عناية أئمة النقد الحديثي بفقه الحديث

It is generally perceived in contemporary intellectual movements that canonical Traditionalists did not take had฀th text into consideration as their scholarly efforts were limited to the evaluation of had฀th chains. Aforementioned notion - in my opinion - originates from shallow study of methodology adopted by canonical had฀th critics، as a deeper look into their scholarly works reveals that sciences of had฀th includes the authentication and disparagement of traditions as well as comprehension and deduction from had฀th content. The sole objective of early Traditionalists from transmission، collection of had฀th، its evaluation، authentication and disparagement was to safeguard the true meaning of Sunnah and to transmit it in its pure form to the successors. In fact the peculiarity of their work is that they exert all efforts in order to deal with had฀th as a single undivided whole، where examination of content was not irrelevant to the evaluation of chain، their conscientious efforts recorded in major works of had฀th show how they evaluated content of had฀th to determine that it was not contradictory to Shari‘ah، or with another sound tradition، as there was a possibility that a certain reliable reporter made mistake or speculated in transmitting the meaning of had฀th. Therefore we witness them disparaging a certain transmitter for his negligence and errors whereas his had฀th is forsaken، moreover they would not consider him a Traditionalists or muhaddith฀n if excessive speculations were found in his report. This research paper aims at investigating the aforementioned hypothesis.

Exploration and Utlization of Promoters from Dicot Plats

The highly expressed promoters used in genetic engineering have been isolated from viruses. The commercially available promoters have been patented and the scientists have to address the Intellectual Property Rights issues related to patented promoters. The exploration of strong dicot promoters is needed for the expression of transgenes by our local researchers for the evaluation of their transgenes. The dicot promoters (β-TbBr promoter, β-TbSt promoter and susy-Sl promoter) were explored and isolated from potato, turnip and tomato respectively. The promoter sequences and their deletion mutants consist of various motifs for light response, hormonal response and stress response. Maximum light responsive motifs were detected in β-TbSt and susy-Sl promoters. The full length β-TbSt and susy-Sl promoters were expressed in tobacco and cotton tissues through transient assay and their expression was maximum in major tissues of these selected dicot plants. The strong β-Glucoronidase (GUS) expression was observed in roots, stem, leaves, flowers, trichomes and siliques of transgenic Arabidopsis thaliana under the β-TbSt and susy-Sl promoters. The full length β-TbBr promoter also expressed GUS gene in various tissues of tobacco and cotton through transient assay but the deletion mutants of this promoter were expressed only in anthers of transgenic lines of Arabidopsis. The deletion mutants of susy-Sl promoter showed strong constitutive GUS expression in roots, stems, leaves, flowers, trichomes and siliques of transgenic A.thaliana tissues.The smallest deletion mutant (679bp) of susy-Sl promoter showed optimal promoter activity in transgenic A. thaliana. The deletion mutants of β-TbSt promoter also expressed GUS gene in major tissues of A. thaliana but the expression was not good in roots due to removal of some of the root specific motifs and light inducible motifs. The β-TbBr promoter showed flower specific expression in A. thaliana. The β-TbSt promoter and susy-Sl promoter were found highly constitutive while the full length β-TbBr promoter can be utilized for the expression of salt inducible genes under stress conditions and the deletions of this promoter can be utilized for the floral specific expression of transgenes in plant genetic engineering.