The Investigative System of Islam Investigative System of Islam

The Holy Prophet (PBUH) was sent to establish justice. He made use of law and good conduct to achieve this great target. Complete investigation and transport exploration is of universal value that holds fundamental significance in legal system. This research article presents insight, broadness and transparency of investigatory system established by the Prophet (PBUH). It also highlights rules and laws in the light of valid research references. All these features are evident in Prophet’s personality. In this regard, keeping in view the nature of topic, the guidance has been sought from the commands of The Quran, Hadith and different incidents of the life of Muhammad (SAWW). A discussion has been premeditated to explore Prophet’s tactics regarding law of investigation. This research article, having research outlook and logical link, touches upon the rules and principles which the Holy Prophet (PBUH) has given us for the recognition of the real culprit of good judgment, clues and witnesses. Investigative departments are radically important for the protection of Islam, maintenance of peace, internal stability and the protection of life, honor and property of people living in the society. The charter of these agencies is to abolish oppression, establish justice, impose the writ of law, and punish the guilty and to bring lawbreakers within the grip of law. The officer who performs the above duties is known as Naazir e Jaraaim1 in Islamic jurisprudential terminology. Further duties which fall within the remit of this officer are: Investigation of charges, production of the accused before a court of law, getting proven criminals sentenced by the court, executing the sentence handed down, releasing   the accused in case evidence is not sufficient and getting wrongful accusers punished properly.

Investigation of Active Site of Osrglpi. a Germin-Like Superoxide Dismuatse

Germin-like proteins are glycosylated proteins that are highly diverse in plant kingdom. Most of the germin-like proteins show superoxide dismutase activity. Superoxide dismutase converts superoxide anion into molecular oxygen and hydrogen peroxide. Identification of catalytic residues is an important step for understanding the mechanism of enzyme catalyzed reactions. Previously OsRGLP1 gene and cDNA were isolated from the roots of Oryza sativa and, under CaMV35S promoter, cDNA has been heterologously expressed in tobacco. In transgenic tobacco plants, OsRGLP1 seems to show SOD activity. Present study was aimed to determine the active site of OsRGLP1. Three dimensional structure and residues involved in metal ion and substrate binding were predicted using bioinformatics tools. Three histidines and one glutamate were predicted to play an important role in metal ion binding and Asparagine to Alanine mutation was predicted to be responsible for different substrate specificity of germin and germin-like proteins. These residues were then mutated with other selected residues by site directed mutagenesis to confirm these predictions. In present study out of many designed mutants, four loss of function mutants were further studied by performing transient transformation of tobacco plants. These transgenic plants were used as source of native and mutant proteins for SOD activity assay to find the effect of mutations on SOD activity. Results of SOD assay showed approximately complete loss of activity in all mutant proteins validating the importance of these residues in SOD activity of OsRGLP1 protein. As these residues are involved in metal ion binding so present study also provide some insight into mechanism of action of OsRGLP1 indicating OsRGLP1 indicating the role of metal ion in its activity.