Implementation of Character Education: Impacts on Students and School Components at Madrasah Aliyah Negeri (Man) Insan Cendekia Gorontalo

This study aims to determine the implementation of special character education on the impact for students and school components at Madrasah Aliyah Negeri (MAN) Insan Cendekia Gorontalo. This research method is a qualitative field research. Data collection was carried out through preliminary or pre-observational studies, observations, and interviews. The characters developed in this madrasa include disciplined, religious, polite, responsible, hard work and cooperation. In other words, this character development is carried out in the madrasah program of activities both through learning, as well as extra ¬ curricular as well as religious and self-development activities. The impact of the results of the implementation of character education at MAN Insan Cendekia Gorontalo has a positive impact for all groups, especially for students and the school community. The impact of character education for school components. The implementation of character education at MAN Insan Cendekia Gorontalo is very beneficial for students, due to changes in attitudes that are even better and according to the expectations of parents, where their children live disciplined, productive, independent and able to practice religious teachings.

Engineering of Microbial Fungal Glucoamylases by Chemical Modification Techniques for Industrial Application

Glucoamylase (exo-1,4-α-D-glucan glucanohydrolase: EC 3.2.1.3) yields ß-D- glucose from the non-reducing chain ends of a wide range of polysaccharide substrates and play a tremendous role in the improvement of starch based food products. Non-traditional use of glucoamylases in detergent industry has a great scope of expansion the world over in the coming days. Industrial employment of glucoamylase demands its hardiness to harsh operational environment. Thermo-stable enzymes can withstand extreme environmental conditions and can resist against detergents, organic solvents, temperature & pH. The operational stability of enzymes is of paramount importance for any bioprocess, which can be improved through various protein engineering techniques. With the development of novel procedures that exploit selective and efficient protein chemistry, chemical modification, either alone or combination with other mutagenesis techniques, could make a significant contribution to the development of enzymes that cope with the industrial demands. Various fungal strains (A. niger, A. oryzae, A. tamarii, A. fumigatus, A. flavus and Humicola sp) were grown in submerged cultivation at different temperatures, pH and substrate concentrations in order to optimize GA production. A. niger and Humicola sp were selected for their higher GA production ability as compared to other fungi. The apparent molecular mass of Humicola sp and A. niger GAs determined through SDS-PAGE were 72.8 and 93 kDa. A. niger GA was proved to be much better as compared to Humicola sp GA on the basis of, wider pH range, higher optimum temperature, more turn over (kcat) and higher specificity constant. It also showed 10 fold higher resistance against thermal unfolding and an activation trend against α- chymotrypsin A. niger was decided to be improved through γ-rays treatment with the main objective to enhance the production, stability and function of the enzyme. The 1 kGy γ-rays exposed mutant (M-7) of A. niger was selected on the basis of deoxy-D- glucose resistance. The mutant M-7 was about two fold efficient for extracellular GA production in submerged cultivation on Vogel’s medium containing 4% (w/v) wheat bran, pH 5.0 at 30 oC. The mutant GA had same molecular mass on SDS-PAGE, however on MALDI-TOF a difference of about 814 Da was observed. The mutant GA proved to