Comparative Effect Of Tetrapack Juices And Fresh Fruit Juices On Blood Glucose Level Among Healthy Individuals Comparative Effect of Juices on Blood Glucose Level

Uses of tetra pack juices enhances the risk of diabetes and obesity. People were unaware of impact of fresh fruit juices on health. Objective: To compare the effect of tetra pack juices and fresh fruit juices on blood glucoselevels among healthy individuals Methods: 10 participants were selected using an internationalstandard Glycemic Index (GI) test protocol. After getting their fasting blood sample theywereinstructed to consume all the juice served in a period of 5 min. Further blood samples were takenat different intervals of time that is 0, 30, 60, 90, 120 and 150 minutes after consumption. Participants wereremained sedentaryduring each session. Blood was obtained by finger-prick and tested by the glucometer. Results: The glycemic index of Apple juice Nestle has the glycemic index of 95.87, Orangejuice Nestle has the GI value 93.78, Apple juice fresh has the GI value of 92.17 and Orangejuice fresh has the glycemic index value of 99.07, respectively Conclusions: Detailed study of glycemic index of tetrapack juices (Nestle Apple and Orangejuice) and fresh fruit juices (Apple and Orange juice) showed similar impact on the blood glucose level of healthy individual. In the study, both types of juices were found to be equally hyperglycemic (GI 70+) causing a fast rise in blood-sugarlevels, hence should not be given to diabetic patients

Association of Rage Gene Polymorphism With Type 2 Diabetes Mellitus in Local Population

T2DM (Type 2 diabetes mellitus) is an endocrine disease having a significant genetic component.Polymorphisms of many genes may affect hereditary vulnerability of the disease that is characterized by insulin resistance and islet disorder. Though numerous factors associated with diabetes mellitus (DM) within local population have been assessed but gaps exist in determining genetic elements that contribute to this disease. As the genetic basis of T2DM can vary between ethnic groups, it is important to investigate the genetic link of T2DM in Pakistani populace. The existing project was aimed to assess the association of receptor for advanced glycation endproduct (RAGE) gene polymorphism (-429T>C and -374T/A) with type 2 diabetes mellitus within local populace. It employed 50 normal individuals and 100 patients suffering from type 2 diabetes mellitus (T2DM). Case control study involved demographic features and relative analysis associated with biochemical parameters (fasting blood sugar, HbA1C (glycated hemoglobin), liver function tests, lipid profile, renal function tests) within normal and diabetic individuals. Genotyping of the RAGE gene was studied by PCR- RFLP on genomic DNA. Among baseline characteristics, body mass index and blood pressure were notably (p<0.01) greater in case subjects in comparison with normal participants. Blood glucose, glycated hemoglobin and lipid profile showed substantial difference (p<0.01) between case and control subjects. Biochemical parameters had insignificant (p>0.01) relation with both genetic variants. Additionally RAGE gene polymorphism had non-significant association (p>0.05) with post diabetic complications. It is suggested that there might be no association of RAGE gene variants -429T>C and -374T/A with T2DM, diabetic retinopathy, nephropathy, cardiac and hypertensive manifestations in Pakistani population. To date this is the first national study as per our knowledge that throws light on the association of RAGE gene variants with T2DM and various biochemical parameters. Therefore, further exploration is needed to comprehend molecular and cellular systems of polymorphism which is still ambiguous. Additionally more genetic investigations might identify new candidate genes for treatment and prevention of the disease.