Cytotoxic Assessment of Quinoline Based Derivatives on Liver Cancer Cell Line Quinoline compounds and their Cytotoxicity

Current scenario of newer diseases with multiple causes has drawn the attention of the researchers in the field of therapeutics and they are now inclined to identify molecules effective for targeted therapy. Objective: Quinoline (1-azanaphthalene); belongs to heterocyclic aromatic nitrogen compound. Some quinoline-based derivatives are also known for their anti-tumor activity. The study was planned to evaluate the cytotoxic potential of quinoline derivatives. Methods: Berberine; a quinoline compound was made part of study to make structural analogs which were docked against potential target proteins. Cytotoxic profiling of all derivatives was done using MTT cytotoxicity assay. Results: The pharmacoinformatic and structure activity relationship studies of analogs were done. The cytotoxic profiles were elucidated by comparing viability rates of analogs treated hepatic cancerous cell line with untreated hepatic cells and untreated mesenchymal stem cells as standards.  Marked cytotoxicity was seen in all molecules at low doses than reported in past studies with relevance to parent compound. Conclusions: The results will be further confirmed through various other cell culture assays targeting different marker proteins, pharmacoinformatics tools and structure activity relationship studies

Deconstructing Pessimistic Discourse in the Plays of Tennessee Williams

This study uses deconstructive theoretical underpinning to debate the presence of an optimistic discourse contrary to the pessimistic discourse as stressed by the critics and researchers of Tennessee Williams. It is qualitative in nature and inductive method is employed to carry it out. Pessimistic discourse in the plays of Williams is crafted in such a way that it has to be decoded and deconstructed to make optimistic sense of them. The study explores optimism in the chaotic world portrayed in these plays. It proves that Williams’ art focuses on ceaseless struggle to bring order to chaos. Focusing on binary pairs of opposites like conscious/unconscious, fertility/sterility, hope/hopelessness, soul/body, self/other sanity/insanity, regeneration/degeneration, saviors/killers, reality/illusion etc. this study tries to uncover certain contradictions and instabilities in the plays of Williams. By deconstructing the warring forces of signification in these plays it searches optimism in the bleak situation of human life. If the individual is at war with the society, he is also at war with himself. He becomes a hero when he refuses the role of victim and tries to change the course of events with an act of rebellion. In his desire to become his own liberator, he tries to transform his world. The external forces pounce upon him to crush him but they fail before his insatiable desire for existence. In his struggle for a better world Williams’ protagonists sometime fail but such a failure becomes a blessing in disguise as it makes them better human beings with great knowledge of things around them. This is what brims the dramatic world of Williams with hope and expectancy. This study highlights the importance of determination in making the best of life in the worst situation. The agony of entrapment in the plays of Williams and self realization through the act of defiance to it, in fact, help us recognize our own humanity and our desire for a better world. Williams unveils the complex and unruly human feelings without ignoring their strength. The study critically evaluates the plays of Williams and claims that he is not a pessimist playwright because the saviors in his plays are more powerful than the killers. It explores how Williams seeks hope in creativity and regeneration in degeneration.