Contemporary Debate on Peace, Politics and Religion: A Quranic Perspective

In the contemporary era of conspiracy theories and practices through media prejudice, focused scholarship and policy oriented publication, Islam in general and Muslims in specific are being tinted as anti-peace and social prosperity entities. Quran as the primary source of Islamic jurisprudence provides principals for every aspect of society including polity one. This study focuses on basic Quranic injections regarding politics and their role in developing peace in contemporary society. The compatibility of Islam and democracy is one of the hottest debates among researchers of political science are also to be focusedon the study. Applyinghermeneuticsmethod and analyzing thought of key Muslim political thinkers and interpreters, this paper concludes that Quranic injections of polity and state are a vibrant source of developing peace and prosperity in historical perspective and same applicable in contemporary society, but hegemony forces feeling fear of Islamic resurgence state that Islam and democracy are incompatible and these Quranic sources are being used for creating panic in the present world.

Mutagenesis for Hyper Production of Streptokinase from Streptococcus Equisimilis and Comparative Sequence Analysis of Sk Gene

Streptokinase (SK) is protein excreted by strains of β-hemolytic Streptococci. It is applied as treatment of life-threatening diseases thrombosed arteriovenous shunts, and thromboembolism. Streptokinase is naturally produced by several bacterial strains especially Streptococci. In connection with its broad application in the field of medicine, there is an urgent need to search for methods of its hyper production. Present study aimed at SK hyper production by mutagenesis of S. equisimilis strain. The wild bacterial strain S. equisimilis was subjected to random mutagenesis (UV irradiation and Ethidium bromide) for enhanced streptokinase production. The modified strain was assessed for the activity of streptokinase. The mutant S. equisimilis UV6 showed the maximum SK production and activity. By standardizing the effects of different factors like substrate (CSL), carbon source, salt concentration, temperature and pH an optimum fermentation protocol for SK production was established. The higher SK production was observed in mutant strain UV6 601 U mL-1 with increased activity up to 400 %. The SK was purified and its kinetics data indicated improved activity as its Km value was low as compared to wild SK. The SK gene from mutant UV6 was amplified and cloned in E. coli using pTZ57R/T vector. The gene was sequenced and compared with the wild SK gene to check the genetic difference between wild and mutant strain. In our study, the comparative sequence analysis revealed 26 point mutations, among these 3 were transitions (A to G) while the remaining 23 were transversions (A/G to C/T) in coding region of sk gene. Moreover, 3 amino acid deletions at the C terminal region of SK protein were predicted due to non-sense mutation in coding region (resulting in a stop codon after residue 437). The activity of any protein is direcly linked with amino acid composision. in our studies, the in-silico analyses predicted that amino acid residues at position 22, 62, 64 and 80 could be accountable in SK hyperactivity.