بولیاں
پنجابی لوک گیتاں دی اک قسم بولیاں اے بولیاں جمع دا صیغہ اے۔ جیہدا واحد بولی اے تے جیدے کئی معنی نیں بولی دا لفظ زبان دے معنیاں وچ ورتیاں جاندا اے بولی پنجابی شاعری دی اوس قسم نوں کہندے نیں جیہدا صرف اکو مصرعہ ہوندا اے بولی اک مصرعہ ہون پاروں ردیف قافیہ نئیں ہوندا بعض کتاباں وچ بولی نوں دو مصرعے وچ ہیٹھ اُپر لکھیا جاندا اے انج لکھن وچ ہرج تے کوئی نئیں پر ویکھن والیاں نوں بھلیکھا پیندا اے کہ بولی دو مصرعیاں نال بن دی اے بھانویں اوہناں دویاں مصرعیاں وچ ردیف قافیہ نئیں ورتیا ہوندا۔
ذرا ساہ لین نوں روک کے پڑھن نوں پنجابی وچ ورام آکھدے نیں چھوٹے مصرعیاں وچ ورام دی لوڑ نئیں پیندی پروڈے تے لمبے مصرعیاں وچ دو، دو رام وی آجاندے نیں ایس لئی بولی دے مصرعے نوں جیہڑا لماں وی ہوندا ایں اک ورام نال پڑھدے نیں جہناں نے ایس بولی نوں دو مصرعیاں وچ بنا دتا ایں اوہناں نے ورام نوں صحیح نہیں ورتیا۔
ایہہ مثال ویکھو۔ بولی اے:
Youth is the real asset of any nation as it plays an important and significant role in the development of society. Youngsters are the future of any nation as they take the charge of the promotion and progress of the society. If youth of a nation stands corrupted the entire nation slides into regression and decadence. But if the youth of a society treads the right path the entire nation succeeds both here and hereafter. Adolescence is a time when a person undergoes changes both physically intellectually. This study was basically designed to explore the role of young companions of Holy Prophet (ﷺ) in the defense and development of Islamic state Madīnah. The approach applied for the collection and analysis of data was qualitative and descriptive. The review of literature uncovered that the young companions, progress the in role cardinal a played (صلى الله عليه وسلم) Muhammad Prophet of advancement, defense, and development of the society. The Holy Prophet young of education and training the towards attention special paid (صلى الله عليه وسلم) companion which enhanced their capacity and level of motivation and led them to work with enthusiasm. The kind behavior of our Prophet Muhammad (ﷺ) enabled them to transform their selves, their families, Meccah, Madīnah and the surroundings of the these sacred cities. Within the shortest possible period they spread the peaceful message of Islam and brought a positive change in the society and particularly established the peace in Madīnah. They built a strong defense of Madīnah from internal and external challenges and laid strong foundations for social, moral, economic and political development of the Islamic state Madīnah. It is, therefore, recommended to encourage the youth of Pakistan to make them realize their duties towards Pakistan and Islam so that they can play a significant role in the development and progress of Islam and Pakistan.
Hepatitis C was called ?vigorous time explode? because it can extend rapidly and silently and
disturbing more than 200 million people all over the world every year. According to some
recent researches, in Pakistan, almost 10 million peoples were affected by HCV. The
occurrence of hepatitis differs from country to country and varies among discrete areas of the
same country. The major cause of increased HCV prevalence among the Pakistani was the
reuse of injected syringes, unrestricted blood transfusions, and attentiveness. Activation of
these TLRs may contribute to the inflammatory destruction caused by HCV. It had been
shown recently that, HCV targets the TLR3 pathway to aid in immune evasion. TLR3 was
only one of four viral recognition receptors placed within the endosome and it was plausible
that HCV may target others. The aim of this research is to seek out the causes of HCC
development in HCV patients of Pakistan.
To pursue this research study, 200 blood samples of HCV patients were collected from the
DHQ hospital of Okara city. Then blood samples of HCV were undergone through the DNA
extraction process, which was the initial step and most basic step for this analysis, later Gel
Electrophoresis was completed form size and excellence of extracted DNA. After that,
specific primers of TLR3 gene was designed according to target areas and then optimized with
reaction mixture to get copies of targeted genes, During PCR amplification, RTPCR
conditions were applied to find the specific annealing temperature which gives the maximum
amplification of target region and use the minimum reaction mixture to get the desired results.
After DNA amplification through PCR, another technique named as a High-resolution
melting curve was used to find the percentage of mutational occurrence is controlled and
HCV patient, which leads to identifying the progression of disease in individuals. By applying
the HRM method, some values were extracted to estimate the chance of occurrence of
heterozygous condition TC for the TLR3 gene in both HCV and control patients which are
0.039 and for homozygous condition CC was 0.017. There was a significant difference
between the dominant and recessive models as 4 (4%) of control and 11 (6%) of HCV patients
shows chi-square value for TC+TT as 0.86(0.57-1.29) and p-value as 0.538. On the other
hand, the recessive model gave a value of 8.24 for 57(57%) control and 43(43%) HCV
patients.We assumed that HCV infection may intrude with the appearance and role of TLR3, a sensor
of ssRNA. Investigating any effect on TLR3 by HCV may disclose a new mechanism for
HCV immune evasion. In conclusion, the Hepatitis C Virus manipulates specific TLR3
function and expression and their signaling pathways disturb cytokine production. HCV
modifies TLR3, a viral recognition receptor, via decreasing mRNA constancy which could
enable the illusion of host immune observation. There was an association between the
occurrence of HCC and infection with HCV in Pakistan.