of consecutive start codons (AUGs) in TIR. Presence of RBS in the single stranded part of moderately stable hairpin loop (ΔG = 8.7 kcal/mol) in mut/RBS2 facilitates the interaction of RBS to the complementary 16S rRNA sequences of 30S ribosomal subunit. In proposed model, multiple factors are thought to contribute in translation efficiency of mut/RBS2 (cry2Ac11 mutants without helper protein) which includes stabilizer sequence at 5′ and 3′ ends, the availability of the RBS for binding to the anti-SD of 16S rRNA of 30S ribosomal unit and optimal context of RBS-AUG region provided by multiple AUGs in TIR.