طالب حسین کوثریؔ کی نعت گوئی
نعت عربی زبان کا لفظ ہے جس کے معنی تعریف یا وصف بیان کرنا کے ہیں اگرچہ عربی زبان میں اس مقصد کیلئے مدح کا لفظ استعمال کیا جاتا ہے۔مگر ادبی اصطلاح میں لفظ نعت نبی کریمﷺ کی مدح و تعریف کیلئے استعمال کیا جاتا ہے۔فارسی لغات میں نعت سے مراد نبی کریمﷺ کے اوصاف اور ثناء بیان کرنا کے ہیں۔اردو زبان کی ’’نور اللغات‘‘ میں لکھا ہے۔کہ یہ لفظ معنی مطلق وصف ہے۔اور اس کا استعمال نبی پاکﷺکی تعریف و ثناء کے بیان کے لیے وقف ہے۔اس لیے نعت کا لفظ عربی،فارسی،اردو،پنجابی اور دیگر زبانوں میں ایسے ہی معنوں میں استعمال ہوتا ہے اور وہ سارے اشعار اور نظمیں نعت کے گھیرے میں آ جاتی ہیں جن میں آپﷺ کے محاسن بیان ہوئے ہوں۔اس طرح نعت کا گھیرا وسیع سے وسیع تر ہوتا جاتا ہے۔چونکہ نعت کا محور آپﷺ کی ذات پاک ہے۔اس لیے آپﷺ کی ذات سے صفات تک،افکار سے اعمال تک غرض ذات مقدس کا کوئی ایسا پہلو نہیں جو لغت کا موضوع نہ بنا ہو۔اخلاق ،سیرت،معجزات، غزوات، خطبات، عبادات، معاملات، عادات ،تعلیمات سب کچھ نعت کے گھیرے میں آ گئے ہیں۔
نعت کا وجود عربی ادب میں آپﷺ کی مبارک زندگی سے ہی نظر آتا ہے۔بہت سارے صحابہ کرام نے آپﷺکی پاک زندگی کے ایک ایک پل کو شعر وادب میں محفوظ کیا ہے۔یہی صحابہ کرام کا ورثہ تھا اور یہی ہمارا قیمتی اثاثہ ہے۔یوں تو اردو ادب میں لا تعداد شعراء کرام نے نبی پاکﷺ سے عقیدت و محبت کے اظہار کیلئے نعت گوئی کی،مگر بہت کم شعراء کرام ایسے ہیں جن کی ادب میں پہچان نعت گوئی بنی ہو۔دور حاضر کے ممتاز نعت گو شعراء کرام میں ایک نام جناب طالب حسین کوثریؔ کا بھی ہے۔جنہوں نے اپنی نعت...
, Officer Incharge/Senior Research Fellow, National Institute of Historical and Cultural Research, Centre of Excellence, Quaid-i-Azam University, Islamabad. Dr , Research Fellow, National Institute of Historical and Cultural Research, Centre of Excellence, Quaid-i-Azam University, Islamabad.
Plant secondary metabolites as biologically active compounds have a great future in controlling various degenerative diseases. The bioactive compounds certainly play a key role in effective medications. Bioactive compounds can be probed in the medicinal plants through in vitro and in vivo assays. In the past few decades, extensive work has been done to discover effective drugs from natural sources with low side effects. Due to medicinal significance in folk lore, eight medicinal plants (Albizia lebbeck, Melia azedarach, Hedera helix, Saxifraga flagellaris, Valeriana jatamansi, Fagonia cretica, Withania coagulans, and Moringa oleifera) were selected from the local flora to determine their anticancer potential and other biological activities. Initially, the plant extracts were obtained in 70% ethanol and were later fractionized into ethyl acetate, dichloromethane and n-hexane. The crude plant extracts were then used in various bioassays to determine their anti-bacterial, antifungal, phyto-toxic, anti-oxidant and anticancer potential. Also, the plant extracts were tested for proximate composition, heavy metals and phyto-chemicals. After, isolation and purification of compound, the compound of interest were utilized against cancer cell line (HT-29 Colon cancer cell lines). To estimate the antifungal activity against Aspergillus flavus, Alternaria alternate, Fusarium oxysporum, and Polysphondylium pallidum, an agar well diffusion method was adopted. The results showed that the crude methanolic extract of all plants were very effective against the selected fungal species. Melia azedarach, plants extract showed highest zone of inhibition 30.0±0.67 mm to 65.0±0.45 mm , followed by Saxifraga flagellaris, 29.0±0.67 mm to 65.0±0.54 mm, Moringa oleifera, 23.0±0.78 mm to 60.0±0.22 mm and Hedera helix, 0±0.55 mm to 57.0±0.88 mm. Antibacterial activity was also determined with the help of agar well diffusion method. The bacterial strains Streptococcus mutans, Methicillin-Resistant Staphylococcus aureus (MRSA), Staphylococcus aureus (Gram-positive), and Serratia marcescens (Gramnegative), were used. The antibacterial activity revealed that the crude methanolic extract of all plants were effective against the tested bacterial strains. Fagonia cretica plants extract showed highest zone of inhibition ranging from 07.0±0.66 mm to 15.0±0.33 mm (28 to 57.69%), followed by Valeriana jatamansi (25 to 57.14%). To determine the phytotoxic activity of the crude extracts (10, 20, and 40 mg), Lactuca sativa was used as a test plant. The crude extract of all plants at 20 and 40 mg have restricted the radical and plumule growth of L. sativa. The proximate analysis of the selected medicinal plants displayed substantial quantities of basic nutrients, like proteins, carbohydrate, fat, moisture and ash contents. The ethyl acetate extract of the tested medicinal plant species presented highest DPPH scavenging activity. Moreover, significant amounts of alkaloids, saponins, glycosides, flavonoids and phenols have been noticed in the crude extract of these plants. The MTT bioassay against available cancer cell line (Colon cancer cells--- HT-29) showed that Saxifraga flagellaris has restricted the cancer cell growth by 90%, followed by Fagonia cretica (31%), Moringa oleifera (23%), Valeriana jatamansi (21%), Withania coagulans (16%) and Hedera helix (10%). The potent crude extract of Saxifraga flagellaris was then subjected to the compound isolation by chromatographic techniques and characterized by 1H NMR, 13C NMR, DEPT 90 and DEPT135. The isolated compound was identified as adlumidine with molecular formula C20H17NO6 and molecular weight of 367.357 amu. This compound was reported in Saxifraga flagellaris for the first time. The purified compound, adlumidine reduced the cell viability of HCT 116 and PC3 cells. This compound proved to be toxic against HCT 116 and PC3 cell lines at ≥ 20 µg/ml concentrations. Adlumidine increased early apoptosis to 19.5%, while boosted late apoptosis to 10.4%. Furthermore, adlumidine induced caspase-3- dependent apoptosis in HCT 116 and PC3 cell lines. From the results of present study, it is concluded that Saxifraga flagellaris has potent bioactive compound(s) that can potentially control cancer and other contagious diseases.