1۔قتل عمد
کوئی شخص ، کسی کو ایسے ہتھیار سے مارے جس کی ضرب سے عام طور پر انسان مرجاتا ہے اور اس ضرب سے اس کو مارنے کا ارادہ بھی رکھتاہو، تو یہ قتل عمد کہلائے گا۔ یہ قتل کی سب سے مہلک قسم ہے اور اس پر سب سے زیادہ سزا رکھی گئی ہے تاکہ کوئی بھی انسانی جان کو قتل کرنے کی کو شش نہ کرے۔
From the very beginning Islam has given great importance to discipline. In every facet of life discipline has a role to play. Today when we delve deep in to our lives, we are amazed as to how it has always proved its worth. It has brought the tide of revolution in everyone's mind. Similarly, the element of discipline saves a society from disruption and instability. It infuses in life satisfaction and contentment. Contrarily, indiscipline and mismanagement give birth to dejection and dissatisfaction. Time management plays a vital role in the establishment of discipline in a society. Considering the vast importance of time management in the light of discipline, the following article throws a great deal of light on it in the Islamic perspective.
LMBR1 gene is consisted on a protein membrane and in human body it is re responsible for the limb development. As it is the promising gene for the limb development so recently many research is in process for exposure of potential application of this gene. The rapid increase of limb deformation in humans has led to more interest on the study of this gene from researcher and scholars. In this study we had taken the blood samples of 42 individuals including two families of limbs deformed were collected, age range of the patients was from 3 years to onwards, and had symptoms from moderate to severe from different areas of District Bahawalnagar. Physical sings were used as the selection criteria. 3.5-4ml venous blood was drawn and stored into 50ml tube containing 400?l EDTA, labeled with code number. The results obtained through different characterization e.g. (inorganic method, DNA estimation, sequencing of PCR product and electrophoresis) for LMBR1 gene exon 3 and 4. Thermo-cycler programs for the amplification of primers were not show any mutation in premier exons. Gel was visualized and in trans-illuminator and hence to ensure the amplified product. No further mutation has been observed in DNA strand. The DNA strands shows similar behavior of LMBR1 exon 4 and 3 for effected and normal individual. The mutation in this specified LMBR1 gene is not responsible for Limb deformity. Therefore LMBR1 gene is not accountable for any mutation caused limb deformity in an individual.