قدرت اﷲ شہاب
جناب محمد طفیل کی وفات کے کچھ ہی روز بعد جناب قدرت اﷲ شہاب کی رحلت کی خبر ملی، وہ برطانوی حکومت کے زمانہ کے آئی۔سی۔ایس تھے، ۱۹۴۷ء کے بعد پاکستان کے بڑے بڑے عہدوں پر مامور رہے، وہ جب پاکستان کے گورنر جنرل جناب غلام محمد صاحب کے سکریٹری تھے، تو پہلی بار ۱۹۵۵ء میں ان سے دارالمصنفین کے دفتری کام کے سلسلہ میں ملا، ایک روز گورنر جنرل ہاؤس میں دوپہر کا کھانا ہوا تو وہ بھی شریک ہوئے لیکن خاموش بیٹھے رہے، ان سے کھانا شروع کرنے کے لیے کہا گیا تو بولے آج شعبان کی پندرہویں تاریخ ہے، وہ نفل روزے سے ہیں، ان کی اس مذہبیت کا اثر دستر خوان کے تمام شرکاء پر رہا۔
۱۹۵۵ء سے پاکستان کا سفر برابر کرتا رہا، ان سے برابر ملاقاتیں ہوتی رہیں، دارلمصنفین کی مطبوعات کا جب باضابطہ حق طباعت و اشاعت حکومت پاکستان کو دیا جارہا تھا تو انھوں نے اس کی دفتری کاروائی کرنے میں بڑی سہولتیں پہنچائیں جس کے لیے دارلمصنفین ان کا بڑا ممنون ہوا، ان میں سرکاری افسر کی رعونت بالکل نہ تھی، ہر موقع پر بڑے متین، سنجیدہ اور بااخلاق نظر آئے، بولتے بہت کم تھے مگر سنتے سب کی تھے، اور حتیٰ الامکان مدد کیا کرتے تھے، ان کو انگریزی اور اردو لکھنے میں بڑی مہارت تھی، اردو ادب کا بڑا عمدہ مذاق رکھتے تھے لیکن اس کا اظہار اپنی گفتگوؤں میں نہ ہونے دیتے، ادبی حلقوں میں اپنی اردو تحریروں کے لیے مقبول تھے، دعا ہے کہ اﷲ تبارک و تعالیٰ ان کی نیکیوں، لوگوں کے ساتھ کرم گستریوں اور روز مرہ زندگی میں ان کی خوبیوں کی بدولت ان کو اپنی آغوش عفو و کرم میں لے کروہی جگہ عطا فرمائیں جو نیک بندوں کو اس کی بارگاہ میں ملا کرتی ہے، آمین۔...
There are countless cases of cruelty and abuse of animals in the present era which are not mentioned in Islamic law, It is a pity that most of these atrocities are taking place in the so-called civilized western countries. However, it is heartening to see that the protest against the cruel exploitation by many western animal rights activists is well organized and it is hoped that this will eventually prevail and the animals will be given their due legitimate rights will remain together. What is worrying is that the developing countries, most of which are Islamic countries, have begun to follow their Western masters. They use the West's (Intensive Farming Methods) pesticides, which are harmful to humans and animals and do more harm to the environment than benefit crops, and lead to millions of animals being traded, exported or Similarly, the experiments that are done with reference to new products that are behind the maximum profit and follow them in the form of other similar luxuries. This article describes instructional teachings offered by Islam in such matters, which are very helpful and useful in raising awareness.
The effective removal and control of tuberculosis (TB) disease can be achieved with early and accurate diagnosis. It accounts for a majority of deaths and loss of health status thus damaging the economy. The present diagnostics for TB are not very effective as their sensitivity and specificity are low. Therefore the tests with more diagnostic value need to be developed. Thus a study was planned to develop an indigenous technology by exploiting biotechnology tools, and a new emerging technique i.e. multiplex microbead immunoassay (MMIA). Six potential recombinant antigenic genes: ag85a, ag85b, ag85c, cfp-10, esat-6 and hspx of Mycobacterium tuberculosis (M. tuberculosis) were selected for this purpose and respective genes were transformed into expression strain Bl21DE3pLysS for overexpression of proteins. Expression of each antigen was optimized for various conditions like concentration of isopropyl beta-D-1-thiogalactopyranoside (IPTG), time and temperature. Expression of protein was then confirmed by Western blotting. After confirmation, proteins were overexpressed in bulk cultures and purified by using immobilized metal affinity chromatography (IMAC) by using Histidine-tag (His-tag). The purified proteins were quantified and used to coat on microbeads at different concentrations and were used for analysis of collected blood samples. The blood samples of TB patients and healthy controls were collected from Federal TB Centre, Rawalpindi, from the students of Pir Mehr Ali Shah - Arid Agriculture University Rawalpindi (PMAS-AAUR), Rawalpindi, Punjab, Pakistan and healthy controls from USA. The collected human blood samples were divided as tuberculin skin test negative (TST -ve) healthy controls, from Pakistan and USA (group 1), TST positive (group 2), reactivated TB patients xx(group 3) and time points of active TB patients who were diagnosed and were under treatment (group 4). The coated microbeads were then used to analyze the presence of antibodies against M. tuberculosis in the collected blood samples. The results of group 1, Pakistan and USA group (TST negative) showed in general the absence of antibodies against any of the six antigens used in the MMIA. In the group 2 (TST positive), low median fluorescence intensity (MFI) values were detected against all antigens. Further in group 3 (reactivated TB patients) highest MFI values were observed against all antigens whereas in group 4 (active TB patient time points) MFI values were higher than group 2 but lower than group 3. This shows MMIA is very specific in detection of TB. Therefore, based on this it may be concluded that these antigens can be used to develop MMIA. However, use of more antigens and standardization is required.