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Development and Testing of Composite Material from Waste Liquid Crystal Displays

Thesis Info

Author

Malik Daniyal Zaheer

Supervisor

Saeed Badshah

Program

MS

Institute Type

Public

City

Islamabad

Country

Pakistan

Degree End Year

2020

Thesis Completion Status

Completed

Subject

Mechanical Engineering

Language

English

Added

2021-02-17 19:49:13

Modified

2023-01-06 19:20:37

ARI ID

1676729609117

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خلاصہ بحث

فقہی اور قانونی فکر کے حوالے سےمسلم تمدن جس انتشار کا شکار ہے اس کے سدباب کے لیے اہل علم کو فرو عی مسائل میں الجھنے کی بجائے مسالک کو قریب کرنے کے لیے کام کرنا چاہیے۔

ڈاکٹر غزالی ‘‘احکام القرآن’’ کے بارے میں کہتے ہیں کہ حنفی فقہی روایت کی شان و شوکت یہ تفسیر ہے۔ البتہ یہاں ایک اہم سوال ہے کہ احکام قرآن پر جتنی بھی تفاسیر لکھی گئیں۔ ان کا بڑا ہدف اور مقصد اپنے مسلک کی نگہبانی کرنا ہے اسی وجہ سے فقہی تفسیر اپنی علمیت اور موضوعیت کھو بیٹھی۔ دراصل مولانا اشرف علی تھانوی کی یہ تفسیر عالم اسلام کے اطراف سے اٹھنے والی ایک مخلص صدا کا ایک سلسلہ ہے۔ جس سے مراد یہ ہے کہ اسلامی فقہی مسالک و مذاہب سے بیزاری مسائل کا حل نہیں اوران لوگوں کے لیے لمحہ فکریہ ہے جو فقہی مدارس کو فرقہ واریت کا سبب قرار دیتے ہیں۔ ان کو اس ملک سے نکال دینا چاہیے کیونکہ امت کے مرض کی تشخیص اور اس کے علاج کی تجویز میں ہر دور میں غلطی ہوئی ہے ۔

Post-Covid 19, Online consumer purchase behavior in Pakistan

Since Covid-19, there has been a rise in e-commerce and online brands' influence on consumer buying behavior. It is crucial to investigate the factors that impact online consumer purchase behavior. Applying the theory of reasoned action (TRA), our study seeks to assess the extent to which customer satisfaction and perceived risks build trust and how trust, consumer engagement, and ease of purchase would act as reasons for consumers to engage in e-commerce in Pakistan. Using data gathered from 400 respondents via a questionnaire, we have demonstrated the precise relationship between these six constructs. Results indicate that customer satisfaction positively influences trust while perceived risks negatively affect it and that trust and consumer engagement positively and directly impact purchase behavior. Online retailers should improve these factors and modify the online environment to increase sales from their e-channels and improve customer retention

Dna Fingerprinting and Characterization of Mutations Associated With First Line Anti- Tb Drug Resistance in Mycobacterium Tuberculosis Strains Prevalent in Pakistan

Tuberculosis (TB) is one of the most devastating infectious diseases that is highly endemic in Pakistan. Pakistan is ranked 5th amongst 22 high tuberculosis burden countries of the world. Global as well as national tuberculosis control program is further challenged by the spread of multiple drug resistant strains of Mycobacterium tuberculosis, the causative agent of tuberculosis. For controlling the spread of M. tuberculosis isolates, circulating in this region, it is important to explore the transmission dynamics and characteristics of these strains. This information, in turn, can help to implement better treatment and control measures. The study provides the information about the population structure of M. tuberculosis isolates in Pakistan. DNA fingerprinting of the strains was performed by high throughput spoligoriftyping and 24 MIRU-VNTR typing techniques. CAS family constituted the dominant group of the strains followed by the T family and EAI family while Beijing family showed the low prevalence. However, EAI strains were found to show high prevalence in Eastern part of the country. Molecular epidemiological methods play an important role to identify appropriate public health interventions and to measure their impact. Despite of the fact that Pakistan is harboring high disease burden, no molecular epidemiologic studies have yet been conducted to assess the disease transmission. Our study explores, for the first time, TB epidemiology in the Punjab province of Pakistan, using the gold standard tools of molecular epidemiology. We document a relatively low disease transmission rate in the population. The study also provides a good assessment of the discriminatory powers of the various genotyping techniques and suggests the use of duplex format of MIRU-VNTR typing to be used as genotyping technique in this setting because of its low cost and relatively less turnaround time as compared to simplex format. We further suggest the use of Qub 26, MIRU 10, Mtub 04, MIRU 26, MIRU 31 (ETR E), MIRU 16, Qub 4156 and Mtub 21 to be used as preliminary ‘fast lane’ screen to differentiate the M. tuberculosis strains in this particular geographical setting. The use of high throughput techniques like spoligoriftyping is recommended to be used as good tool to help the TB control programs in high disease burden countries. This powerful technique also helped to identify unreliable standards of phenotypic drug sencitivity testing (DST) in some local hospitals. Besides this, an in-house low cost test platform is configured and validated for the rapid screening of multiple drug resistance (MDR) in the present study. Being highly sensitive and specific, not only in the culture isolates but also in clinical samples, it could be used to screen MDR in point of care settings in the developing world where the need is acute. This study for the first time gives the comparative assessment of three freely available databases used to assign lineage to the M. tuberculosis isolates, uncovering the errors and inability of these databases in assigning lineages to isolates. Further, our study also pinpointed the defects in lineage assignation at sublineage level that arose due to the lack of database up gradation. The study also covered the assessment of occurrence of mutations at various target loci and their frequencies in M. tuberculosis isolates, resistant to first line anti- TB drugs. Overall, the profile of the mutations at various loci was similar to that found at other geographical locations worldwide. The most common mutations responsible for the rifampicin resistance were found in codon 531, 526 and 516 of rpoB gene, in isoniazid resistant isolates affecting the codon 315 of the katG and position -15 of the promoter region of inhA gene, in ethambutol resistant isolates affecting the codon 306 of embB gene and in streptomycin resistant isolates, targeting the codon 43 of rpsL gene and codon 512, 513 and 516 of rrs gene. In case of pyrazinamide, very few isolates showed mutations in targeted regions of pncA gene. Besides this, some novel mutations were also observed in this study. The relationship of specific mutations in rpoB and katG genes with M. tuberculosis lineages is also explored. The information about these mutations can be used to develop novel molecular diagnostic method that specifically could be implemented in Pakistan. However, prospective thorough epidemiological studies are needed to monitor continuously changing disease transmission dynamics in the community.