مولاناابواللیث صدیقی
مِلّی،دینی اورعلمی حلقوں میں یہ خبرانتہائی رنج وغم کے ساتھ سنی گئی کہ سابق امیرجماعت اسلامی ہند حضرت مولاناابواللیث صدیقی ندوی اس ماہ دسمبر ۱۹۹۰ء میں طویل علالت کے بعد انتقال فرماگئے ۔ اِنَّالِلّٰہِ وَاِنَّا اِلَیْہِ راجعُون۔
مرحوم مولانا ابواللیث بڑے جیدعالم دین تھے۔عربی کے اسکالر تھے۔ مگراس کے باوجود سادگی وشرافت کے پیکر مجسّم تھے۔خاموش طبع تھے مگر بلاکے ذہین اوردانشوروں میں ان کاشمار ہوتاتھا۔۱۹۴۷ء کے پُر آشوب دور میں انہوں نے ہندوستان ہی میں دین اسلام اورمسلمانوں کی خدمت کرنے کابیڑہ اٹھانے کاعزم مصمم کیا۔اوران کی اعلیٰ کارکردگی ، کامیاب قیادت سے ملّت کو فیض بھی حاصل ہوا۔
مفکّر ملّت حضرت مفتی عتیق الرحمن عثمانی ؒسے ان کوبڑا گہرا لگاؤ تھا۔ان کی تعظیم وتکریم کرتے تھے اورملّی مسائل میں ان کے مشوروں سے فیضیاب بھی ہوتے رہے۔خود حضرت مفتی صاحبؒ بھی ان کی علمی قابلیت کے معترف تھے۔ مجلس مشاورت کے سلسلے میں ان کی خدمات سے انہوں نے بھرپور استفادہ بھی حاصل کیا۔ان میں متانت کُوٹ کُوٹ کربھری ہوئی تھی۔ان کے انتقال پرعظیم مذہبی رہنما وعالم دین حضرت مولانا ابوالحسن علی میاں ندوی صاحب مدظلہٗ نے صحیح کہا ہے کہ ان کے انتقال سے ملّت ہند ایک سچّے رہنما مخلص انسان سے محروم ہوگئی ہے۔اﷲ تعالیٰ کروٹ کروٹ جنّت نصیب کرے۔اٰمین! [دسمبر ۱۹۹۰ء]
The research reveals significant insights cited by Ibn Al ‘Irāqī in his book "Toḥfah Al Taḥṣīl" on the illusions of Al-‘Alā'ī in his book "Jāmi Al Taḥṣīl". It highlights the scientific value of those illusions that Ibn Al-‘Irāqī pointed out. Several of them are related to narrators of hadith and their issues of hearing from their sheikhs. Many of those illusions are related to the chain narrators (isnad), the main text of the report (matn), or their position in the books of sunnah. There are no previous studies on this subject. I put the sequential insights I revel under headlines through which one can realize the illusions that Al-Ala'i fell in. I conclude with the perceptions that both Ibn Al-‘Iraqī and Al-‘Alā’ī have shared. I don’t mention my opinion after each insight for Ibn Al-‘Iraqī; however, my silence is an approval to what he said. When I went against him or it was important to mention any comment or information, I openly said my opinion and explained the reasons for my opposition. Some of the research findings are: Al-Ala'i ignores mentioning the narrator's gap (irsal), although Al-Mizzī mentions it in his book "Tahdhīb", or the scholars mention it before both of them. He describes the narrator having a gap (irsāl) and attributes it to Al-Mizzī. He added notes like "he didn't encounter him" and formulated expressions that weren’t mentioned by neither Al Dhahabī nor Al-Mizzī. He references a Ḥadīth to a book that it is not included in.
The aim of the present investigation was to determine the effect of salt stress on the physiology of twelve genotypes of tomato (Solanum lycopersicum L.), to study the genetic diversity among these genotypes and to transfer salt tolerant gene in the selected genotype through Agrobacterium mediated gene transformation method. The seeds of different tomato genotypes were obtained from Ayub Agricultural Research Institute (AARI), Faisalabad and from Horticulture Research institute (HRI), NARC, Islamabad. First experiment comprises study of plant growth, ionic content, ABA content of shoot and root and proline content at four different NaCl salinity level. The genotypes Avinash, NTH242 and Gol performed better at higher salt concentration as indicated by less inhibition of plant height, shoot and root fresh weight and shoot and root dry weight. There was less translocation of Na + to shoot but greater accumulation of K + and Ca 2+ concomitant with higher production of osmoregulant (proline) and endogenous ABA accumulation in shoot was recorded. Second experiment was conducted to study the genetic diversity among the twelve tomato genotypes through randomly amplified polymorphic DNA analyses. Twelve tomato genotypes were grouped in four clusters at similarity coefficient value of 0.88 on the basis of randomly amplified polymorphic DNA analysis. Cluster-I consisted of a single tomato genotype “NTH-242”. Cluster-II of three tomato genotypes Lyp No.1, BARI, Roma and Cluster-III grouped Pakit and Riogrande together. Cluster-IV composed of maximum tomato genotypes i.e. 88572, Gol, Nagina, Checo, Pant Bahar and Avinash. Third experiment deals with Agrobacterium mediated transformation of DREB 1A gene in tomato using tissue culture. Prior to transformation three tomato genotypes were subjected to tissue culture studies to select the best genotype for callus induction and regeneration. Hypocotyls and leaf discs were used as explant source and inoculated on Murashige and Skoog media having different hormonal combinations. The callus induction and regeneration frequency varied among genotypes and it was concluded that hypocotyls of tomato genotype Avinash produced maximum callus induction and regeneration frequency on MS media xiiisupplemented with IAA (0.5 mg/l) and Kin (1.5 mg/l). Agrobacterium mediated gene transformation was carried out in tomato genotype Avinash to transfer DREB 1A gene tolerant to salt stress. During the study it was observed that two min infection time with Agrobacterium, two days for co-cultivation, Acetosyringone @ 50 μM in co-cultivated media and 50 mg/l hygromycin in selection media was optimum for high frequency transformation. Polymerase chain reaction (PCR) analysis was conducted to confirm trasfomants by amplification of 500 bp size DNA fragment for hygromycin resistance gene.