Significance of Philadelphia Chromosome in Chronic Myeloid Leukemia Patients of Anmol Hospital, Lahore, Pakistan

Background: Chronic myelogenous Leukemia is a form of cancer that was firstly recognizes to associate strongly with the chromosomal abnormality [t (9; 22) translocation] called Philadelphia chromosome. Objective: Philadelphia chromosome is a characteristic chromosomal marker that is associated with chronic myelogenous leukemia. Methods: More than one hundred patients of either sex were selected for the experiment. RNA was isolated from whole blood of patients so can use exclusively in RT-PCR. Results: Philadelphia chromosome in blood samples of patients with suspected diagnosis of CML was detected in 63% of patients. During our experimental studies on CML patients we do not encounter any complex translocation involving chromosome 8, 9 and 22.  Conclusions: Philadelphia chromosome is a precise cytogenetic marker the detection of which is significant for differential diagnosis and clinical organization of patients with clinical diagnosis of CML. It is of significant that Ph chromosome occurs in pre-leukemic stage and has great diagnostic significance.

Socioecological Study of Mangla Lake With Inter-Disciplinary Management of its Biotic Resources

Mangla Dam of Azad Jammu and Kashmir is the 9th biggest dam of the world. This deep fresh water lake is located (33.12 N, 73.39 E) 30 Km North West of Punjab province in Pakistan and is spread over an area of 265 Km2 emerged as important waterfowl habitat. The basic aim of sustainable use of biotic resources is to accomplish the demand of the humans and safeguarding the reserves not for today but also for future generations. The objectives of the current work were collection of socioecological data with the aim to write up a sustainable management plan, documentation of biotic resources, monitoring of avian diversity and identification of threats with their root causes to the biological diversity of Mangla dam. To access the socioecological profile of communities Participatory Human Resource Interaction Appraisals (PHRIA methodology) was applied. A total of 17 surveys form May, 2011 to April, 2014 were conducted to collect the data from 392 individuals (158 females and 234 males) of seven villages. The site was visited 42 times from 2011-2014 to estimate the biodiversity and population density of avian fauna with point count method. It was analyzed that the resources of Mangla wetland contributed to household economy of local people at considerable level. The results of community surveys indicated that management of resources without community involvement is out of question. The floristic composition in catchments and around Mangla dam was consisting of 163 plant species including trees, shrubs, herbs and grass. The fauna of the site consisted of 42 species of fish. The amphibians (n=2) and reptiles (n=12) species were observed at study site. At the site 13 species of mammals were identified which were 7.5% of 174 described mammals species of Pakistan. During three years of birds population assessment highest count were 57,892 birds belonging to 188 species for 2011-2012, 54,311 birds of 186 species in 2012-2013 and 52,682 birds of 187 species in 2013-2014.. A slight decreasing trend in bird population was observed during three years. The different direct or indirect threats were observed at the site. It was noticed at site that a family of 6 person used 120 kg of wood per month and in this way they burnt 1440 kg of wood annually and promote deforestation. Hunting of birds and other fauna was remarkable at site, total reported cases of illegal hunting were 539 in three years and hunting index was 14.7. Unsustainable fisheries was common at the site a total of 151 cases of illegal hunting of Mahasher and other fishes were reported by means of katra net, electric current and blast fishing. Uprooting of woody trees and livestock grazing at shoreline and buffer was also noticed at the site. Other threats included water pollution, poverty, illiteracy, poor wildlife protection law enforcement and community hatred. In the light of above facts a sustainable management plan has been proposed to conserve and protect the natural resources of the Mangla dam.