ہو ملاقات جو اپنوں سے یا اغیار کے ساتھ
کتنا اچھا ہو اگر سب سے ملیں پیار کے ساتھ
تو نے منصور بڑی دار کو عزت بخشی
ذکر ہوتا ہے تو ہوتا ہے ترا دار کے ساتھ
میں تو بس دُور ہی رہتا ہوں ہمیشہ ان سے
میری بنتی جو نہیں زر سے نہ زردار کے ساتھ
آسرا حشر میں آقا کی شفاعت ہو گی
کون واں ہو گا بھلا مجھ سے خطاکار کے ساتھ
مجھ کو منظور فقط تیری رفاقت جو ملے
نہ رہے کوئی تعلق مرا سنسار کے ساتھ
ساتھ تیرے بھی وہی ہو گا یقینا تائبؔ
جو کہ ہوتا ہے یہاں ایک وفادار کے ساتھ
The Corona virus (SARS-CoV2) pandemic initiated in late December 2019 in Wuhan city of Hubei, China, which has rapidly progressedinvolving more than 215 countries of the world. It was caused by novel SARS-COV2 coronavirus with Huanan seafood wholesale market as the possible point of origin. In past two decades, coronaviruses epidemic of Middle East Respiratory Syndrome (MERS-COV) had 37% mortality rate and Severe Acute Respiratory Syndrome (SARS-COV) had 10% affecting more than 10,000 population together. World Health Organization (WHO) declared it as the sixth Public Health Emergency of International Concern (PHEIC) on January 30, 2020 and later on March 11, 2020, the WHO labeledit as a pandemic. The first case of COVID-19 from Pakistan was reported on 26th February, 2020 and has affected over 354,000 people with a mortality of over 7000 patients. Many countries of the world have seen second wave of this pandemic. Government ofPakistan has also declared a second waveon October 28, 2020, after the rise in cases from 500 to 750 per day. Now it has crossed 2000 cases. The data released by the National Command and Operation Centre (NCOC) shows that the current percent positivity rate is close to 3 compared to the previous figure of lesser than 2 making it a bigger challenge than first wave in Pakistan. The patients now presenting in hospitals are all in critical condition. Lack of a specific vaccine or antiviral drug and non-compliance to the standard preventive measures is the major reason of initiation of a second wave of this viral infection in Pakistan. Being a nation we need to be responsible. Our country may go into economic crisis & our health facilities may choke. We have to understand how to live with this virus till the availability of vaccine or Curative antiviral drug. TheGovernment of Pakistan is creating awareness in the public for the second wave as the situation of pandemic is getting worse. Smart lock downs are being implementedbut people are not following preventive measures that are leading to infection spread at a very alarming speed. At the moment preventive measures are the only way to stop the spread of disease. Preventive measures should be adopted to contain this deadly disease. Wearing masks, using hand sanitizers, washing hands with soap for 20 sec, keeping social distance of 6 feet are mandatory preventive strategies. Social, political, business, recreationaland religious gatherings, should be avoided. Educational institutesshould follow strict standard operating procedures. Most of the people in Pakistan are not considering this disease as a matter of serious concern due to unawareness, poverty, beliefs and lack of resources. People should ignore such disbeliefs and should start considering it as a great health concern. They should follow the preventive measures in true sense.
Agriculture have been responsible for feeding growing population for decads by developing new technologies of crops especially staple crops like maize, wheat, rice etc. But unfortunately the main focus of agriculture have always been on increasing crop production instead of promoting human health. To improve human health, increase in micronutrient contents in crops are required. To mitigate this micronutrient malnutrition, fortification of food is most common approach. Among fortification, biofortification of crops with essential nutrients provides an economical tool to rescue micronutrient deficiency in target populations globally. The use of plant growth promoting microorganisms (PGPR) is promising technique in this respect. It is well recognized that the mobilization of plant nutrient is promoted by the use of microorganisms and it ultimately reduces the need of chemical fertilizers. However, informations on interventions using PGPR or other biological agents is limited. This study included isolation, screening and characterization of Zn-solubilizing and siderophore producing PGPR and optimization of different compost levels for improving Zn and Fe availability in soils. For this purpose, several zinc solubilizing bacteria (ZSB) were isolated from rhizosphere of wheat crop from different areas and quantified on the basis of zinc solubilizing potential and maximum pH reduction in broth culture by bacterial isolates. The isolates were then checked for their potential to produce siderophores. Af ter that, 35 bacterial isolates were selected and screened on the basis of plant growth promotion activities under axenic conditions. From this, 7 bacterial isolates were selected for pot study. These isolates had ability for IAA production both in presence and absence of L-tryptophane and also had ACC-deaminase activity. These isolates were also capable of producing different types of organic acids that reduces the soil pH. Different levels of compost (0, 200, 400, 600, 800, 1000, 1200, 1400, 1600, 1800 and 2000 kg/ha) was mixed with soil and incubated for different time intervals and then selected on the basis of iron and zinc release pattern through optimization study. With increasing application of compost, the iron and zinc release was also increased and became maximum at 800 kg/ha compost. After that there was non-significant effect of compost on zinc and iron release and with further increase, this release start decreasing. Soil dehydrogenase and phosphatae activities was also increased with compost application. The release was maximum after 45 days of incubation. These compost levels and selected isolates from previous study was then evaluated in pot experiment. Data were analyzed following Factorial under CRD. The inoculation of bacterial isolates showed positive effect on physical, physiological and yield parameters over un-inoculated control. Among the isolates H-103 performed better and showed maximum growth. The results of 800 kg/ha application of compost was significant from lower levels (0 and 600kg/ha) and nonsignificant with higher level (1000 kg/ha). Grain zinc and iron concentration was also significantly increased by combined use of compost and bacterial isolates. These results were then confirmed under field trial. Data were analyzed following Factorial under RCBD. The inoculation of bacterial isolates showed positive effect on physical, physiological and yield parameters over un-inoculated control. Among the isolates H-103 performed better and showed maximum growth. Maximum growth was recorded with 800 kg/ha compost application. Seed zinc and iron concentration was increased and phytic acid contents were decreased upon inoculation. Concluding, the combined use of PGPR and compost is an effective, economical, novel approach for improving yield, quality and nutritional status of wheat crop which can help in combating micronutrient deficiencies. ate showed less height as compared to other isolate, but still it shows a positive response when compared with the control.