اسم ِ استفہامیہ :أنّٰی کہاں؟
ارشادِ ربانی ہے:
"اَنّٰى لَهُمُ الذِّكْرٰى وَقَدْ جَاءَهُمْ رَسُوْلٌ مُّبِيْنٌ"۔ [[1]]
"ان کے لئے نصیحت کہاں ہے؟ کھول کھول کر بیان کرنے والے پیغمبر ان کے پاس آچکے"۔
رسولِ مُبین کے دو مطلب ہیں ۔
ایک یہ کہ اس کا رسول ہونا اس کی سیرت، اس کے اخلاق و کردار اور اس کے کارناموں سے عیاں ہے۔
دوسرا یہ کہ اس نے حقیقت کو کھول، کھول کر بیان کرنے میں کوئی کسر نہیں اٹھا رکھی ہے۔اُس وقت یہ ماننے کا کوئی فائدہ نہیں : سو ارشاد فرمایا گیا کہ " اس وقت ان کے لیے نصیحت کا کوئی موقع کہاں باقی رہا جبکہ اس سے پہلے آچکے انکے پاس کھول کر بیان کرنے والے ایک عظیم الشان رسول " ۔ ایسے عظیم الشان رسول جن کی صداقت و حقانیت روز روشن کی طرح واضح تھی۔ اور واضح ہے۔ مگر پھر بھی یہ لوگ ایمان نہیں لائے تو اس کے بعد اب کیسے اور کیا ایمان لائیں گے؟ سو اس وقت ان کی تذلیل و تخجیل کیلئے اللہ کی طرف سے انکو یہ جواب دیا جائے گا۔ بہرکیف ارشاد فرمایا گیا کہ اِعلانِ حق کے پہنچ جانے اور اس کے دیکھ لینے کے بعد ایمان لانے اور نصیحت قبول کرنے کا موقع کہاں باقی رہے گا۔ بالخصوص جبکہ انکے پاس اِتمامِ حجت کیلئے اللہ تعالیٰ کی طرف سے ایک ایسا عظیم الشان رسول بھی پہنچ گیا جس نے انکے سامنے حق کو پوری طرح واضح کرکے اور نکھار کر بیان کر دیا تھا۔ لیکن انہوں نے اس کی بات کو مان کر نہ دیا سو ایمان لانے کا وہ موقع جب گزر گیا تو...
The experts from various fields have maintained some rules and regulations in understanding and acquiring skills in this field of knowledge. This is due to their efforts which make very simple to understand it similarly the experts of ʽilm-ul-Fiqah i.e. Mujtahedyn and respected Fuqahāʼ have formulated some rules and regulations in the light of Qurʼan, Sunnah and decision act of companions of Muhammad (SAW) which also made it not only simple but it furnish skills and strategies in solving problems in field of Fiqah.
Germin and germin-like proteins (GLPs) constitute a large and highly diverse family of developmentally regulated proteins showing a wide range of distribution from Myxomycetes to flowering plants. These proteins have been cited in various plant organs like roots, leaves, nectar glands and seeds etc. Germin-like proteins also seem to be involved in many stress related processes, but their definite role in these processes remains obscure. The present study was conducted to elucidate the function of germin-like protein gene (OsRGLP1) of rice by over-expressing OsRGLP1 cDNA under the transcriptional control of strong promoter in tobacco. The cDNA of OsRGLP1, 958 bp was successfully cloned in pTZ57R/T cloning vector. OsRGLP1 cDNA was inserted in p1301 in sense direction down stream of CaMV35S promoter and transformed into Agrobacterium strain EHA101. Transgenic tobacco plants were produced through Agrobacterium mediated transformation and confirmed through hygromycin resistance and PCR amplification of OsRGLP1. Transgenic plants were assessed for growth, morphology, oxalate oxidase and superoxide dismutase activities. Morphologically no difference was observed between T0 transgenic and control plants except that leaves of some mature transgenic plants showed necrotic lesions. Transgenic plants were evaluated for the presence of H2O2 which is reported to be involved in necrosis and cell death. Interestingly, higher levels of H2O2 were observed in transgenic plants. Seeds of T0 /T1 transgenic plants were similar in shape, size and coat color to the control plants when harvested, whereas, seed coat of transgenic seeds turned lighter in color after one year storage at room temperature. Germination rate and percentage in transgenic T1 seeds was significantly lower than control seeds. Segregation analysis indicated a 1:1 ratio for hygromycin phosphotransferase gene in T1 seeds. There was no difference in fresh/dry weights, plant heights and shoot lengths, number of leaves and nodes of transgenic and control plants. Number of roots in transgenic plants was lower while roots were longer than control plant roots. No oxalate oxidase activity either of native or transgene origin could be observed in transgenic plants revealing that OsRGLP1 may not posses this activity. Significantly higher levels of heat resistant superoxide dismutase activity were observed in transgenic plants as compared with control. This added activity was insensitive to KCN and sensitive to H2O2 suggesting being due to FeSOD activity. Present study indicates for the first time that OsRGLP1 may posses SOD activity along with a striking observation that this activity is FeSOD type instead of MnSOD, if at all observed. Upstream regulatory region of OsRGLP1 was also cloned, sequenced and analyzed to gain insight into the regulatory aspects. The promoter region contained many regulatory elements related to diverse functions, most intriguing were the elements related to biotic and abiotic stress which emphasizes the possible involvement of OsRGLP1 in these processes. These findings may suggest that OsRGLP1 gene product may act as FeSOD responsible for dismutation of stress generated reactive oxygen species into H2O2 which in turn may play important roles like defense against pathogen and root elongation.