آہ حبر امت!
الی اﷲ اشکولاالی الناس اننی
اری الارض تبقی والا خلاء تذھب
وادریغا!جو خامۂ گوہر فشاں چالیس برس تک اسلامی علوم وفنون کے انمول موتی صفحۂ قرطاس پربکھیرتا اورلٹاتارہا۔گذشتہ ماہ جون کی ایک صبح کو یک بیک خاموش ہوگیا۔وہ مسیحا نفس جواپنے انفاس قدس سے اسلامی احساس وفکر کے تن بے جان کی عروقِ مردہ میں زندگی کانیا اور تازہ خون دوڑاتا رہا۔دین قیم کاوہ پیکر خجستہ گوہر جو اپنے لب ِاعجازنما سے قال اﷲ اورقال الرسول کاپیام حق التیام ایک عرصہ تک جھوم جھوم کے سناتارہا۔علم وفضل،عمل وکردار اوراخلاق وشمائل کاوہ پیکر حسین جو اس عہد میں اسلام کی چہاردہ صد سالہ تاریخ کی آبروتھا اورجس کانفس نفس گلبن دین محمدی کی عطر آفرینیوں کاامین و رازداں تھا۔اچانک خاکِ لحدکی امانت بن گیا۔ملت بیضا کی ایک متاع گراں مایہ لٹ گئی۔بزم انس وقدس کا چراغ فروزاں بجھ گیا۔یعنی حبر امت مولانا سید مناظر احسن گیلانی نے اس عالم آب وگل کوخیرآباد کہہ کر عالم آخرت کی راہ لی۔اناﷲ وانا الیہ راجعون
مولانا ضلع مونگیر(بہار)کے ایک گاؤں گیلانی کے خاندان ِسادات کے چشم وچراغ تھے۔منطق اور فلسفہ کی تکمیل مولانا ابوالبرکات ]کذا: برکات احمد[ ٹونکی سے کرنے کے بعد دارالعلوم دیوبند پہنچے اورشیخ الہند مولانا محمود حسنؒ سے درس حدیث لیا۔اپنی ذہانت وذکاوت،استعداد علمی اورصلاح ونکوکاری کے باعث دارالعلوم کے عمائد میں اتنا رسوخ پید اکرلیاتھا کہ فراغت کے بعد وہیں معین المدرسین ہوگئے۔اس زمانہ میں دارالعلوم کاماہنامہ’ القاسم‘ بڑی آب وتاب سے نکلتاتھا، اس کی ادارت کی خدمت بھی آپ کے سپرد ہوگئی۔اس دور میں آپ نے جومضامین لکھے وہ خود بتا رہے تھے کہ یہ ستارہ ایک دن آفتاب بننے والاہے۔یہاں ماہوار مشاہرہ بطور وظیفہ تیس روپے ملتاتھا۔بہت چاہا کہ کسی طرح پچاس روپے ہوجائے توپوری زندگی ہی مدرسہ کی خدمت کے لیے وقف کردیں۔ لیکن قدرت کو توبہت بڑا اور اہم...
Abstract The aim of this research was to examine the impact of post-COVID stress on the anxiety and coping mechanisms of healthcare workers. A phenomenological approach was utilized to gain a deeper understanding of the personal experiences of the participants. The results of the study revealed the challenges faced by healthcare workers in the aftermath of the pandemic and the coping strategies they employed to manage stress. These findings provide valuable insights for healthcare organizations and policymakers in their efforts to support the mental well-being of healthcare workers. Keywords: Post-covid-19, anxiety, depression, infection, mental health, pandemics, frontline workers, qualitative study, covid aftermaths
Hepatitis C virus (HCV) is one the major threat to public health being leading cause of chronic liver disease with 92 to 149 million people affected worldwide. A rapid and accurate diagnosis is the key to prevent viral transmission and management of disease progression. A point-of-care (POC) system was designed for rapid detection of HCV infection called as STALLION (Single Tube Analysis using LAMP, LED and ION-sensing).Viral RNA was initially isolated using conventional spin-column and magnetic bead based methods, however, for POC setup, two modified methods were described. The first method uses a millifluidic channel with nucleic-acid-binding-silica-membrane made with the help of a novel approach called grid sandwich method (GSM).This method uses plastic canvas grid to make fluidic channels and then canvas grid is sandwiched between two sheets of polymeth-methacrylate (PMMA) sheets and fixed with the help of suitable adhesive. The second method employs transparent soft tube with reagents separated by surface tension valves and isolation is performed by nucleic-acid-bindingmagnetic-beads. The later approach was used with the currently designed STALLION system for isolation of RNA. A custom detection probe was made to dip into PCR tube comprising an ion sensitive field effect transistor (ISFET), a micro-capillary based Ag/AgCl reference electrode and a temperature sensor. In-vitro replication of HCV genomic RNA was performed by reverse transcription loop-mediated isothermal amplification (RT-LAMP) and detected in real-time by ISFET bio-sensing of released H+ ions. Heat incubation at 60°C was provided by a very low cost novel concept using dissipated heat from power LED. With this system, the HCV positive samples with 101, 102, 103 copies/ml were detected in as less as 30, 18 and 13 minutes respectively against no-template control (NTC). Detection limit was comparable to available methods such as nested PCR. No significant change was observed for HCV negative samples.The results can be viewed on associated LCD screen or alternatively can be transferred to computer or smart phone. STALLION system provides analysis in conventional PCR tube format avoiding any complex fluidics or instrumentation requirements. Such a system will be particularly useful for POC, rapid and reliable clinical diagnosis of HCV RNA with no prior expertise in limited resource settings especially for developing countries.Further, STALLION system was tested for detection Malarial parasite which is a DNA containing infectious agent. The testing was also performed using fluorescent dye and custom designed real-time detection setup. These experiments showed that STALLION system can be effectively used for the detection of any organism whose nucleic acid can be amplified with LAMP in POC settings making it suitable and convenient for testing especially in remote and under-developed areas. In conclusion, the STALLION setup provides a complete system to perform RT-LAMP (from RNA source) or LAMP (from DNA source), and easy scalability for performing other isothermal nucleic acid amplification tests using specific primer sets and standardization accordingly.