جوا اور اس کے انسان کی معاشی اور معاشرتی زندگی پر پڑنے والے اثرات اور اسلامی تعلیمات کا مطالعہ

Among the prohibited actions, gambling is on the top. There is definite prohibition  in many verses of the Holy Quran and the hadiths of the prophet. Despite such strict prohibitions by Islam, the gambling has been entered in economy in different shapes. Among the different types of gambling, some  forms are  very  clear, every one considers it wrong,   whereas  some kinds of  gambling are not considered wrong by the peoples. Whatever the name of gambling is, it is illegal and immoral, because changing name doesnot change its reality. Every type of gambling creates destruction in society and economy and brings various evils with it. Gambling  makes man greedy, miser, lover of  money, jealousy, wastage  of time and money. Such evils takes men away from generosity and  worship of Allah, carelesness in his duties and man becomes  immoral and irrespossible. Therefore, in any society where gambling is spread, the concept of peace, love, humanity bacomes  meaning less. As a result, the poor get poorer and the  rich grow richer.

Cloning and Insilico Characterization of Itr1 Gene from Bread Wheat Advance Lines

Iron being an important micronutrient plays a vital role in maintaining life on planet earth. It is equally important for plants and animals. Deficiency of iron is one of the major causes of anemia. The present study was performed with the objective of gene cloning and the in-silico characterization of metal transporters in wheat.Experiments were conducted on the wheat advance lines varieties ML 16 and GF 16, for which seeds were obtained from Wheat Research Institute, Ayub Agriculture Research Institute, Faisalabad. Seeds were grown in growth chamber androots of plant were used for RNA extraction. The IRT1 iron transporter from wheat (Triticum aestivum L.) advance lines was cloned and in silico characterized at Plant Molecular Genetics and Genomics Laboratory in department of Botany GC University Faisalabad. The IRT1 was cloned and sequenced for in silico characterization and compared with other iron transporter sequences. It is revealed that IRT1 gene is present in the cereal crop like maize, rice and wheat and molecular study revealed that the size of protein is 2532.0 Da. Multiple sequence alignment (MSA) of the cloned gene exhibited 100% similarity with iron transporter gene of Oryza sativa. The MSA also clearly indicated a conserve region of ZIP gene family. The present study can be used as a mile stone for understanding of iron transporters in wheat under different set of climatic conditions and wheat breeding programs for introducing new wheat varieties with better iron contents in future.