نشرا لطیب فی ذکرالنبی الحبیب ﷺ کاتعارف و منہج؛ ایک تجزیاتی مطالعہ An Analytical Study of the

Sira of the Holy Prophet ﷺ is the only subject that can quench the thirst of every subject in the universe and guarantee the solution of every problem. Love and Obedience to the Holy Prophet (peace and blessings of Allah be upon him) is a must. Mawlana Ashraf Ali Thanwi ranks high in the list of biographers through his famous book "Nashr al-Tīb" in remembrance of the Beloved Prophet (May God bless him and his family and grant them peace) which is comprehensive enough to do its function. The book is one of his excellent works in its own way. It is a valuable book in Urdu language which has been presented in an unparalleled manner. It shows the love of the Holy Prophet ﷺ as well as his teachings; mysteries and secrets which is Narrated in the style of Mathnavi Maulana Rum. In the said article, the introduction and method of " Nashr al-Tīb fi Zikr-un-Nabi Al-Habib" will be presented. In order to know the services and style of writing and purpose of Mawlana in Sira Nigari and the only purpose of Sira Nigari is to achieve love of of the Holy Prophet and obedience to Him ﷺ.

Association Mapping of Fiber Quality Traits in the Genome of Genus Gossypium

Harvesting high quality lint, a long-awaited breeding goal—accomplished partly, can be achieved by identifying DNA markers which could be used for diagnosing cotton plants containing the desired traits. In the present studies, a total of 185 cotton genotypes exhibiting diversity for lint traits were selected from a set of 546 genotypes evaluated for fiber traits in 2009. These genotypes were extensively studied for three consecutive years (2011-2013) at three different locations. Significant genetic variations were found for average boll weight, ginning out turn (GOT), micronaire value, staple length, fiber bundle strength and uniformity index. IR-NIBGE-3701 showed maximum ginning out turn (41.10%). Clustering of genotypes using Ward’s method was found more informative than that of the clusters generated by principal component analysis. A total of 382 SSRs were surveyed on 10 G. hirsutum genotypes exhibiting contrasting fiber traits. Out of these, 95 polymorphic SSR primer pairs were then surveyed on 185 genotypes. The gene diversity averaged 0.191 and the polymorphic information content (PIC) averaged 0.175. Unweighted pair group method with arithmetic mean (UPGMA), principal coordinate analysis (PCoA) and STRUCTURE software grouped these genotypes into four major clusters each. Genetic distance within the clusters ranged from 0.0587 to 0.1030. A total of 47 (25.41%) genotypes exhibited shared ancestry. In total 6.8% (r2 ≥ 0.05) and 4.4% (r2 ≥ 0.1) of the marker pairs showed significant linkage disequilibrium (LD). A number of marker-trait associations (in total 75) including 13 for average boll weight, 18 for GOT percentage, eight for micronaire value, 18 for staple length, three for fiber bundle strength and 15 for uniformity index were calculated. Out of these, MGHES-51 was associated with all the traits. Most of the marker-trait associations were novel while few validated the associations reported in the previous studies. High frequency of favourable alleles in cultivated varieties is possibly due to fixation of desirable alleles by domestication. These favourable alleles can be used in marker assisted breeding or for gene cloning using next generation sequencing tools. The present studies would set a stage for harvesting high quality lint without compromising the yield potential—ascertaining natural fiber security.